{"id":1493,"date":"2017-01-30T13:42:54","date_gmt":"2017-01-30T04:42:54","guid":{"rendered":"https:\/\/gikenbio.com\/2025test\/?page_id=1493"},"modified":"2025-09-12T16:48:28","modified_gmt":"2025-09-12T07:48:28","slug":"metabarcoding","status":"publish","type":"page","link":"https:\/\/gikenbio.com\/2025test\/dnaanalysis\/ngs\/environment\/metabarcoding\/","title":{"rendered":"\u74b0\u5883DNA \u7db2\u7f85\u7684\u89e3\u6790\uff08\u30e1\u30bf\u30d0\u30fc\u30b3\u30fc\u30c7\u30a3\u30f3\u30b0\u89e3\u6790\uff09"},"content":{"rendered":"

\"\u7db2\u7f85\u7684\"\u74b0\u5883DNA \u7db2\u7f85\u7684\u89e3\u6790\uff08\u30e1\u30bf\u30d0\u30fc\u30b3\u30fc\u30c7\u30a3\u30f3\u30b0\u89e3\u6790\uff09<\/h2>\n

\u6b21\u4e16\u4ee3\u30b7\u30fc\u30b1\u30f3\u30b5\u30fc\u3092\u7528\u3044\u305f\u89e3\u6790\u3067\u3059\u3002\u74b0\u5883\u4e2d\u306eDNA\u3092\u7db2\u7f85\u7684\u306a\u30d7\u30e9\u30a4\u30de\u30fc\u30bb\u30c3\u30c8\u3067PCR\u5897\u5e45\u3057\u305f\u5f8c\u3001\u30b7\u30fc\u30b1\u30f3\u30b7\u30f3\u30b0\u3057\u307e\u3059\u3002\u5f97\u3089\u308c\u305f\u5869\u57fa\u914d\u5217\u3092\u30c7\u30fc\u30bf\u30d9\u30fc\u30b9\u3068\u7167\u3089\u3057\u5408\u308f\u305b\u3001\u751f\u7269\u76f8\u3092\u89e3\u6790\u3057\u307e\u3059\u3002<\/p>\n

