{"id":3383,"date":"2019-10-21T20:38:32","date_gmt":"2019-10-21T11:38:32","guid":{"rendered":"https:\/\/gikenbio.com\/2025test\/?page_id=3383"},"modified":"2025-08-29T15:34:56","modified_gmt":"2025-08-29T06:34:56","slug":"rnaseq-dnb","status":"publish","type":"page","link":"https:\/\/gikenbio.com\/2025test\/dnaanalysis\/ngs\/rnaseq-dnb\/","title":{"rendered":"RNA-seq\u89e3\u6790\uff08\u907a\u4f1d\u5b50\u767a\u73fe\u89e3\u6790\uff09"},"content":{"rendered":"

\"RNAseq\"<\/p>\n

 <\/p>\n

\"RNAseq\u5b89\u304f\u306a\u308a\u307e\u3059\"<\/p>\n

1.RNA-seq\u89e3\u6790\uff08\u907a\u4f1d\u5b50\u767a\u73fe\u89e3\u6790\uff09\u306e\u30b5\u30fc\u30d3\u30b9\u6982\u8981<\/h3>\n
\n\n\n\n\n\n\n\n\n\n\n\n
\u4fa1\u683c\uff08\u7a0e\u5225\uff09<\/th>\n\u771f\u6838\u751f\u7269\uff08S. cerevisiae<\/em>\u3092\u9664\u304f\uff09<\/td>\n40,000\u5186\/\u30b5\u30f3\u30d7\u30eb<\/span><\/strong><\/td>\n<\/tr>\n
Saccharomyces cerevisiae\u3000<\/em><\/td>\n60,000\u5186\/\u30b5\u30f3\u30d7\u30eb<\/span><\/strong>
\n\u5185\u8a33\uff09RNA-seq\u89e3\u679040,000\u5186+rRNA\u9664\u53bb\uff08S. cerevisiae<\/em>\u7528\uff0920,000\u5186<\/td>\n<\/tr>\n
\u539f\u6838\u751f\u7269<\/td>\n50,000\u5186\/\u30b5\u30f3\u30d7\u30eb<\/span><\/strong>
\n\u5185\u8a33\uff09RNA-seq\u89e3\u679040,000\u5186+rRNA\u9664\u53bb\uff08\u539f\u6838\u751f\u7269\u7528\uff0910,000\u5186<\/td>\n<\/tr>\n
\u203bS. cerevisiae<\/em>\u3068\u539f\u6838\u751f\u7269\u306f\u30aa\u30ea\u30b4dT\u3067mRNA\u3092\u7cbe\u88fd\u304c\u3067\u304d\u306a\u3044\u305f\u3081\u3001\u4e0a\u8a18\u306e\u901a\u308arRNA\u9664\u53bb\u304c\u5fc5\u8981\u3068\u306a\u308a\u307e\u3059\u3002
\n\u203b8\u30b5\u30f3\u30d7\u30eb\u4ee5\u4e0a\u30675%\u5f15\u304d\u300124\u30b5\u30f3\u30d7\u30eb\u4ee5\u4e0a\u306710\uff05\u5f15\u304d<\/td>\n<\/tr>\n
\u30c7\u30fc\u30bf\u91cf<\/th>\n4,000\u4e07\u30ea\u30fc\u30c9\/6 Gb<\/td>\n<\/tr>\n
\u7d0d\u671f<\/th>\n25\u55b6\u696d\u65e5\u3000\u203b\u30c7\u30fc\u30bf\u89e3\u6790\u4e0d\u8981\uff08\u751f\u30c7\u30fc\u30bf\u7d0d\u54c1\uff09\u306e\u5834\u5408\u306f20\u55b6\u696d\u65e5<\/td>\n<\/tr>\n
\u4f5c\u696d\u5185\u5bb9<\/th>\n\u30fb\u9001\u4ed8\u30b5\u30f3\u30d7\u30eb\uff08\u30c8\u30fc\u30bf\u30ebRNA\uff09\u306e\u54c1\u8cea\u78ba\u8a8d\uff08\u6ce81\uff09
\n\u30fb\u3010\u771f\u6838\u751f\u7269\uff08S. cerevisiae\u3092\u9664\u304f<\/em>\uff09\u3011\u30aa\u30ea\u30b4dT\u3067mRNA\u3092\u7cbe\u88fd
\n\u3010Saccharomyces cerevisiae<\/em>\u30fb\u539f\u6838\u751f\u7269\u3011\u5404\u5bfe\u8c61\u751f\u7269\u7528\u306e\u30ad\u30c3\u30c8\u3092\u7528\u3044\u3066rRNA\u9664\u53bb
\n\u30fb\u30e9\u30f3\u30c0\u30e0\u30d7\u30e9\u30a4\u30de\u30fc\u3067cDNA\u5408\u6210\u5f8c\u3001\u30b7\u30fc\u30b1\u30f3\u30b9\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u4f5c\u88fd
\n\u30fb\u30b7\u30fc\u30b1\u30f3\u30b7\u30f3\u30b0\u89e3\u6790\uff08DNBSEQ 150 bp\u30da\u30a2\u30a8\u30f3\u30c9\u89e3\u6790\uff09
\n\u30fb\u30c7\u30fc\u30bf\u89e3\u6790\uff08\u6ce82\uff09
\nBowtie2\/STAR\uff08\u6ce83\uff09\u30bd\u30d5\u30c8\u30a6\u30a7\u30a2\u3092\u7528\u3044\u3066\u53c2\u7167\u914d\u5217\u306b\u30de\u30c3\u30d4\u30f3\u30b0\u3044\u305f\u3057\u307e\u3059\u3002\u305d\u306e\u5f8c\u3001featureCount\u3092\u7528\u3044\u3066\u30ab\u30a6\u30f3\u30c8\u3092\u884c\u3063\u305f\u5f8c\u3001TPM\u6b63\u898f\u5316\u3092\u884c\u3044\u307e\u3059\u3002
\n\u203bStringTie\u3092\u7528\u3044\u305f\u89e3\u6790\u3082\u5bfe\u5fdc\u53ef\u80fd\u3067\u3059\u3002
\n\u203b\u30d2\u30c8\u30fb\u30de\u30a6\u30b9\u30fb\u30e9\u30c3\u30c8\u30fb\u30a4\u30cc\u30fb\u30cb\u30ef\u30c8\u30ea\u30fb\u30a2\u30ca\u30a6\u30b5\u30ae\u306e\u5834\u5408\u306f\u3001\u30ea\u30fc\u30c9\u30ab\u30a6\u30f3\u30c8\u3068\u6b63\u898f\u5316\u306e\u4ee3\u308f\u308a\u306b\u3001 \u878d\u5408\u907a\u4f1d\u5b50\u306e\u691c\u51fa\u3092\u884c\u3046\u3053\u3068\u3082\u53ef\u80fd\u3067\u3059\u3002\u30ea\u30fc\u30c9\u30ab\u30a6\u30f3\u30c8\u3068\u6b63\u898f\u5316\u3082\u884c\u3046\u5834\u5408\u306f\u300110,000\u5186\uff08\u7a0e\u5225\uff09\u304b\u304b\u308a\u307e\u3059\u3002<\/td>\n<\/tr>\n
\u7d0d\u54c1\u7269<\/th>\n\u4ee5\u4e0b\u306e\u30c7\u30fc\u30bf\u3092DVD\u3084USB\u7b49\u306e\u8a18\u9332\u5a92\u4f53\u306b\u4fdd\u5b58\u3057\u3066\u7d0d\u54c1\u3057\u307e\u3059\u3002
\n\u30fb\u5831\u544a\u66f8
\n\u30fb\u30b7\u30fc\u30b1\u30f3\u30b9\u751f\u30c7\u30fc\u30bf\uff08fastq\u5f62\u5f0f\uff09
\n\u30fb\u30de\u30c3\u30d4\u30f3\u30b0\u3068\u30ab\u30a6\u30f3\u30c8\u7d50\u679c\uff08\u30a8\u30af\u30bb\u30eb\u30d5\u30a1\u30a4\u30eb\uff09
\n\u30fb\u53c2\u7167\u914d\u5217\uff08fasta\u5f62\u5f0f\uff09\u3068\u907a\u4f1d\u5b50\u30a2\u30ce\u30c6\u30fc\u30b7\u30e7\u30f3\uff08gff\u5f62\u5f0f\uff09
\n\u30fbDDBJ\u306e\u30c7\u30fc\u30bf\u30d9\u30fc\u30b9\u767b\u9332\u306b\u5fc5\u8981\u306a\u30c7\u30fc\u30bf<\/p>\n

