{"id":41,"date":"2016-03-29T16:09:21","date_gmt":"2016-03-29T07:09:21","guid":{"rendered":"https:\/\/gikenbio.com\/2025test\/?page_id=41"},"modified":"2025-07-30T21:31:40","modified_gmt":"2025-07-30T12:31:40","slug":"amplicon","status":"publish","type":"page","link":"https:\/\/gikenbio.com\/2025test\/dnaanalysis\/ngs\/amplicon\/","title":{"rendered":"\u30a2\u30f3\u30d7\u30ea\u30b3\u30f3\u30b7\u30fc\u30b1\u30f3\u30b9\u89e3\u6790"},"content":{"rendered":"<h1><span style=\"color: red;\">\u3053\u308c\u307e\u3067\u30b7\u30fc\u30b1\u30f3\u30b5\u30fc\u306fMiSeq\u306e\u307f\u3067\u3057\u305f\u304c\u3001<br \/>\n<\/span><span style=\"color: red;\">2024\u5e748\u6708\u9803\u3088\u308aNextSeq1000\u304c\u7a3c\u50cd\u3057\u307e\u3057\u305f\u3002<br \/>\n<\/span><span style=\"color: red;\">\u3044\u305a\u308c\u3082\u30012\u00d7300 bp\u306e\u6761\u4ef6\u3067\u30b7\u30fc\u30b1\u30f3\u30b7\u30f3\u30b0\u3092\u884c\u3044\u307e\u3059\u3002<\/span><\/h1>\n<h1><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-6282\" src=\"https:\/\/gikenbio.com\/2025test\/wp-content\/uploads\/2024\/05\/962cf03585f3eeab73167feb0df5453f.jpg\" alt=\"\u30a2\u30f3\u30d7\u30ea\u30b3\u30f3\u30b7\u30fc\u30b1\u30f3\u30b9\u89e3\u6790\u4fa1\u683c\u8868\" width=\"798\" height=\"282\" srcset=\"https:\/\/gikenbio.com\/2025test\/wp-content\/uploads\/2024\/05\/962cf03585f3eeab73167feb0df5453f.jpg 798w, https:\/\/gikenbio.com\/2025test\/wp-content\/uploads\/2024\/05\/962cf03585f3eeab73167feb0df5453f-300x106.jpg 300w, https:\/\/gikenbio.com\/2025test\/wp-content\/uploads\/2024\/05\/962cf03585f3eeab73167feb0df5453f-768x271.jpg 768w\" sizes=\"auto, (max-width: 798px) 100vw, 798px\" \/><\/h1>\n<p class=\"well\"><strong>\u672c\u89e3\u6790\u30b5\u30fc\u30d3\u30b9\u306e\u7279\u5fb4<\/strong><br \/>\n\u30fb1\u30b5\u30f3\u30d7\u30eb\u3067\u3082\u3054\u6ce8\u6587\u53ef\u80fd\u3067\u3059\u3002<br \/>\n\u30fb\u30c7\u30fc\u30bf\u89e3\u6790\u307e\u3067\u30b5\u30fc\u30d3\u30b9\u306b\u542b\u307e\u308c\u305f\u30d1\u30c3\u30b1\u30fc\u30b8\u30b5\u30fc\u30d3\u30b9\u3067\u3059\u3002<br \/>\n\u30fb\u3059\u3079\u3066\u306e\u5de5\u7a0b\u3092\u56fd\u5185\uff08\u81ea\u793e\u5185\uff09\u3067\u884c\u3046\u304b\u3089\u3001\u77ed\u7d0d\u671f\u3067\u3059\u3002<br \/>\n\u30fb\u8178\u5185\u7d30\u83cc\u76f8\u3084\u571f\u58cc\u7d30\u83cc\u76f8\u306a\u3069\u306e\u5fae\u751f\u7269\u76f8\u3060\u3051\u3067\u306a\u304f\u3001\u3042\u3089\u3086\u308b\u751f\u7269\u76f8\u306b\u5bfe\u5fdc\u3002\u30d7\u30e9\u30a4\u30de\u30fc\u914d\u5217\u306e\u3054\u6307\u5b9a\u3082\u53ef\u80fd\u3067\u3059\u3002<\/p>\n\n<h3 class=\"style3b\">1.