1. \u74b0\u5883DNA\u3000\u7db2\u7f85\u7684\u89e3\u6790\uff08\u30e1\u30bf\u30d0\u30fc\u30b3\u30fc\u30c7\u30a3\u30f3\u30b0\u89e3\u6790\uff09\u6982\u8981<\/h3>\n
\n\n\n\n\n\n\n\n
\u4fa1\u683c<\/th>\n27,000\u5186\/\u30b5\u30f3\u30d7\u30eb<\/span><\/strong>(\u7a0e\u5225\uff09
\n\u5e95\u751f\u52d5\u7269\u306e\u89e3\u6790\u306f\u300132,000\u5186\/\u30b5\u30f3\u30d7\u30eb<\/strong>(\u7a0e\u5225\uff09
\n\uff1c\u5185\u8a33\uff1e
\n\u30fb\u308d\u904e\u30004,000\u5186\/\u30b5\u30f3\u30d7\u30eb
\n\u30fbDNA\u62bd\u51fa\u30003,000\u5186\/\u30b5\u30f3\u30d7\u30eb
\n\u30fb\u30e1\u30bf\u30d0\u30fc\u30b3\u30fc\u30c7\u30a3\u30f3\u30b0\u89e3\u6790\u300020,000\u5186\/\u30b5\u30f3\u30d7\u30eb\uff08\u5e95\u751f\u52d5\u7269\u306e\u89e3\u6790\u306f25,000\u5186\/\u30b5\u30f3\u30d7\u30eb\uff09
\n\u203b30\u30b5\u30f3\u30d7\u30eb\u4ee5\u4e0a\u306710\uff05\u5f15\u304d\u300160\u30b5\u30f3\u30d7\u30eb\u4ee5\u4e0a\u306720\uff05\u5f15\u304d<\/strong><\/td>\n<\/tr>\n
\u7d0d\u671f<\/th>\n20\u55b6\u696d\u65e5
\n\u203b25\u30b5\u30f3\u30d7\u30eb\u4ee5\u4e0a\u306f\uff0b2\u55b6\u696d\u65e5\u300157\u30b5\u30f3\u30d7\u30eb\u4ee5\u4e0a\u306f\uff0b3\u55b6\u696d\u65e5\u5fc5\u8981\u3067\u3059\u3002<\/td>\n<\/tr>\n
\u7d0d\u54c1\u7269<\/th>\n\u4ee5\u4e0b\u306e\u30c7\u30fc\u30bf\u3092\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u5f62\u5f0f\u3067\u7d0d\u54c1\u3044\u305f\u3057\u307e\u3059\u3002
\n\u30fb\u5831\u544a\u66f8
\n\u30fb\u30b7\u30fc\u30b1\u30f3\u30b9\u751f\u30c7\u30fc\u30bf\uff08fastq\u5f62\u5f0f\uff09
\n\u30fb\u30c7\u30fc\u30bf\u89e3\u6790\u7d50\u679c\uff08\u30a8\u30af\u30bb\u30eb\u30d5\u30a1\u30a4\u30eb\uff09
\n\u30fbDDBJ\u306e\u30c7\u30fc\u30bf\u30d9\u30fc\u30b9\u767b\u9332\u306b\u5fc5\u8981\u306a\u30c7\u30fc\u30bf<\/td>\n<\/tr>\n
\u89e3\u6790\u7cfb\u304c\u3042\u308b