\u203b\u30c7\u30fc\u30bf\u91cf\u304c\u591a\u3044\u5834\u5408\u306fHDD\u7d0d\u54c1\u3068\u306a\u308a\u3001HDD\u4ee3\u3068\u3057\u3066\u5225\u901420,000\u5186\uff08\u7a0e\u5225\uff09\u304c\u304b\u304b\u308a\u307e\u3059\u3002\u304a\u624b\u6301\u3061\u306eHDD\u3092\u9001\u4ed8\u3044\u305f\u3060\u304d\u3001\u305d\u3061\u3089\u306b\u4fdd\u5b58\u3057\u3066\u7d0d\u54c1\u3059\u308b\u3053\u3068\u3082\u53ef\u80fd\u3067\u3059\u3002
\n\uff1cHDD\u7d0d\u54c1\u3068\u306a\u308b\u76ee\u5b89\uff1e\u751f\u30c7\u30fc\u30bf\u7d0d\u54c1\u306e\u5834\u5408\u306f40\u30b5\u30f3\u30d7\u30eb\u3001\u30c7\u30fc\u30bf\u89e3\u6790\u3042\u308a\u306e\u5834\u5408\u306f20\u30b5\u30f3\u30d7\u30eb<\/p>\n<\/td>\n<\/tr>\n