\u30a2\u30f3\u30d7\u30ea\u30b3\u30f3\u30b7\u30fc\u30b1\u30f3\u30b9\u89e3\u6790\u306e\u30b5\u30fc\u30d3\u30b9\u6982\u8981<\/h3>\n<div class=\"s_table\"><table>\n<tbody>\n<tr>\n<td><\/td>\n<th width=\"28%\">\u8a66\u6599\u3092\u9001\u308b<\/th>\n<th width=\"28%\">\u62bd\u51faDNA\u3092\u9001\u308b<\/th>\n<th width=\"28%\">1stPCR\u7523\u7269\u3092\u9001\u308b<\/th>\n<\/tr>\n<tr>\n<th>\u4fa1\u683c\uff08\u7a0e\u5225\uff09<\/th>\n<td>120,000\u5186\/8\u30b5\u30f3\u30d7\u30eb<\/td>\n<td>96,000\u5186\/8\u30b5\u30f3\u30d7\u30eb<\/td>\n<td>72,000\u5186\/8\u30b5\u30f3\u30d7\u30eb<\/td>\n<\/tr>\n<tr>\n<th>\u7d0d\u671f<\/th>\n<td>20\u55b6\u696d\u65e5\uff08\u6ce81\uff09<\/td>\n<td>15\u55b6\u696d\u65e5\uff08\u6ce81\uff09<\/td>\n<td>12\u55b6\u696d\u65e5\uff08\u6ce81\uff09<\/td>\n<\/tr>\n<tr>\n<th>\u4f5c\u696d\u5185\u5bb9<\/th>\n<td>DNA\u62bd\u51fa\uff5e\u30c7\u30fc\u30bf\u89e3\u6790<\/td>\n<td>DNA\u306e\u6fc3\u5ea6\u6e2c\u5b9a\uff5e\u30c7\u30fc\u30bf\u89e3\u6790<\/td>\n<td>1stPCR\u7523\u7269\u306e\u7cbe\u88fd\uff5e\u30c7\u30fc\u30bf\u89e3\u6790<\/td>\n<\/tr>\n<tr>\n<th rowspan=\"3\">\u3054\u63d0\u4f9b\u30b5\u30f3\u30d7\u30eb\u3068\u9001\u4ed8\u65b9\u6cd5<\/th>\n<td>\u8a66\u6599\uff08\u6ce82\uff09\uff1a\u5f0a\u793e\u63d0\u4f9b\u306e\u30c1\u30e5\u30fc\u30d6\u306e\u534a\u5206\u7a0b\u5ea6<\/td>\n<td>\u62bd\u51faDNA\uff1a\u6fc3\u5ea60.5 ng\/ul\u4ee5\u4e0a\u3001\u6db2\u91cf20 ul\u4ee5\u4e0a<br \/>\n\u203b\u6fc3\u5ea6\u306f\u3001\u86cd\u5149\u6cd5\u3092\u7528\u3044\u305f2\u672c\u9396DNA\u306e\u5b9a\u91cf\u7d50\u679c\u3092\u57fa\u6e96\u3068\u3057\u3066\u3044\u307e\u3059\u3002<br \/>\n260 nm\u306e\u5438\u5149\u5ea6\u30d9\u30fc\u30b9\u3067\u5b9a\u91cf\u3055\u308c\u3066\u3044\u308b\u5834\u5408\u3001\u4e0a\u8a18\u3088\u308a\u9ad8\u3081\u306e\u6fc3\u5ea6\uff085 ng\/ul\u4ee5\u4e0a\uff09\u304c\u671b\u307e\u3057\u3044\u3067\u3059\u3002<\/td>\n<td>1stPCR\u7523\u7269\uff08\u7cbe\u88fd\u524d\uff09\uff1a\u6db2\u91cf 15 ul\u4ee5\u4e0a<\/td>\n<\/tr>\n<tr>\n<td><img loading=\"lazy\" decoding=\"async\" class=\" wp-image-3674 alignright\" src=\"https:\/\/gikenbio.com\/2025test\/wp-content\/uploads\/2020\/05\/tube.png\" alt=\"\u7c89\u7815\u30c1\u30e5\u30fc\u30d6\" width=\"28\" height=\"48\" \/>\u5fc5\u305a\u5f0a\u793e\u63d0\u4f9b\u306e\u7c89\u7815\u75281.5 