\n\u751f\u7269\u7a2e<\/span><\/th>\n		\u52d5\u7269(COI)
\n\u9b5a\u985e(cMiFish)\u203b1
\n\u54fa\u4e73\u985e(MiMammal)\u203b2
\n\u7bc0\u8db3\u52d5\u7269(gInsect)\u203b3
\n\u9ce5\u985e(gBird)\u203b3\uff655
\n\u4e8c\u679a\u8c9d(gClam)\u203b3
\n\u5dfb\u8c9d(gSnail)\u203b3
\n\u6709\u5c3e\u76ee<\u30a4\u30e2\u30ea\u985e\u3068\u30b5\u30f3\u30b7\u30e7\u30a6\u30a6\u30aa\u985e>(gSalamander)\u203b3
\n\u7121\u5c3e\u76ee(gFrog)\u203b3
\n\u74b0\u5f62\u52d5\u7269(gWorm)\u203b3
\n\u7dda\u866b((Ecdysozoa)\u203b3
\n\u9678\u4e0a\u690d\u7269(gPlant)\u203b3
\n\u5149\u5408\u6210\u751f\u7269(psbA)\u203b3
\n\u8910\u85fb(gKasso)\u203b3
\n\u7d05\u85fb(gKoso)\u203b3
\n\u771f\u6838\u751f\u7269 (18S rRNA 1422f-1642r \u307e\u305f\u306f 18S rRNA ver2\uff1aV8f-1510r \u203b4)
\n\u5e95\u751f\u52d5\u7269(gBenthos)\u203b3\u30fb6
\n\u4e0a\u8a18\u4ee5\u5916\u306e\u751f\u7269\u7a2e\u306b\u3064\u3044\u3066\u306f\u3054\u76f8\u8ac7\u304f\u3060\u3055\u3044\u3002
\n(\u3000 )\u306f\u4f7f\u7528\u3059\u308b\u30d7\u30e9\u30a4\u30de\u30fc\u30bb\u30c3\u30c8\u540d\u307e\u305f\u306f\u89e3\u6790\u5bfe\u8c61\u9818\u57df\u306e\u540d\u79f0\u3067\u3059\u3002<\/span><\/td>\n<\/tr>\n
\u30aa\u30d7\u30b7\u30e7\u30f3<\/th>\n\u30d7\u30e9\u30a4\u30de\u30fc\u8ffd\u52a0\uff1a +2,000\u5186\/\u4f9d\u983c(\u7a0e\u5225\uff09<\/strong>
\ncMiFish\u306b\u30e4\u30c4\u30e1\u30a6\u30ca\u30ae\u985e\u7528\u306e\u30d7\u30e9\u30a4\u30de\u30fc\u3092\u8ffd\u52a0\u3059\u308b\u30aa\u30d7\u30b7\u30e7\u30f3\u3067\u3059\u3002\u203b7<\/td>\n<\/tr>\n
<\/th>\nMiFish\u5b9a\u91cf\u89e3\u6790\uff1a10,000\u5186\/\u30b5\u30f3\u30d7\u30eb\u3000\u7d0d\u671f\uff0b5\u55b6\u696d\u65e5
\n<\/strong>DNA\u30b3\u30d4\u30fc\u6570\u3092\u7b97\u51fa\u3059\u308b\u30aa\u30d7\u30b7\u30e7\u30f3\u3067\u3059\u3002
\n<\/strong><\/td>\n<\/tr>\n<\/tbody>\n<\/table><\/div>\n