\u3054\u63d0\u4f9b\u30b5\u30f3\u30d7\u30eb<\/th>\n\u30c8\u30fc\u30bf\u30ebRNA 1 \u03bcg\u4ee5\u4e0a\uff08\uff1e50ng\/ul\u3001\uff1e20ul\uff09
\n\u203b\u30c9\u30e9\u30a4\u30a2\u30a4\u30b9\u3092\u540c\u68b1\u3057\u3001\u51b7\u51cd\u4fbf\u3092\u4f7f\u7528\u3057\u3066\u9001\u4ed8\u304f\u3060\u3055\u3044\u3002
\n\u203bRNA\u6fc3\u5ea6\u304c\u57fa\u6e96\u5024\u672a\u6e80\u306e\u5834\u5408\u306f\u3001\u300c\u5fae\u91cfRNA\u5bfe\u5fdc\u300d\u306e\u30aa\u30d7\u30b7\u30e7\u30f3\u3092\u3054\u89a7\u304f\u3060\u3055\u3044\u3002<\/td>\n<\/tr>\n
\u3054\u4f9d\u983c\u306e\u6d41\u308c<\/th>\n\u307e\u305a\u306f\u304a\u898b\u7a4d\u308a\u3092\u3054\u4f9d\u983c\u304f\u3060\u3055\u3044\u3002\u304a\u898b\u7a4d\u308a\u4f9d\u983c\u30d5\u30a9\u30fc\u30e0<\/a>\u3092\u3054\u7528\u610f\u3057\u3066\u3044\u307e\u3059\u3002<\/td>\n<\/tr>\n<\/tbody>\n<\/table><\/div>\n

\uff08\u6ce81\uff09rRNA\u306e\u5206\u89e3\u304c\u9032\u3093\u3067\u304a\u308a\u3001\u30b7\u30fc\u30b1\u30f3\u30b7\u30f3\u30b0\u89e3\u6790\u3092\u884c\u3063\u3066\u3082\u30c7\u30fc\u30bf\u304c\u5f97\u3089\u308c\u306a\u3044\u3068\u5224\u65ad\u3057\u305f\u5834\u5408\u306f\u3001\u4e2d\u6b62\u3055\u305b\u3066\u3044\u305f\u3060\u304d\u307e\u3059\uff08\u3053\u306e\u5834\u5408\u306e\u3054\u8acb\u6c42\u306f\u884c\u3044\u307e\u305b\u3093\uff09\u3002
\n\uff08\u6ce82\uff09\u53c2\u7167\u914d\u5217\u304c\u306a\u3044\u751f\u7269\u7a2e\u306f\u3001De novo<\/em> transcriptome assembly \u89e3\u6790\uff08\u30aa\u30d7\u30b7\u30e7\u30f3\/\u4ee5\u4e0b\u53c2\u7167\uff09\u3092\u884c\u3063\u3066\u767a\u73fe\u907a\u4f1d\u5b50\u30ab\u30bf\u30ed\u30b0\u3092\u4f5c\u6210\u3057\u3001\u53c2\u7167\u914d\u5217\u306e\u4ee3\u308f\u308a\u3068\u3057\u3066\u30de\u30c3\u30d4\u30f3\u30b0\u306b\u7528\u3044\u307e\u3059\u3002De novo<\/em> transcriptome assembly \u89e3\u6790\u3092\u5b9f\u65bd\u3057\u306a\u3044\u5834\u5408\u306f\u3001\u751f\u30c7\u30fc\u30bf\u3067\u306e\u7d0d\u54c1\u3068\u306a\u308a\u307e\u3059\u3002
\n\uff08\u6ce83\uff092024\u5e745\u6708\u306bhisat2\u304b\u3089STAR\u306b\u5909\u66f4\u3057\u307e\u3057\u305f\u3002<\/p>\n