ml\u30c1\u30e5\u30fc\u30d6\u3092\u3054\u4f7f\u7528\u304f\u3060\u3055\u3044\u3002\u8a66\u6599\u306e\u7a2e\u985e\uff08\u571f\u58cc\u3001\u7cde\u4fbf\u306a\u3069\uff09\u3068\u6570\u3092\u304a\u77e5\u3089\u305b\u304f\u3060\u3055\u3044\u3002\u9001\u6599\u306f\u5f0a\u793e\u8ca0\u62c5\u3067\u304a\u9001\u308a\u3057\u307e\u3059\u3002<br \/>\n\uff1c\u91cf\u306e\u76ee\u5b89\uff1e<br \/>\n\u6e7f\u571f\u58cc\u3000200-500 mg\u7a0b\u5ea6<br \/>\n\u30de\u30a6\u30b9\u7cde\u4fbf\u3000\u7c73\u7c92\u59272-3\u7c92<\/td>\n<td>\u6fc3\u5ea6\u30920.5 ng\/ul\u306b\u8abf\u88fd\u3057\u3066\u3044\u305f\u3060\u304f\u5fc5\u8981\u306f\u3042\u308a\u307e\u305b\u3093\u3002\u307e\u305f\u3001\u57fa\u6e96\u6fc3\u5ea6\u672a\u6e80\u3067\u3082\u3001\u53d7\u3051\u5165\u308c\u3066\u3044\u307e\u3059\u3002<\/td>\n<td>\u30a2\u30c0\u30d7\u30bf\u30fc\u914d\u5217\u4ed8\u304d\u306e\u30d7\u30e9\u30a4\u30de\u30fc\u3092\u5f0a\u793e\u304b\u3089\u3054\u63d0\u4f9b\u3057\u307e\u3059\uff081,500\u5186 \u203b\u9001\u6599\u8fbc\uff09\u3002\u3054\u5e0c\u671b\u306e\u907a\u4f1d\u5b50\u9818\u57df\u3092\u304a\u77e5\u3089\u305b\u304f\u3060\u3055\u3044\u3002<br \/>\n=<br \/>\n1stPCR\u7523\u7269\uff08\u672a\u7cbe\u88fd\uff09\u306e\u6fc3\u5ea6\u6e2c\u5b9a\u306f\u4e0d\u8981\u3067\u3059\u3002\u3067\u304d\u308c\u3070\u96fb\u6c17\u6cf3\u52d5\u3092\u884c\u3044\u3001\u76ee\u7684\u306e\u5897\u5e45\u7523\u7269\u304c\u5f97\u3089\u308c\u3066\u3044\u308b\u3053\u3068\u3092\u3054\u78ba\u8a8d\u304f\u3060\u3055\u3044\u3002<\/td>\n<\/tr>\n<tr>\n<td>\u51b7\u51cd\u4fbf\u3067\u9001\u4ed8\u304f\u3060\u3055\u3044\u3002<\/td>\n<td colspan=\"2\">\u51b7\u8535\u4fbf or \u51b7\u51cd\u4fbf\u3067\u9001\u4ed8\u304f\u3060\u3055\u3044\uff08\u4fdd\u5b58\u6642\u3068\u540c\u3058\u6e29\u5ea6\u5e2f\u304c\u30d9\u30b9\u30c8\u3067\u3059\uff09\u3002<br \/>\n<img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-3677\" src=\"https:\/\/gikenbio.com\/2025test\/wp-content\/uploads\/2020\/05\/plate.png\" alt=\"tubeplate\" width=\"272\" height=\"74\" \/><br \/>\n\u30b5\u30f3\u30d7\u30eb\u6570\u304c\u591a\u3044\u3068\u304d\uff08\u76ee\u5b89\uff1a16\u30b5\u30f3\u30d7\u30eb\u4ee5\u4e0a\uff09\u306f8\u9023\u30c1\u30e5\u30fc\u30d6\u3084\u30d7\u30ec\u30fc\u30c8\u3067\u9001\u4ed8\u304f\u3060\u3055\u3044\u3002<\/td>\n<\/tr>\n<tr>\n<th>\u30b7\u30fc\u30b1\u30f3\u30b7\u30f3\u30b0\u6761\u4ef6<\/th>\n<td colspan=\"3\">illumina\u793eMiSeq\u307e\u305f\u306fNextSeq1000\u3092\u7528\u3044\u3066\u30012\u00d7300 