\u203b1\u30002020\u5e745\u6708\u3088\u308a\u30d7\u30e9\u30a4\u30de\u30fc\u30bb\u30c3\u30c8\u306e\u540d\u79f0\u30fb\u6df7\u5408\u5185\u5bb9\u304c\u5909\u308f\u308a\u307e\u3059\u3002
\n\u65e7\uff09\u6de1\u6c34\u7528MiFish\u2192MiFish-U-F\/R\u3068MiFish-E-F\/R (Miya et al., 2015)\u30921:1\u3067\u6df7\u5408
\n\u6d77\u6c34\u7528MiFish\u2192MiFish-U-F\/R\u3068MiFish-E-F\/R-v2\u3001MiFish-U2-F\/R (unpublished primer)\u30922:1:1\u3067\u6df7\u5408
\n\u65b0\uff09cMiFish\u2192MiFish-U-F\/R\u3068MiFish-E-F\/R-v2\u3001MiFish-U2-F\/R \u3001MiFish-Ayu&Wakasagi(unpublished primer)\u30924:2:2:1\u3067\u6df7\u5408
\n\u203b2\u3000\u5f0a\u793e\u3067\u306e\u30c6\u30b9\u30c8\u89e3\u6790\u306e\u7d50\u679c\u3001\u810a\u690e\u52d5\u7269\u5168\u822c\u304c\u691c\u51fa\u3055\u308c\u3066\u3044\u307e\u3059\u3002
\n\u203b3\u3000\u81ea\u793e\u3067\u958b\u767a\u3057\u305f\u30d7\u30e9\u30a4\u30de\u30fc\u30bb\u30c3\u30c8\u3067\u306e\u89e3\u6790\u3067\u3059\u3002
\n\u203b4\u30002025\u5e745\u6708\u3088\u308a\u6a19\u6e96\u30d7\u30e9\u30a4\u30de\u30fc\u306b\u8ffd\u52a0\u3057\u307e\u3057\u305f\u3002\u6700\u8fd1\u306e\u8ad6\u6587\u3067\u591a\u304f\u4f7f\u308f\u308c\u3066\u3044\u308b\u30d7\u30e9\u30a4\u30de\u30fc\u3067\u3059\u3002
\n\u203b5\u3000\u9ce5\u985e\u306f\u74b0\u5883DNA\u3078\u306e\u79fb\u884c\u304c\u5c11\u306a\u304f\u3001\u691c\u51fa\u304c\u56f0\u96e3\u306a\u50be\u5411\u306b\u3042\u308a\u307e\u3059\u3002
\n\u203b6\u3000\u7bc0\u8db3\u52d5\u7269\u3001\u74b0\u5f62\u52d5\u7269\u3001\u8c9d(\u4e8c\u679a\u8c9d\u30fb\u5dfb\u8c9d)\u3001\u5341\u811a\u7532\u6bbb\u985e\u304c\u89e3\u6790\u5bfe\u8c61\u3067\u3059\u3002\u30d7\u30e9\u30a4\u30de\u30fc\u914d\u5217\u3084PCR\u6761\u4ef6\u306f\u958b\u793a\u3057\u3066\u3044\u307e\u305b\u3093\u306e\u3067\u3001\u3042\u3089\u304b\u3058\u3081\u3054\u4e86\u627f\u304f\u3060\u3055\u3044\u3002
\ngBenthos\u306e\u89e3\u6790\u3067\u306f\u3001\u8907\u6570\u306e\u30d7\u30e9\u30a4\u30de\u30fc\u3092\u30df\u30c3\u30af\u30b9\u3057\u30661stPCR\u3092\u884c\u3044\u307e\u3059\uff08\u30de\u30eb\u30c1\u30d7\u30ec\u30c3\u30af\u30b9PCR\uff09\u3002PCR\u306e\u539f\u7406\u4e0a\u3001\u5b58\u5728\u91cf\u306e\u591a\u3044\u751f\u7269\u306eDNA\u306f\u5897\u5e45\u3055\u308c\u3084\u3059\u304f\u3001\u5b58\u5728\u91cf\u306e\u5c11\u306a\u3044\u751f\u7269\u306eDNA\u306f\u3001\u5897\u5e45\u3055\u308c\u306b\u304f\u304f\u306a\u308b\u53ef\u80fd\u6027\u304c\u3042\u308a\u307e\u3059\u3002
\n\u4f8b\uff09\u30b5\u30f3\u30d7\u30eb\u4e2d\u306b\u8c9d\u985e\u306eDNA\u304c\u591a\u6570\u5b58\u5728\u3057\u3001\u7bc0\u8db3\u52d5\u7269\u306eDNA\u304c\u5c11\u306a\u3044\u5834\u5408\u306f\u3001PCR\u3092\u884c\u3063\u3066\u3082\u8c9d\u985e\u7531\u6765DNA\u3070\u304b\u308a\u5897\u5e45\u3055\u308c\u3001\u7bc0\u8db3\u52d5\u7269\u7531\u6765\u306eDNA\u304c\u307b\u3068\u3093\u3069\u5f97\u3089\u308c\u307e\u305b\u3093\u3002\u3053\u306e\u5834\u5408\u3001\u7bc0\u8db3\u52d5\u7269\u7528\u30d7\u30e9\u30a4\u30de\u30fc\uff08gInsect\uff09\u306e\u307f\u3067PCR\u3092\u884c\u3046\u3068\u7bc0\u8db3\u52d5\u7269\u7531\u6765DNA\u304c\u5897\u5e45\u3055\u308c\u305f\u30b1\u30fc\u30b9\u304c\u3054\u3056\u3044\u307e\u3059\u3002
\n\u203b7\u3000\u74b0\u5883DNA\u5206\u6790\u6280\u8853\u3092\u7528\u3044\u305f\u6de1\u6c34\u9b5a\u985e\u8abf\u67fb\u624b\u6cd5\u306e\u624b\u5f15\u304d\u7b2c\uff11\u7248\u306b\u8a18\u8f09\u3055\u308c\u3066\u3044\u308b\u30d7\u30e9\u30a4\u30de\u30fc\u30bb\u30c3\u30c8\u3067\u306e\u89e3\u6790\u3067\u3059\u3002<\/p>\n