2.\u30aa\u30d7\u30b7\u30e7\u30f3<\/h3>\n

2-1.\u30aa\u30d7\u30b7\u30e7\u30f3\uff08RNA\u62bd\u51fa\u30fbrRNA\u9664\u53bb\u30fb\u5fae\u91cfRNA\u5bfe\u5fdc\uff09<\/h4>\n
\n\n\n\n\n\n\n\n
\u9805\u76ee<\/th>\n\u5099\u8003<\/th>\n\u4fa1\u683c\uff08\u7a0e\u5225\uff09<\/th>\n\u8ffd\u52a0
\n\u7d0d\u671f<\/th>\n<\/tr>\n
RNA\u62bd\u51fa\uff08\u571f\u58cc\u30fb\u6c5a\u6ce5\u4ee5\u5916\uff09<\/td>\nQIAGEN\u793e\u306eRNeasy\u7b49\u3092\u4f7f\u7528\u3057\u3066\u3001\u30c8\u30fc\u30bf\u30ebRNA\u3092\u62bd\u51fa\u3057\u307e\u3059\u3002
\n\uff1c\u3054\u63d0\u4f9b\u30b5\u30f3\u30d7\u30eb\u91cf\u306e\u76ee\u5b89\uff1e
\n\u7d30\u83cc\uff1a\u7d30\u80de\u6570 1\u00d710^9\u7a0b\u5ea6\u3001\u6e7f\u91cd\u91cf 20 mg\u7a0b\u5ea6
\n\u9175\u6bcd\uff1a\u7d30\u80de\u6570 5\u00d710^7\u7a0b\u5ea6\u3001\u6e7f\u91cd\u91cf 25 mg\u7a0b\u5ea6
\n\u690d\u7269\uff08\u8449\uff09\uff1a\u6e7f\u91cd\u91cf 50 mg\u7a0b\u5ea6\u3001\u76f4\u5f841.5cm
\n\u52d5\u7269\u7d30\u80de\uff1a\u7d30\u80de\u6570 1\u00d710^7\u7a0b\u5ea6\u3001\u6e7f\u91cd\u91cf 20 mg\u7a0b\u5ea6
\n\u52d5\u7269\u7d44\u7e54\uff1a\u6e7f\u91cd\u91cf 20 mg\u7a0b\u5ea6
\n\uff1c\u30b5\u30f3\u30d7\u30eb\u306e\u4fdd\u7ba1\u6e29\u5ea6\u3068\u9001\u4ed8\u65b9\u6cd5\uff1e
\n\u25c7 RNAlater\u306a\u3069\u306e\u6838\u9178\u4fdd\u5b58\u6db2\u306b\u6d78\u3059\u5834\u5408<\/strong>
\n\u63a1\u53d6\u5f8c\u3059\u3050\u306b\u30b5\u30f3\u30d7\u30eb\u306b\u6838\u9178\u4fdd\u5b58\u6db2\u3092\u6dfb\u52a0\u3057\u307e\u3059\u3002
\n\u4e00\u6669\u306f\u51b7\u8535\u4fdd\u7ba1\uff084\u2103\uff09\u3057\u3001\u7fcc\u65e5\u306b\u51b7\u51cd\uff08-20\u2103\uff09\u4fdd\u7ba1\u306b\u3057\u307e\u3059\u3002
\n\u9001\u4ed8\u6642\u306f\u3001\u51b7\u51cd\u4fbf\u3092\u5229\u7528\u3057\u3001\u30c9\u30e9\u30a4\u30a2\u30a4\u30b9\u306f\u540c\u68b1\u305b\u305a\u306b\u9001\u4ed8\u3057\u3066\u304f\u3060\u3055\u3044
\n\uff08RNAlater \u306f-20\u2103\u4ee5\u4e0b\u3067\u51cd\u7d50\u3057\u3066\u3057\u307e\u3046\u305f\u3081\uff09\u3002
\n\u25c7 \u6838\u9178\u4fdd\u5b58\u6db2\u3092\u6dfb\u52a0\u3057\u306a\u3044\u5834\u5408<\/strong>
\n\u63a1\u53d6\u5f8c\u3059\u3050\u306b\u30b5\u30f3\u30d7\u30eb\u3092-80\u2103\u4fdd\u7ba1\u3057\u307e\u3059\u3002
\n\u9001\u4ed8\u6642\u306f\u3001\u51b7\u51cd\u4fbf\u3092\u5229\u7528\u3057\u3001\u5fc5\u305a\u30c9\u30e9\u30a4\u30a2\u30a4\u30b9\u3092\u540c\u68b1\u3057\u3066\u304f\u3060\u3055\u3044\u3002<\/td>\n		20,000\u5186\/\u30b5\u30f3\u30d7\u30eb<\/td>\n5\u55b6\u696d\u65e5<\/td>\n<\/tr>\n
