{"id":2124,"date":"2017-08-28T01:44:35","date_gmt":"2017-08-27T16:44:35","guid":{"rendered":"https:\/\/gikenbio.com\/2025test\/?page_id=2124"},"modified":"2024-05-03T18:16:27","modified_gmt":"2024-05-03T09:16:27","slug":"blocking","status":"publish","type":"page","link":"https:\/\/gikenbio.com\/2025test\/dnaanalysis\/ngs\/amplicon\/blocking\/","title":{"rendered":"\uff1c\u30a2\u30f3\u30d7\u30ea\u30b3\u30f3\u30b7\u30fc\u30b1\u30f3\u30b9\u89e3\u6790\u5fdc\u7528\u7de8\uff1e\u30d6\u30ed\u30c3\u30ad\u30f3\u30b0\u30fb\u9078\u629e\u7684\u5897\u5e45"},"content":{"rendered":"<h2>DNA\u5897\u5e45\u963b\u5bb3\/\u9078\u629e\u7684\u5897\u5e45\u6280\u8853\u3092\u7528\u3044\u305f\u751f\u7269\u76f8\u306e\u89e3\u6790<\/h2>\n<p>\u98df\u6027\u89e3\u6790\u3084\u690d\u7269\u75c5\u539f\u83cc\u306e\u83cc\u53e2\u89e3\u6790\u306b\uff01<\/p>\n<h3 class=\"style3b\" style=\"border-top-color: #000000;\">1.C3 spacer\u4fee\u98fe\u3092\u7528\u3044\u305f\u98df\u6027\u89e3\u6790<\/h3>\n<p>\u89e3\u6790\u8cbb\u7528\u306f\u5b89\u4fa1\u3067\u3059\u304c\u3001\u5bbf\u4e3bDNA\u3092\u5b8c\u5168\u306b\u6291\u5236\u3059\u308b\u3053\u3068\u304c\u96e3\u3057\u304f\u3001\u307e\u305f\u7a2e\u7279\u7570\u7684\u306a\u30d6\u30ed\u30c3\u30ad\u30f3\u30b0\u3082\u56f0\u96e3\u3067\u3059\u3002\u4e88\u5099\u7684\u306a\u5b9f\u9a13\u306b\u5411\u3044\u3066\u3044\u307e\u3059\u3002<\/p>\n<p>\uff1c\u8ffd\u52a0\u6599\u91d1\uff1e<br \/>\nC3 spacer\u4fee\u98fe \u30d6\u30ed\u30c3\u30ad\u30f3\u30b0\u30d7\u30e9\u30a4\u30de\u30fc\u8a2d\u8a08\u30fb\u5408\u6210\u8cbb\u7528<br \/>\n<strong><span style=\"font-size: 15pt;\">20,000\u5186<\/span>\/\u751f\u7269\u7a2e\uff08\u7a0e\u5225\uff09<br \/>\n<\/strong>\u203bC3 spacer\u4fee\u98fe\u30d7\u30e9\u30a4\u30de\u30fc\u306e\u8a2d\u8a08\u30fb\u5408\u6210\u306f5\u55b6\u696d\u65e5\u7a0b\u5ea6\u304b\u304b\u308a\u307e\u3059\u3002<strong><br \/>\n<\/strong><\/p>\n<p class=\"well2\"><strong>\u25cf\u89e3\u6790\u306e\u5177\u4f53\u4f8b<\/strong><br \/>\n<strong>\u30a2\u30b8\u306e\u990c\u751f\u7269\u306e\u63a8\u5b9a\uff08COI\u9818\u57df\uff09<\/strong><br \/>\n\u30b9\u30fc\u30d1\u30fc\u3067\u8cfc\u5165\u3057\u305f\u30a2\u30b8\u306e\u5185\u81d3\u3092\u7528\u3044\u3066\u3001\u98df\u6027\u89e3\u6790\u3092\u884c\u3044\u307e\u3057\u305f\u3002<br \/>\n\u4e0b\u306b\u3001\u691c\u51fa\u3055\u308c\u305f\u751f\u7269\u7531\u6765\u306e\u30ea\u30fc\u30c9\u6570\u3092\u68d2\u30b0\u30e9\u30d5\u3067\u793a\u3057\u307e\u3059\u3002<img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-2146\" src=\"https:\/\/gikenbio.com\/2025test\/wp-content\/uploads\/2017\/08\/eb50698bfde81d02629bbd02850abecc.jpg\" alt=\"c3spacer\u4fee\u98fe\u3092\u7528\u3044\u305f\u30d6\u30ed\u30c3\u30ad\u30f3\u30b0\" width=\"573\" height=\"184\" srcset=\"https:\/\/gikenbio.com\/2025test\/wp-content\/uploads\/2017\/08\/eb50698bfde81d02629bbd02850abecc.jpg 573w, https:\/\/gikenbio.com\/2025test\/wp-content\/uploads\/2017\/08\/eb50698bfde81d02629bbd02850abecc-300x96.jpg 300w\" sizes=\"auto, (max-width: 573px) 100vw, 573px\" \/><\/p>\n<h3 class=\"style3b\">2.\u4eba\u5de5\u6838\u9178 Locked Nucleic Acid(LNA)\u3092\u7528\u3044\u305f\u98df\u6027\u89e3\u6790<\/h3>\n<p>\u89e3\u6790\u8cbb\u7528\u306f\u9ad8\u4fa1\u3067\u3059\u304c\u3001\u7a2e\u7279\u7570\u7684\u306a\u30d6\u30ed\u30c3\u30ad\u30f3\u30b0\u304c\u53ef\u80fd\u3067\u3059\u3002<\/p>\n<p>\uff1c\u8ffd\u52a0\u6599\u91d1\uff1e<br \/>\nLNA\u30d6\u30ed\u30c3\u30ad\u30f3\u30b0\u30d7\u30e9\u30a4\u30de\u30fc\u8a2d\u8a08\u30fb\u5408\u6210\u8cbb\u7528<br \/>\n<strong><span style=\"font-size: 15pt;\">60,000\u5186<\/span>\/\u751f\u7269\u7a2e\uff08\u7a0e\u5225\uff09<br \/>\n<\/strong>\u203bLNA\u542b\u6709\u30d7\u30e9\u30a4\u30de\u30fc\u306e\u8a2d\u8a08\u30fb\u5408\u6210\u306f10\u55b6\u696d\u65e5\u7a0b\u5ea6\u304b\u304b\u308a\u307e\u3059\u3002<\/p>\n<h3 class=\"style3b\" style=\"border-top-color: #000000;\">3.\u30da\u30d7\u30c1\u30c9\u6838\u9178Peptide Nucleic Acid(PNA)\u3092\u7528\u3044\u305f\u83cc\u53e2\u89e3\u6790<\/h3>\n<p>\u89e3\u6790\u8cbb\u7528\u306f\u9ad8\u4fa1\u3067\u3059\u304c\u3001\u7279\u7570\u7684\u306a\u30d6\u30ed\u30c3\u30ad\u30f3\u30b0\u304c\u53ef\u80fd\u3067\u3059\u3002<br \/>\n\u5f0a\u793e\u3067\u306f\u690d\u7269\u7531\u6765\u306e\u8449\u7dd1\u4f53\u3068\u30df\u30c8\u30b3\u30f3\u30c9\u30ea\u30a2\u306e\u5897\u5e45\u3092\u963b\u5bb3\u3059\u308bPNA\u3092\u7528\u3044\u305f\u89e3\u6790\u306b\u5bfe\u5fdc\u3057\u3066\u3044\u307e\u3059\uff0816S rRNA V3\/V4\u9818\u57df\u307e\u305f\u306fV4\u9818\u57df\u306e\u89e3\u6790\u306e\u307f\u53ef\u80fd\u3067\u3059\uff09\u3002<\/p>\n<p>\uff1c\u8ffd\u52a0\u6599\u91d1\uff1e<br \/>\n\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u8abf\u6574\u8cbb\u7528<br \/>\n<strong><span style=\"font-size: 15pt;\">15,000\u5186<\/span>\/8\u30b5\u30f3\u30d7\u30eb\uff08\u7a0e\u5225\uff09<\/strong><\/p>\n<p class=\"well2\"><strong>\u25cf\u89e3\u6790\u306e\u5177\u4f53\u4f8b<\/strong><br \/>\n<strong>\u7a2e\u5b50\u306e\u83cc\u53e2\u89e3\u6790\uff0816S rRNA V4\u9818\u57df\uff09<\/strong><br \/>\n\u7267\u8349\u306e\u7a2e\u5b50\u3092\u7528\u3044\u3066\u3001\u83cc\u53e2\u89e3\u6790\u3092\u884c\u3044\u307e\u3057\u305f\u3002<br \/>\n\u203b\u672c\u89e3\u6790\u3067\u306f\u3001\u7a2e\u5b50\u306b\u30df\u30c8\u30b3\u30f3\u30c9\u30ea\u30a2\u7531\u6765\u306eDNA\u304c\u5c11\u306a\u304f\u3001Anti-mitochondria\u306e\u52b9\u679c\u306f\u691c\u8a3c\u3067\u304d\u3066\u3044\u307e\u305b\u3093\u3002<br \/>\n<img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-2148\" src=\"https:\/\/gikenbio.com\/2025test\/wp-content\/uploads\/2017\/08\/pna.jpg\" alt=\"PNA\u3092\u7528\u3044\u305f\u30d6\u30ed\u30c3\u30ad\u30f3\u30b0\" width=\"526\" height=\"313\" srcset=\"https:\/\/gikenbio.com\/2025test\/wp-content\/uploads\/2017\/08\/pna.jpg 526w, https:\/\/gikenbio.com\/2025test\/wp-content\/uploads\/2017\/08\/pna-300x179.jpg 300w\" sizes=\"auto, (max-width: 526px) 100vw, 526px\" \/><\/p>\n<h3 class=\"style3b\" style=\"border-top-color: #000000;\">4.LNA\u3092\u7528\u3044\u305f\u690d\u7269\u5185\u751f\u7d30\u83cc\u53e2\u30fb\u690d\u7269\u5185\u751f\u7cf8\u72b6\u83cc\u53e2\u306e\u89e3\u6790<\/h3>\n<p>\u9e7f\u5150\u5cf6\u5927\u5b66\u8fb2\u5b66\u90e8\u306e\u6c60\u6c38\u5148\u751f\u30fb\u5883\u5148\u751f\u304c\u958b\u767a\u3057\u305f\u3001\u7279\u8a31\u7b2c5846496\u304a\u3088\u3073\u7279\u98582016-051621\u306e\u907a\u4f1d\u5b50\u5897\u5e45\u65b9\u6cd5\u3092\u4f7f\u7528\u3057\u307e\u3059\u3002<br \/>\n\u690d\u7269\u5185\u751f\u7d30\u83cc\u9078\u629e\u7684\u5897\u5e45\u30d7\u30e9\u30a4\u30de\u30fc\u306f16S rRNA V3\/V4\u9818\u57df\u307e\u305f\u306fV4\u9818\u57df\u306e\u89e3\u6790\u306e\u307f\u3001\u690d\u7269\u5185\u7cf8\u72b6\u83cc\u9078\u629e\u7684\u5897\u5e45\u30d7\u30e9\u30a4\u30de\u30fc\u306fgITS-ITS4\u306e\u89e3\u6790\u306e\u307f\u53ef\u80fd\u3067\u3059\u3002<\/p>\n<p>\uff1c\u8ffd\u52a0\u6599\u91d1\uff1e<br \/>\n\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u8abf\u6574\u8cbb\u7528<br \/>\n<strong><span style=\"font-size: 15pt;\">20,000\u5186<\/span>\/8\u30b5\u30f3\u30d7\u30eb\uff08\u7a0e\u5225\uff09<\/strong><\/p>\n<p>\uff1c\u8ffd\u52a0\u7d0d\u671f\uff1e<br \/>\n<strong><span style=\"font-size: 15pt;\">5\u55b6\u696d\u65e5<\/span><\/strong><\/p>\n<p class=\"well2\"><strong>\u25cf\u89e3\u6790\u306e\u5177\u4f53\u4f8b<\/strong><br \/>\n<strong>\u690d\u7269\u30b5\u30f3\u30d7\u30eb\u306e\u83cc\u53e2\u89e3\u6790\uff08ITS\u9818\u57df\uff09<\/strong><br \/>\nITS1\u304b\u3089ITS2\u9818\u57df\u3092PCR\u6cd5\u3067\u5897\u5e45\u5f8c\u3001gITS7\u3068ITS4\u3092\u7528\u3044\u3066ITS2\u9818\u57df\u306e\u307f\u3092\u518d\u5ea6\u5897\u5e45\u3057\u3066\u83cc\u53e2\u89e3\u6790\u3092\u884c\u3044\u307e\u3057\u305f\u3002<br \/>\n<img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-2150\" src=\"https:\/\/gikenbio.com\/2025test\/wp-content\/uploads\/2017\/08\/LNA.jpg\" alt=\"LNA\u3092\u7528\u3044\u305f\u30d6\u30ed\u30c3\u30ad\u30f3\u30b0\" width=\"556\" height=\"351\" srcset=\"https:\/\/gikenbio.com\/2025test\/wp-content\/uploads\/2017\/08\/LNA.jpg 556w, https:\/\/gikenbio.com\/2025test\/wp-content\/uploads\/2017\/08\/LNA-300x189.jpg 300w\" sizes=\"auto, (max-width: 556px) 100vw, 556px\" \/><\/p>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"DNA\u5897\u5e45\u963b\u5bb3\/\u9078\u629e\u7684\u5897\u5e45\u6280\u8853\u3092\u7528\u3044\u305f\u751f\u7269\u76f8\u306e\u89e3\u6790 \u98df\u6027\u89e3\u6790\u3084\u690d\u7269\u75c5\u539f\u83cc\u306e\u83cc\u53e2\u89e3\u6790\u306b\uff01 1.C3 spacer\u4fee\u98fe\u3092\u7528\u3044\u305f\u98df\u6027\u89e3\u6790 \u89e3\u6790\u8cbb\u7528\u306f\u5b89\u4fa1\u3067\u3059\u304c\u3001\u5bbf\u4e3bDNA\u3092\u5b8c\u5168\u306b\u6291\u5236\u3059\u308b\u3053\u3068\u304c\u96e3\u3057\u304f\u3001\u307e\u305f\u7a2e\u7279\u7570\u7684\u306a\u30d6\u30ed\u30c3\u30ad\u30f3\u30b0 [&hellip;]","protected":false},"author":1,"featured_media":0,"parent":41,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-2124","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/gikenbio.com\/2025test\/wp-json\/wp\/v2\/pages\/2124","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/gikenbio.com\/2025test\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/gikenbio.com\/2025test\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/gikenbio.com\/2025test\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/gikenbio.com\/2025test\/wp-json\/wp\/v2\/comments?post=2124"}],"version-history":[{"count":24,"href":"https:\/\/gikenbio.com\/2025test\/wp-json\/wp\/v2\/pages\/2124\/revisions"}],"predecessor-version":[{"id":6287,"href":"https:\/\/gikenbio.com\/2025test\/wp-json\/wp\/v2\/pages\/2124\/revisions\/6287"}],"up":[{"embeddable":true,"href":"https:\/\/gikenbio.com\/2025test\/wp-json\/wp\/v2\/pages\/41"}],"wp:attachment":[{"href":"https:\/\/gikenbio.com\/2025test\/wp-json\/wp\/v2\/media?parent=2124"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}