\"\u74b0\u5883DNA\u30dd\u30fc\u30bf\u30d6\u30eb\u30c1\u30e5\u30fc\u30d6\u30dd\u30f3\u30d7\"<\/a><\/p>\n

2. \u74b0\u5883DNA\u3000\u30e1\u30bf\u30d0\u30fc\u30b3\u30fc\u30c7\u30a3\u30f3\u30b0\u89e3\u6790\u306e\u6d41\u308c<\/h3>\n
\n\n\n\n\n\n\n\n\n\n\n\n\n
\"\"
\n\uff08\u304a\u5ba2\u69d8\uff09
\n\u30b5\u30f3\u30d7\u30eb\u306e\u9001\u4ed8<\/strong><\/td>\n		\u6c34\u30b5\u30f3\u30d7\u30eb(1 L)\u3092\u51b7\u8535\u4fbf<\/strong><\/span>\u3067\u9001\u4ed8\u4e0b\u3055\u3044\u3002<\/p>\n

\u51b7\u8535\u4fdd\u7ba1\u3068\u767a\u9001\u304c\u96e3\u3057\u3044\u5834\u5408\u306f\u4e0b\u8a18\u3092\u53c2\u8003\u306b\u3057\u3066\u304f\u3060\u3055\u3044\u3002
\n\u74b0\u5883DNA\u89e3\u6790\u7528\u306e\u6c34\u30b5\u30f3\u30d7\u30eb\u306e\u4fdd\u7ba1\u3068\u767a\u9001\u65b9\u6cd5\u306b\u3064\u3044\u3066<\/a><\/p>\n<\/td>\n<\/tr>\n

\u2193<\/td>\n<\/td>\n<\/tr>\n
\u308d\u904e<\/strong><\/td>\n\u30d5\u30a3\u30eb\u30bf\u30fc\u3092\u7528\u3044\u3066\u3001\u308d\u904e\u3092\u884c\u3044\u307e\u3059\uff08\u5f0a\u793e\u6a19\u6e96\u30d5\u30a3\u30eb\u30bf\u30fc\u306f\u3001\u30b9\u30c6\u30ea\u3079\u30af\u30b9-GP 0.22\u03bcm\u3067\u3059\u3002\u6c34\u306e\u6fc1\u5ea6\u304c\u9ad8\u3044\u306a\u3069\u3001\u6a19\u6e96\u30d5\u30a3\u30eb\u30bf\u30fc\u3060\u3051\u3067\u306f\u308d\u904e\u304c\u96e3\u3057\u3044\u5834\u5408\u306f\u3001\u30d7\u30ec\u30d5\u30a3\u30eb\u30bf\u30fc\u3082\u7528\u3044\u3066\u308d\u904e\u3092\u884c\u3044\u307e\u3059\uff09\u3002<\/td>\n<\/tr>\n
\u2193<\/td>\n<\/td>\n<\/tr>\n
DNA\u62bd\u51fa\uff08\u6ce81\uff09<\/strong><\/td>\n\u30d5\u30a3\u30eb\u30bf\u30fc\u306b\u6355\u96c6\u3055\u308c\u305f\u7269\u8cea\u304b\u3089DNA\u3092\u62bd\u51fa\u3057\u307e\u3059\u3002\u62bd\u51faDNA\u304b\u3089PCR\u963b\u5bb3\u7269\u8cea\u3092\u9664\u53bb\u3057\u305f\u5f8c\u3001DNA\u6fc3\u5ea6\u3092\u6e2c\u5b9a\u3057\u307e\u3059\u3002<\/td>\n<\/tr>\n
\u2193<\/td>\n<\/td>\n<\/tr>\n
\u30e1\u30bf\u30d0\u30fc\u30b3\u30fc\u30c7\u30a3\u30f3\u30b0\u89e3\u6790<\/strong><\/td>\n\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u4f5c\u88fd<\/strong><\/td>\n12S rRNA\u3084COI\u9818\u57df\u306a\u3069\u3092\u5bfe\u8c61\u3068\u3057\u3066\u30012-step tailed PCR\u3092\u884c\u3044\u3001\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u3092\u4f5c\u88fd\u3057\u307e\u3059\u3002\u305d\u306e\u5f8c\u3001\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u306e\u6fc3\u5ea6\u3068\u54c1\u8cea\u3092\u78ba\u8a8d\u3057\u307e\u3059\u30021stPCR\u306f\uff18\u53cd\u5fa9\u5b9f\u65bd\u3057\u307e\u3059\u3002<\/td>\n<\/tr>\n
\u2193<\/td>\n<\/td>\n<\/tr>\n
\u30b7\u30fc\u30b1\u30f3\u30b7\u30f3\u30b0\u89e3\u6790<\/strong><\/td>\nIllumina NextSeq1000\u307e\u305f\u306fMiSeq\u3092\u4f7f\u7528\u3057\u3066\u30b7\u30fc\u30b1\u30f3\u30b7\u30f3\u30b0\u89e3\u6790\u3092\u884c\u3044\u307e\u3059\u30022x300bp\u306epaired-end\u30ea\u30fc\u30c9\u3092\u53d6\u5f97\u3057\u307e\u3059\u3002<\/td>\n<\/tr>\n
\u2193<\/td>\n<\/td>\n<\/tr>\n
\u30c7\u30fc\u30bf\u89e3\u6790<\/strong><\/td>\nDADA2\u6cd5\u3092\u7528\u3044\u3066\u3001\u5f97\u3089\u308c\u305f\u30ea\u30fc\u30c9\u3092amplicon sequence variant (ASV)\u306b\u307e\u3068\u3081\u307e\u3059\u3002ASV\u3068\u30ea\u30fc\u30c9\u6570\u3092\u307e\u3068\u3081\u305f\u8868\u3092\u3054\u63d0\u4f9b\u3057\u307e\u3059\u3002\u307e\u305f\u3001\u30c7\u30fc\u30bf\u30d9\u30fc\u30b9\u306e\u914d\u5217\u306897%\u4ee5\u4e0a\u306e\u76f8\u540c\u6027\u304c\u3042\u308bASV\u306f\u3001\u7a2e\u540d\u3092\u4f75\u8a18\u3057\u307e\u3059\u3002<\/td>\n<\/tr>\n<\/tbody>\n<\/table><\/div>\n