RNA\u62bd\u51fa\uff08\u571f\u58cc\u30fb\u6c5a\u6ce5\uff09<\/td>\nRNeasy Powersoil TotalRNA kit\u3092\u4f7f\u7528\u3057\u3066\u3001\u30c8\u30fc\u30bf\u30ebRNA\u3092\u62bd\u51fa\u3057\u305f\u306e\u3061\u3001DNase\u51e6\u7406\u3092\u884c\u3044\u307e\u3059\u3002
\n\uff1c\u30b5\u30f3\u30d7\u30eb\u91cf\u306e\u76ee\u5b89\uff1e\u571f\u58cc\uff1a\u6e7f\u91cd\u91cf 1~2 g
\n\uff1c\u30b5\u30f3\u30d7\u30eb\u306e\u4fdd\u7ba1\u6e29\u5ea6\u3068\u9001\u4ed8\u65b9\u6cd5\uff1e\u4e0a\u8a18\u306e\u300c\u25c7<\/strong>\u6838\u9178\u4fdd\u5b58\u6db2\u3092\u6dfb\u52a0\u3057\u306a\u3044\u5834\u5408\u300d\u3068\u540c\u3058\uff08\u571f\u58cc\u306b\u6838\u9178\u4fdd\u5b58\u6db2\u6dfb\u52a0\u306f\u63a8\u5968\u3057\u306a\u3044\u3068\u30de\u30cb\u30e5\u30a2\u30eb\u306b\u8a18\u8f09\u304c\u3042\u308a\u307e\u3059\uff09<\/td>\n		24,000\u5186\/\u30b5\u30f3\u30d7\u30eb<\/td>\n7\u55b6\u696d\u65e5<\/td>\n<\/tr>\n
rRNA\u9664\u53bb\uff08\u539f\u6838\u751f\u7269\u5bfe\u8c61\uff09<\/td>\nVazyme\u793e<\/span>\u306eRibo-off rRNA Deletion Kit\u7b49\u3092\u4f7f\u7528\u3057\u3066\u3001rRNA\u3092\u9664\u53bb\u3057\u307e\u3059\u3002<\/td>\n10,000\u5186\/\u30b5\u30f3\u30d7\u30eb<\/td>\n\u306a\u3057<\/td>\n<\/tr>\n
rRNA\u9664\u53bb\uff08S. cerevisiae<\/em>\u5bfe\u8c61\uff09<\/td>\nsiTOOLs Biotech\u793e<\/span>\u306eriboPOOL\u7b49\u3092\u4f7f\u7528\u3057\u3066\u3001rRNA\u3092\u9664\u53bb\u3057\u307e\u3059\u3002<\/td>\n20,000\u5186\/\u30b5\u30f3\u30d7\u30eb<\/td>\n\u306a\u3057<\/td>\n<\/tr>\n
\u5fae\u91cfRNA\u5bfe\u5fdc\uff08\u771f\u6838\u751f\u7269\u306e\u307f\u53ef\uff09<\/td>\n\u5fae\u91cfRNA\uff08>1 ng\/ul\u3001>20 ul\uff09\u306b\u5bfe\u3057\u3066\u3001\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u8abf\u88fd\u3092\u884c\u3044\u307e\u3059\u3002\u52a3\u5316\u3057\u305fRNA\u3067\u306f\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u8abf\u88fd\u304c\u3067\u304d\u307e\u305b\u3093\u3002
\n\u307e\u305f\u3001\u5fae\u91cfRNA\u5bfe\u5fdc\u306f\u901a\u5e38\u306e\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u8abf\u88fd\u65b9\u6cd5\u3068\u7570\u306a\u308b\u305f\u3081\u3001\u8907\u6570\u30b5\u30f3\u30d7\u30eb\u3092\u3054\u4f9d\u983c\u3044\u305f\u3060\u304f\u969b\u306f\u3001\u3069\u3061\u3089\u304b\u306e\u8abf\u88fd\u65b9\u6cd5\u3067\u7d71\u4e00\u3059\u308b\u3053\u3068\u3092\u5f37\u304f\u63a8\u5968\u3057\u307e\u3059\uff08\u7d71\u4e00\u3057\u306a\u3044\u3068\u3001\u30b5\u30f3\u30d7\u30eb\u9593\u306e\u767a\u73fe\u91cf\u306e\u6bd4\u8f03\u304c\u3067\u304d\u306a\u304f\u306a\u308a\u307e\u3059\uff09\u3002
\n<\/span><\/td>\n		20,000\u5186\/\u30b5\u30f3\u30d7\u30eb<\/td>\n5\u55b6\u696d\u65e5<\/td>\n<\/tr>\n<\/tbody>\n<\/table><\/div>\n