bp\u306e\u6761\u4ef6\u3067\u30b7\u30fc\u30b1\u30f3\u30b7\u30f3\u30b0\u3092\u884c\u3044\u307e\u3059\uff08\u4ed6\u306e\u304a\u5ba2\u69d8\u3068\u76f8\u4e57\u308a\uff09\u3002<\/td>\n<\/tr>\n<tr>\n<th>\u30c7\u30fc\u30bf\u91cf<\/th>\n<td colspan=\"3\">1\u30b5\u30f3\u30d7\u30eb\u3042\u305f\u308a3\uff5e5\u4e07\u30ea\u30fc\u30c9\u30da\u30a2\u7a0b\u5ea6<\/td>\n<\/tr>\n<tr>\n<th>\u7d0d\u54c1\u7269<\/th>\n<td colspan=\"3\">\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u5f62\u5f0f\u3067\u306e\u7d0d\u54c1\u3068\u306a\u308a\u307e\u3059\u3002<br \/>\n\u5727\u7e2e\u5f8c\u306e\u30c7\u30fc\u30bf\u91cf\u304c2GB\u3092\u8d85\u3048\u308b\u5834\u5408\u306f\u8a18\u9332\u5a92\u4f53\u3067\u306e\u7d0d\u54c1\u306b\u5909\u66f4\u3055\u305b\u3066\u3044\u305f\u3060\u304d\u307e\u3059\uff08\u76ee\u5b89\u306f96\u30b5\u30f3\u30d7\u30eb\uff09<br \/>\n\u30fb\u5831\u544a\u66f8<br \/>\n\u30fb\u30b7\u30fc\u30b1\u30f3\u30b9\u751f\u30c7\u30fc\u30bf\uff08fastq\u5f62\u5f0f\uff09<br \/>\n\u30fb\u7cfb\u7d71\u89e3\u6790\u7d50\u679c<br \/>\n\u30fbDDBJ\u306e\u30c7\u30fc\u30bf\u30d9\u30fc\u30b9\u767b\u9332\u306b\u5fc5\u8981\u306a\u30c7\u30fc\u30bf<\/p>\n<p class=\"well\"><a class=\"q_button bt_red\" href=\"https:\/\/gikenbio.com\/2025test\/data\/funben_demo_Qiime2.zip\" target=\"_blank\" rel=\"noopener noreferrer\">\u7d0d\u54c1\u30c7\u30fc\u30bf\u306e\u30b5\u30f3\u30d7\u30eb<\/a><br \/>\n\u30d2\u30c8\u306e\u8178\u5185\u7d30\u83cc\u53e2\uff08\u89e3\u6790\u9818\u57df\u306f16SrRNA\u306eV3\/V4\u9818\u57df\uff09\u306e\u89e3\u6790\u7d50\u679c\u3067\u3059\u3002\u591a\u69d8\u6027\u89e3\u6790\u3068\u591a\u69d8\u6027\u89e3\u6790\u306e\u7d71\u8a08\u89e3\u6790\uff08\u3044\u305a\u308c\u3082\u30aa\u30d7\u30b7\u30e7\u30f3\uff09\u306e\u30c7\u30fc\u30bf\u3082\u542b\u307e\u308c\u3066\u3044\u307e\u3059\u3002<\/p>\n<\/td>\n<\/tr>\n<tr>\n<th>\u3054\u4f9d\u983c\u306e\u6d41\u308c<\/th>\n<td colspan=\"3\">\u307e\u305a\u306f\u304a\u898b\u7a4d\u308a\u3092\u3054\u4f9d\u983c\u304f\u3060\u3055\u3044\u3002<a href=\"https:\/\/gikenbio.com\/2025test\/dnaanalysis\/ngs\/amplicon\/quotation\/\" target=\"_blank\" rel=\"noopener\">\u304a\u898b\u7a4d\u308a\u4f9d\u983c\u30d5\u30a9\u30fc\u30e0<\/a>\u3092\u3054\u7528\u610f\u3057\u3066\u3044\u307e\u3059\u3002<\/td>\n<\/tr>\n<\/tbody>\n<\/table><\/div>\n<p>\u6ce81\uff09\u7d0d\u671f\u306f\u521d\u56de\u306ePCR\u3067\u826f\u597d\u306a\u5897\u5e45\u304c\u78ba\u8a8d\u3055\u308c\u305f\u5834\u5408\u3067\u3059\u3002\u518d\u8abf\u88fd\u304c\u5fc5\u8981\u306a\u5834\u5408\u306f\u8ffd\u52a0\u65e5\u6570\u3092\u3044\u305f\u3060\u304d\u307e\u3059\u30022\u30d7\u30ec\u30fc\u30c8\uff08192\u30b5\u30f3\u30d7\u30eb\uff09\u307e\u3067\u306f\u672c\u7d0d\u671f\u3067\u5bfe\u5fdc\u3057\u307e\u3059\u3002<br \/>\n\u6ce82\uff09\u5f0a\u793e\u304b\u3089\u3054\u63d0\u4f9b\u3055\u305b\u3066\u3044\u305f\u3060\u304f\u5c02\u7528\u30c1\u30e5\u30fc\u30d6\u306b\u5165\u308c\u3066\u767a\u9001\u3044\u305f\u3060\u304d\u307e\u3059\u3002\u30b5\u30f3\u30d7\u30eb\u306e\u5f62\u72b6\u7b49\u306b\u3088\u308a\u5f0a\u793e\u3067\u8ffd\u52a0\u306e\u4f5c\u696d\u304c\u5fc5\u8981\u3068\u5224\u65ad\u3057\u305f\u5834\u5408\u306f\u5225\u9014\u6599\u91d1\u3092\u3044\u305f\u3060\u304d\u307e\u3059\u3002\u307e\u305f\u3001\u30b5\u30f3\u30d7\u30eb\u306e\u5185\u5bb9\uff08\u4f8b\u3048\u3070\u611f\u67d3\u6027\u306e\u6709\u7121\uff09\u306b\u3088\u308a\u304a\u65ad\u308a\u3059\u308b\u5834\u5408\u304c\u3054\u3056\u3044\u307e\u3059\u3002<br \/>\nDNA \u62bd\u51fa\u306f\u3001\u5bfe\u8c61\u751f\u7269\u3084\u30b5\u30f3\u30d7\u30eb\u306e\u72b6\u6cc1\u306b\u3088\u3063\u3066\u62bd\u51fa\u65b9\u6cd5\u3092\u6700\u9069\u5316\u3059\u308b\u5fc5\u8981\u304c\u3054\u3056\u3044\u307e\u3059\u3002\u3088\u308a\u826f\u3044\u30c7\u30fc\u30bf\u3092\u5f97\u308b\u305f\u3081\u306e\u975e\u5e38\u306b\u91cd\u8981\u306a\u30b9\u30c6\u30c3\u30d7\u3067\u3042\u308b\u3068\u4f4d\u7f6e\u3065\u3051\u3066\u304a\u308a\u307e\u3059\u3002\u3053\u306e\u89b3\u70b9\u304b\u3089\u5f0a\u793e\u306b\u304a\u3051\u308bDNA\u62bd\u51fa\u30ce\u30a6\u30cf\u30a6\u306b\u4e4f\u3057\u3044\u30b5\u30f3\u30d7\u30eb\u306b\u3064\u3044\u3066\u306f\u304a\u65ad\u308a\u3055\u305b\u3066\u3044\u305f\u3060\u304f\u5834\u5408\u304c\u3054\u3056\u3044\u307e\u3059\u3002<\/p>\n<h3 class=\"style3b\">2.\u30a2\u30f3\u30d7\u30ea\u30b3\u30f3\u30b7\u30fc\u30b1\u30f3\u30b9\u89e3\u6790\u306e\u6d41\u308c<\/h3>\n<div class=\"s_table\"><table class=\"table_border_horizontal\">\n<tbody>\n<tr>\n<td class=\"flow\" width=\"25%\"><strong>DNA\u62bd\u51fa<\/strong><\/td>\n<td>\u5f0a\u793e\u306f\u3001\u7cde\u4fbf\u3084\u571f\u58cc\u3001\u6c34\u306a\u3069\u69d8\u3005\u306a\u30b5\u30f3\u30d7\u30eb\u304b\u3089\u306e\u62bd\u51fa\u5b9f\u7e3e\u304c\u3042\u308a\u3001\u305d\u308c\u305e\u308c\u306e\u30b5\u30f3\u30d7\u30eb\u306b\u6700\u9069\u306a\u62bd\u51fa\u65b9\u6cd5\u3067\u884c\u3044\u307e\u3059\u3002\u305d\u306e\u5f8c\u3001DNA\u6fc3\u5ea6\u3092\u6e2c\u5b9a\u3057\u307e\u3059\u3002<a