\u6ce81\uff09\u62bd\u51fa\u3055\u308c\u305fDNA\u306f\u3001\u30e1\u30bf\u30d0\u30fc\u30b3\u30fc\u30c7\u30a3\u30f3\u30b0\u89e3\u6790\u3060\u3051\u3067\u306a\u304f \u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u89e3\u6790<\/a>\u306b\u3082\u4f7f\u7528\u3059\u308b\u3053\u3068\u304c\u53ef\u80fd\u3067\u3059(\u8ffd\u52a0\u6599\u91d1: 8,000\u5186\/\u30b5\u30f3\u30d7\u30eb\u30fb\u751f\u7269\u7a2e)\u3002<\/p>\n

3. \u74b0\u5883DNA\u3000\u30e1\u30bf\u30d0\u30fc\u30b3\u30fc\u30c7\u30a3\u30f3\u30b0\u89e3\u6790\u306e\u5177\u4f53\u4f8b<\/h3>\n

\u5404OTU\u306b\u5bfe\u3057\u3066\u3001\u691c\u51fa\u3055\u308c\u305f\u30ea\u30fc\u30c9\u6570\u3068\u5bfe\u5fdc\u3059\u308b\u751f\u7269\u7a2e\u3092\u793a\u3057\u305f\u300c\u30a8\u30af\u30bb\u30eb\u30c7\u30fc\u30bf\u300d\u3092\u63d0\u4f9b\u81f4\u3057\u307e\u3059\u3002\u307e\u305f\u3001\u30c7\u30fc\u30bf\u30d9\u30fc\u30b9\u306e\u914d\u5217\u306897%\u4ee5\u4e0a\u306e\u76f8\u540c\u6027\u304c\u3042\u3063\u305fASV\u306e\u307f\u76f8\u540c\u6027\u30c8\u30c3\u30d710\u307e\u3067\u548c\u540d\u3092\u660e\u8a18\u81f4\u3057\u307e\u3059\u3002<\/p>\n

\u4f8b1\uff09\u76f8\u6a21\u6e7e\u306b\u304a\u3051\u308b\u9b5a\u985e\u76f8\u306e\u63a8\u5b9a<\/strong>\u3000>>\u8a73\u3057\u3044\u30c7\u30fc\u30bf\u3092\u3054\u89a7\u3044\u305f\u3060\u3051\u307e\u3059\u3002<\/a> >>\u76f8\u6a21\u5ddd\u7248\u3082\u3042\u308a\u307e\u3059<\/a><\/p>\n