2-2.\u30aa\u30d7\u30b7\u30e7\u30f3\uff08\u30c7\u30fc\u30bf\u89e3\u6790\uff09<\/h4>\n

\u26052024.09 \u76ee\u7684\u5225\u306b\u30d1\u30c3\u30b1\u30fc\u30b8\u5316\u3057\u307e\u3057\u305f\u2605<\/span><\/strong><\/p>\n

\n\n\n\n\n\n\n
\u9805\u76ee<\/th>\n\u5099\u8003<\/th>\n\u4fa1\u683c\uff08\u7a0e\u5225\uff09<\/th>\n\u8ffd\u52a0
\n\u7d0d\u671f<\/th>\n<\/tr>\n
\u767a\u73fe\u30d7\u30ed\u30d5\u30a1\u30a4\u30eb\u306e\u985e\u4f3c\u6027<\/td>\n\u767a\u73fe\u6bd4\u8f03\u89e3\u6790\u524d\u306b\u5404\u30b5\u30f3\u30d7\u30eb\u306e\u907a\u4f1d\u5b50\u30d7\u30ed\u30d5\u30a1\u30a4\u30eb\u306e\u985e\u4f3c\u6027\u3092\u3044\u304f\u3064\u304b\u306e\u624b\u6cd5\u3067\u53ef\u8996\u5316\u3057\u3001\u53cd\u5fa9\u5185\u3084\u53cd\u5fa9\u9593\u3067\u907a\u4f1d\u5b50\u767a\u73fe\u306e\u5dee\u3092\u78ba\u8a8d\u3057\u307e\u3059\u3002
\n(\u4e3b\u6210\u5206\u5206\u6790\u3001\u76f8\u95a2\u5206\u6790\u3001\u968e\u5c64\u7684\u30af\u30e9\u30b9\u30bf\u30ea\u30f3\u30b0)<\/td>\n		30,000\u5186
\n\u203b3\u30b5\u30f3\u30d7\u30eb\u4ee5\u4e0a\u3067\u89e3\u6790\u53ef<\/td>\n		\u306a\u3057<\/td>\n<\/tr>\n
\u767a\u73fe\u6bd4\u8f03\u89e3\u6790<\/td>\n\u7d71\u8a08\u89e3\u6790\u306b\u3088\u3063\u3066\u767a\u73fe\u91cf\u304c\u6709\u610f\u306b\u7570\u306a\u308b\u907a\u4f1d\u5b50\u7fa4\u3092\u7279\u5b9a\u3057\u305f\u5f8c\u3001\u305d\u308c\u3092MA plot\u3084Volcano plot\u3001\u30d2\u30fc\u30c8\u30de\u30c3\u30d7\u3067\u63cf\u5199\u3057\u307e\u3059\u3002<\/td>\n1\u6bd4\u8f03\u76ee30,000\u5186
\n\u203b\u8ffd\u52a01\u6bd4\u8f03\u3054\u3068+10,000\u5186<\/td>\n		\u306a\u3057<\/td>\n<\/tr>\n
\u2517 \u30a8\u30f3\u30ea\u30c3\u30c1\u30e1\u30f3\u30c8\u89e3\u6790<\/td>\n\u767a\u73fe\u5909\u52d5\u3057\u305f\u907a\u4f1d\u5b50\u7fa4\u304c\u3001\u3069\u306e\u3088\u3046\u306a\u6a5f\u80fd\u3092\u3082\u3064\u306e\u304b\u4f53\u7cfb\u7684\u306b\u5206\u985e\u3057\u3001\u6709\u610f\u5dee\u691c\u5b9a\u3092\u884c\u3044\u307e\u3059\u3002\u307e\u305f\u3001KEGG\u306e\u4ee3\u8b1d\u30de\u30c3\u30d7\u4e0a\u306b\u53ef\u8996\u5316\u3059\u308b\u305f\u3081\u306e\u60c5\u5831\u3092\u7d0d\u54c1\u3057\u307e\u3059\u3002
\n(Gene Ontology(GO)\u89e3\u6790\u3001\u30d1\u30b9\u30a6\u30a7\u30a4\u89e3\u6790\u3001KEGG pathway classification chart)<\/td>\n		1\u6bd4\u8f03\u76ee50,000\u5186
\n\u203b\u8ffd\u52a01\u6bd4\u8f03\u3054\u3068+10,000\u5186<\/td>\n		\u306a\u3057<\/td>\n<\/tr>\n
De novo<\/em> transcriptome assembly<\/td>\n\u53c2\u7167\u914d\u5217\u304c\u306a\u3044\u975e\u30e2\u30c7\u30eb\u751f\u7269\u306e\u907a\u4f1d\u5b50\u30ab\u30bf\u30ed\u30b0\u914d\u5217\u3092\u3001Trinity\u3067\u69cb\u7bc9\u3057\u307e\u3059\u3002<\/td>\n100,000\u5186\/\u751f\u7269\u7a2e<\/td>\n\u306a\u3057<\/td>\n<\/tr>\n<\/tbody>\n<\/table><\/div>\n

3.\u5177\u4f53\u4f8b<\/h3>\n

\u6a19\u6e96\u89e3\u6790<\/h4>\n

\u907a\u4f1d\u5b50\u767a\u73fe\u884c\u5217\u30c7\u30fc\u30bf\u3092\u30a8\u30af\u30bb\u30eb\u30c7\u30fc\u30bf\u3067\u7d0d\u54c1\u3057\u307e\u3059\u3002>>\u8a73\u3057\u3044\u30c7\u30fc\u30bf\u3092\u3054\u89a7\u3044\u305f\u3060\u3051\u307e\u3059<\/a><\/p>\n

\u30b5\u30f3\u30d7\u30eb\u6bce\u306b\u3069\u306e\u907a\u4f1d\u5b50\u306b\u4f55\u30ea\u30fc\u30c9\u30de\u30c3\u30d4\u30f3\u30b0\u3055\u308c\u305f\u304b\u3092\u793a\u3057\u3066\u3044\u307e\u3059\u3002\u307e\u305f\u3001TPM\u6b63\u898f\u5316<\/a>\u5f8c\u306e\u6570\u5024\u3082\u793a\u3057\u3066\u3044\u307e\u3059\u3002<\/p>\n

\"RNAseq<\/p>\n

\u767a\u73fe\u30d7\u30ed\u30d5\u30a1\u30a4\u30eb\u306e\u985e\u4f3c\u6027\uff08\u30aa\u30d7\u30b7\u30e7\u30f3\uff09<\/h4>\n
\u4e3b\u6210\u5206\u5206\u6790<\/h5>\n

\u4e3b\u6210\u5206\u5206\u6790\u306e\u7d50\u679c\u3092png\u5f62\u5f0f\u3067\u7d0d\u54c1\u3057\u307e\u3059\u3002
\n<\/strong><\/p>\n

\"RNAseq\u4e3b\u6210\u5206\u5206\u6790\"<\/p>\n

\u76f8\u95a2\u56f3<\/h5>\n

\u76f8\u95a2\u56f3\u3092png\u5f62\u5f0f\u3067\u7d0d\u54c1\u3057\u307e\u3059\u3002<\/p>\n

\"\u76f8\u95a2\u56f3\"<\/p>\n

===\u56f3\u306e\u8aac\u660e===
\n\u53f3\u4e0a\u306e\u90e8\u5206\u306e\u5404\u6570\u5b57\u304cpearson\uff08\u30d4\u30a2\u30bd\u30f3\uff09\u306e\u76f8\u95a2\u95a2\u6570\u3067\u3059\u3002
\n\u6570\u5b57\u53f3\u4e0a\u306e *<\/span> \u306fp-value\u3067\u3059\u3002*<\/span> <0.05,\u00a0 **<\/span> <0.01, ***<\/span> <0.001<\/p>\n

\u968e\u5c64\u7684\u30af\u30e9\u30b9\u30bf\u30ea\u30f3\u30b0<\/h5>\n

\u30af\u30e9\u30b9\u30bf\u30ea\u30f3\u30b0\u306e\u7d50\u679c\u3092png\u5f62\u5f0f\u3067\u7d0d\u54c1\u3057\u307e\u3059\u3002
\n\"\u30af\u30e9\u30b9\u30bf\u30ea\u30f3\u30b0\"<\/p>\n

\u767a\u73fe\u6bd4\u8f03\u89e3\u6790\uff08\u30aa\u30d7\u30b7\u30e7\u30f3\uff09<\/h4>\n

1.\u907a\u4f1d\u5b50\u767a\u73fe\u6bd4\u8f03\u89e3\u6790\u306e\u7d50\u679c\u3092\u30a8\u30af\u30bb\u30eb\u30c7\u30fc\u30bf\u3067\u7d0d\u54c1\u3057\u307e\u3059\u3002<\/strong>>>\u8a73\u3057\u3044\u30c7\u30fc\u30bf\u3092\u3054\u89a7\u3044\u305f\u3060\u3051\u307e\u3059<\/a><\/p>\n

\"\"<\/p>\n

===\u30c7\u30fc\u30bf\u306e\u898b\u65b9===
\n-sample_name: \u6b63\u898f\u5316\u524d\u306e\u30ea\u30fc\u30c9\u30ab\u30a6\u30f3\u30c8
\n– a.value: log2(\u5e73\u5747\u767a\u73fe\u91cf)
\n– m.value: log2(\u767a\u73fe\u6bd4)
\n– p.value: p\u5024
\n– q.value: q\u5024 (false discovery rate(FDR))
\n– rank: p\u5024\u304c\u4f4e\u3044\u9806\u306b\u30e9\u30f3\u30af\u4ed8\u3051\u3055\u308c\u3066\u3044\u307e\u3059\u3002
\n– estmatedDEG: \u300c1\u300d\u306f\u767a\u73fe\u5909\u52d5\u907a\u4f1d\u5b50(q<0.05)\u3001\u300c0\u300d\u306f\u767a\u73fe\u5909\u52d5\u907a\u4f1d\u5b50\u3067\u306f\u306a\u3044\u3053\u3068(q>0.05)\u3092\u793a\u3057\u307e\u3059\u3002<\/p>\n

2.MA\u30d7\u30ed\u30c3\u30c8\u306e\u56f3\u3092png\u5f62\u5f0f\u3067\u7d0d\u54c1\u3057\u307e\u3059\u3002<\/strong>
\n\u6a2a\u8ef8\u304clog2(\u5e73\u5747\u767a\u73fe\u91cf(A))\u3001\u7e26\u8ef8\u304clog2(\u767a\u73fe\u6bd4(M))\u3092\u793a\u3057\u3066\u3044\u307e\u3059\u3002
\n\"MAplot\"<\/p>\n

Volcano\u30d7\u30ed\u30c3\u30c8<\/h5>\n

Volcano \u30d7\u30ed\u30c3\u30c8\u3092png\u5f62\u5f0f\u3067\u7d0d\u54c1\u3057\u307e\u3059\u3002
\nVolcano \u30d7\u30ed\u30c3\u30c8 \u306f\u3001MA\u30d7\u30ed\u30c3\u30c8\u306e\u89aa\u621a\u306e\u56f3\u3067\u3059\u3002volcano plot\u306f\u6a2a\u8ef8\u304clog2(\u767a\u73fe\u6bd4)\u3001\u7e26\u8ef8\u304cp\u5024\u3092\u793a\u3057\u3066\u3044\u307e\u3059\u3002<\/p>\n

\"\"<\/p>\n

\u30d2\u30fc\u30c8\u30de\u30c3\u30d7<\/h5>\n

\u30d2\u30fc\u30c8\u30de\u30c3\u30d7\u56f3\u3092png\u5f62\u5f0f\u3067\u7d0d\u54c1\u3057\u307e\u3059\u3002<\/p>\n