\"sagamiwan\"<\/p>\n

\"\u76f8\u6a21\u6e7e\"
\n\u5404\u30b5\u30f3\u30d7\u30eb\u5730\u70b9\u304a\u3051\u308b\u5404\u751f\u7269\u7a2e\u7531\u6765\u306e\u30ea\u30fc\u30c9\u6570\u3092\u793a\u3057\u3066\u3044\u307e\u3059\u3002\u30d7\u30e9\u30a4\u30de\u30fc\u306f\u3001MiFish (Miya et al., 2015)\u3092\u4f7f\u7528\u3057\u307e\u3057\u305f\u3002<\/p>\n

\u4f8b2\uff09\u54fa\u4e73\u985e\u76f8\u306e\u63a8\u5b9a<\/strong> >>\u8a73\u3057\u3044\u30c7\u30fc\u30bf\u3092\u3054\u89a7\u3044\u305f\u3060\u3051\u307e\u3059\u3002<\/a><\/p>\n

\"\u54fa\u4e73\u985e\u89e3\u6790\u7d50\u679c\"
\n\u5404\u30b5\u30f3\u30d7\u30eb\u5730\u70b9\u304a\u3051\u308b\u5404\u751f\u7269\u7a2e\u7531\u6765\u306e\u30ea\u30fc\u30c9\u6570\u3092\u793a\u3057\u3066\u3044\u307e\u3059\u3002\u30d7\u30e9\u30a4\u30de\u30fc\u306f\u3001MiMammal (Ushio et al., 2016)\u3092\u4f7f\u7528\u3057\u307e\u3057\u305f\u3002<\/p>\n

\u4f8b3\uff09\u7bc0\u8db3\u52d5\u7269\u76f8\u306e\u63a8\u5b9a<\/strong>\u3000 >>\u8a73\u3057\u3044\u30c7\u30fc\u30bf\u3092\u3054\u89a7\u3044\u305f\u3060\u3051\u307e\u3059\u3002<\/a><\/p>\n

\"\u7bc0\u8db3\u52d5\u7269\u76f8\u89e3\u6790\u30c7\u30fc\u30bf\"
\n\u5404\u30b5\u30f3\u30d7\u30eb\u5730\u70b9\u304a\u3051\u308b\u5404\u751f\u7269\u7a2e\u7531\u6765\u306e\u30ea\u30fc\u30c9\u6570\u3092\u793a\u3057\u3066\u3044\u307e\u3059\u3002\u30d7\u30e9\u30a4\u30de\u30fc\u306f\u3001Insect\uff08\u5f0a\u793e\u306b\u3066\u958b\u767a\uff09\u3092\u4f7f\u7528\u3057\u307e\u3057\u305f\u3002<\/p>\n

\u4f8b4\uff09\u52d5\u7269\u76f8\u306e\u63a8\u5b9a\u3000<\/strong>>>\u8a73\u3057\u3044\u30c7\u30fc\u30bf\u3092\u3054\u89a7\u3044\u305f\u3060\u3051\u307e\u3059\u3002<\/a><\/p>\n

\"COI\"
\n\u5404\u30b5\u30f3\u30d7\u30eb\u5730\u70b9\u304a\u3051\u308b\u5404\u751f\u7269\u7a2e\u7531\u6765\u306e\u30ea\u30fc\u30c9\u6570\u3092\u793a\u3057\u3066\u3044\u307e\u3059\u3002COI\u5897\u5e45\u30d7\u30e9\u30a4\u30de\u30fc(Leray et al., 2013)\u3092\u4f7f\u7528\u3057\u307e\u3057\u305f\u3002<\/p>\n

\n

4. \u30d1\u30f3\u30d5\u30ec\u30c3\u30c8<\/h3>\n<\/div>\n

\"eDNA\u30d1\u30f3\u30d5\u30ec\u30c3\u30c8\"<\/a>\u74b0\u5883DNA\u89e3\u6790\u306e\u30d1\u30f3\u30d5\u30ec\u30c3\u30c8PDF<\/a><\/p>\n

5. \u304a\u554f\u5408\u305b\u30fb\u304a\u898b\u7a4d\u308a\u4f9d\u983c<\/h3>\n

\u25cf\u304a\u554f\u5408\u305b\u30fb\u304a\u898b\u7a4d\u308a\u4f9d\u983c<\/h4>\n

\u304a\u898b\u7a4d\u308a\u4f9d\u983c\u30d5\u30a9\u30fc\u30e0<\/a>\u3092\u3054\u7528\u610f\u3057\u3066\u3044\u307e\u3059\u3002\u307e\u305f\u3001\u304a\u554f\u5408\u305b\u30d5\u30a9\u30fc\u30e0<\/a>\u3001\u30e1\u30fc\u30eb\uff08dna@gikenbio.com\uff09\u3001\u307e\u305f\u306f \u304a\u96fb\u8a71\uff08042-780-8333\uff09\u3067\u304a\u6c17\u8efd\u306b\u3054\u76f8\u8ac7\u304f\u3060\u3055\u3044\u3002<\/p>\n

\u25cf\u30b5\u30f3\u30d7\u30eb\u9001\u4ed8\u5148<\/h4>\n

\u682a\u5f0f\u4f1a\u793e\u751f\u7269\u6280\u7814
\n\u3012252-0154\u3000\u795e\u5948\u5ddd\u770c\u76f8\u6a21\u539f\u5e02\u7dd1\u533a\u9577\u7af9657\u3000\/\u3000TEL 042-780-8333
\n\u203b\u5e73\u65e5\u7740\u3067\u304a\u9001\u308a\u304f\u3060\u3055\u3044\u3002\u767a\u9001\u306f\u5143\u6255\u3044\u3067\u304a\u9858\u3044\u3057\u3066\u3044\u307e\u3059\u3002<\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"\u74b0\u5883DNA \u7db2\u7f85\u7684\u89e3\u6790\uff08\u30e1\u30bf\u30d0\u30fc\u30b3\u30fc\u30c7\u30a3\u30f3\u30b0\u89e3\u6790\uff09 \u6b21\u4e16\u4ee3\u30b7\u30fc\u30b1\u30f3\u30b5\u30fc\u3092\u7528\u3044\u305f\u89e3\u6790\u3067\u3059\u3002\u74b0\u5883\u4e2d\u306eDNA\u3092\u7db2\u7f85\u7684\u306a\u30d7\u30e9\u30a4\u30de\u30fc\u30bb\u30c3\u30c8\u3067PCR\u5897\u5e45\u3057\u305f\u5f8c\u3001\u30b7\u30fc\u30b1\u30f3\u30b7\u30f3\u30b0\u3057\u307e\u3059\u3002\u5f97\u3089\u308c\u305f\u5869\u57fa\u914d\u5217\u3092\u30c7\u30fc\u30bf\u30d9\u30fc\u30b9\u3068\u7167\u3089\u3057\u5408\u308f\u305b\u3001\u751f\u7269 […]","protected":false},"author":1,"featured_media":0,"parent":47,"menu_order":1,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-1493","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/gikenbio.com\/2025test\/wp-json\/wp\/v2\/pages\/1493","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/gikenbio.com\/2025test\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/gikenbio.com\/2025test\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/gikenbio.com\/2025test\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/gikenbio.com\/2025test\/wp-json\/wp\/v2\/comments?post=1493"}],"version-history":[{"count":119,"href":"https:\/\/gikenbio.com\/2025test\/wp-json\/wp\/v2\/pages\/1493\/revisions"}],"predecessor-version":[{"id":7015,"href":"https:\/\/gikenbio.com\/2025test\/wp-json\/wp\/v2\/pages\/1493\/revisions\/7015"}],"up":[{"embeddable":true,"href":"https:\/\/gikenbio.com\/2025test\/wp-json\/wp\/v2\/pages\/47"}],"wp:attachment":[{"href":"https:\/\/gikenbio.com\/2025test\/wp-json\/wp\/v2\/media?parent=1493"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}