\"Heatmap\"<\/p>\n

\u30a8\u30f3\u30ea\u30c3\u30c1\u30e1\u30f3\u30c8\u89e3\u6790<\/h4>\n

\u30d1\u30b9\u30a6\u30a7\u30a4\u89e3\u6790<\/h5>\n

\u30d1\u30b9\u30a6\u30a7\u30a4\u89e3\u6790\u306e\u7d50\u679c\u3092\u30c6\u30ad\u30b9\u30c8\u30c7\u30fc\u30bf\u3067\u7d0d\u54c1\u3057\u307e\u3059\u3002\u30a8\u30af\u30bb\u30eb\u3067\u958b\u304f\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002>>\u8a73\u3057\u3044\u30c7\u30fc\u30bf\u3092\u3054\u89a7\u3044\u305f\u3060\u3051\u307e\u3059<\/a><\/p>\n

\"KEGG\"<\/p>\n

Gene Ontology\uff08GO\uff09\u89e3\u6790<\/h5>\n

Gene Ontology\uff08GO\uff09\u89e3\u6790\u306e\u7d50\u679c\u3092\u30a8\u30af\u30bb\u30eb\u30c7\u30fc\u30bf\u3067\u7d0d\u54c1\u3057\u307e\u3059\u3002<\/p>\n

\"GO\u89e3\u6790\u7d50\u679c\u4f8b\"<\/p>\n

4.\u3088\u304f\u3042\u308b\u8cea\u554f<\/h3>\n

FAQs\u30da\u30fc\u30b8<\/a>\u3092\u3054\u89a7\u304f\u3060\u3055\u3044\u3002<\/p>\n

5.\u3061\u3089\u3057PDF<\/h3>\n

\"RNAseq\"\u3000DNBSEQ\u3092\u7528\u3044\u305fRNA-seq\u89e3\u6790\u306e\u30d1\u30f3\u30d5\u30ec\u30c3\u30c8PDF<\/a><\/p>\n

6.\u304a\u554f\u5408\u305b\u30fb\u30b5\u30f3\u30d7\u30eb\u9001\u4ed8\u5148<\/h3>\n

\u304a\u898b\u7a4d\u308a\u30fb\u304a\u554f\u5408\u305b<\/h4>\n

\u304a\u898b\u7a4d\u308a\u30d5\u30a9\u30fc\u30e0<\/a>\u3001\u304a\u554f\u5408\u305b\u30d5\u30a9\u30fc\u30e0<\/a>\u3001\u30e1\u30fc\u30eb\uff08dna@gikenbio.com\uff09\u3001\u307e\u305f\u306f\u3000\u304a\u96fb\u8a71\uff08042-780-8333\uff09\u3067\u304a\u6c17\u8efd\u306b\u3054\u76f8\u8ac7\u304f\u3060\u3055\u3044\u3002<\/p>\n

\u30b5\u30f3\u30d7\u30eb\u9001\u4ed8\u5148<\/h4>\n

\u682a\u5f0f\u4f1a\u793e\u751f\u7269\u6280\u7814
\n\u3012252-0154\u3000\u795e\u5948\u5ddd\u770c\u76f8\u6a21\u539f\u5e02\u7dd1\u533a\u9577\u7af9657\u3000\/\u3000TEL\u3000042-780-8333
\n\u203b\u5e73\u65e5\u7740\u3067\u304a\u9001\u308a\u304f\u3060\u3055\u3044\u3002\u767a\u9001\u306f\u5143\u6255\u3044\u3067\u304a\u9858\u3044\u3057\u3066\u3044\u307e\u3059\u3002<\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"  1.RNA-seq\u89e3\u6790\uff08\u907a\u4f1d\u5b50\u767a\u73fe\u89e3\u6790\uff09\u306e\u30b5\u30fc\u30d3\u30b9\u6982\u8981 \u4fa1\u683c\uff08\u7a0e\u5225\uff09 \u771f\u6838\u751f\u7269\uff08S. cerevisiae\u3092\u9664\u304f\uff09 40,000\u5186\/\u30b5\u30f3\u30d7\u30eb Saccharomyces cerevisiae\u3000 60,000 […]","protected":false},"author":1,"featured_media":0,"parent":10,"menu_order":6,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-3383","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/gikenbio.com\/2025test\/wp-json\/wp\/v2\/pages\/3383","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/gikenbio.com\/2025test\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/gikenbio.com\/2025test\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/gikenbio.com\/2025test\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/gikenbio.com\/2025test\/wp-json\/wp\/v2\/comments?post=3383"}],"version-history":[{"count":103,"href":"https:\/\/gikenbio.com\/2025test\/wp-json\/wp\/v2\/pages\/3383\/revisions"}],"predecessor-version":[{"id":6967,"href":"https:\/\/gikenbio.com\/2025test\/wp-json\/wp\/v2\/pages\/3383\/revisions\/6967"}],"up":[{"embeddable":true,"href":"https:\/\/gikenbio.com\/2025test\/wp-json\/wp\/v2\/pages\/10"}],"wp:attachment":[{"href":"https:\/\/gikenbio.com\/2025test\/wp-json\/wp\/v2\/media?parent=3383"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}