href=\"https:\/\/gikenbio.com\/2025test\/look-up\/dna-extraction\/\" target=\"_blank\" rel=\"noopener noreferrer\">\uff1e\uff1e\u5f0a\u793e\u306eDNA\u62bd\u51fa\u65b9\u6cd5\u306b\u3064\u3044\u3066\u3001\u8a73\u3057\u304f\u306f\u3053\u3061\u3089<\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center;\">\u2193<\/td>\n<td><\/td>\n<\/tr>\n<tr>\n<td class=\"flow\"><strong>\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u4f5c\u88fd<\/strong><\/td>\n<td>PCR\u53cd\u5fdc\u3068\u7cbe\u88fd\u30922\u56de\u884c\u3044(2-step tailed PCR\uff09\u3001\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u3092\u4f5c\u88fd\u3057\u307e\u3059\u3002\u305d\u306e\u5f8c\u3001\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u306e\u6fc3\u5ea6\u3068\u54c1\u8cea\u3092\u78ba\u8a8d\u3057\u307e\u3059\u3002<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center;\">\u2193<\/td>\n<td><\/td>\n<\/tr>\n<tr>\n<td class=\"flow\"><strong>\u30b7\u30fc\u30b1\u30f3\u30b7\u30f3\u30b0\u89e3\u6790<\/strong><\/td>\n<td>MiSeq\u307e\u305f\u306fNextSeq 1000(Illumina)\u3092\u7528\u3044\u3066\u30012&#215;300 bp\u306e\u6761\u4ef6\u3067\u30b7\u30fc\u30b1\u30f3\u30b7\u30f3\u30b0\u3092\u884c\u3044\u307e\u3059\uff08\u4ed6\u306e\u304a\u5ba2\u69d8\u3068\u76f8\u4e57\u308a\uff09\u3002<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center;\">\u2193<\/td>\n<td><\/td>\n<\/tr>\n<tr>\n<td class=\"flow\"><strong>\u6a19\u6e96\u30c7\u30fc\u30bf\u89e3\u6790\uff08\u7cfb\u7d71\u89e3\u6790\uff09<\/strong><\/td>\n<td>\u83cc\u53e2\u89e3\u6790\u7528\u30d1\u30a4\u30d7\u30e9\u30a4\u30f3Qiime\u3092\u7528\u3044\u3066\u89e3\u6790\u3092\u884c\u3044\u3001\u300c\u83cc\u76f8\u7d44\u6210\u306e\u68d2\u30b0\u30e9\u30d5\u300d\u3084\u300c\u30d2\u30fc\u30c8\u30de\u30c3\u30d7\u300d\u306a\u3069\u306e\u5f62\u3067\u30c7\u30fc\u30bf\u306e\u53ef\u8996\u5316\u307e\u3067\u884c\u3044\u307e\u3059\u3002\u4e00\u90e8\u306e\u907a\u4f1d\u5b50\u9818\u57df(12S rRNA\u3084COI\u306a\u3069)\u306f\u3001\u300c\u30a8\u30af\u30bb\u30eb\u30c7\u30fc\u30bf\u300d\u306e\u307f\u3092\u63d0\u4f9b\u3044\u305f\u3057\u307e\u3059\u3002<br \/>\n\u203bQiime2.0\uff08DADA2\u30e2\u30c7\u30eb\uff09\u304c\u6a19\u6e96\u3067\u3059\u3002Qiime\u3067\u306e\u89e3\u6790\u3092\u3054\u5e0c\u671b\u306e\u65b9\u306f\u304a\u77e5\u3089\u305b\u304f\u3060\u3055\u3044\u3002\u8ffd\u52a0\u6599\u91d1\u306f\u767a\u751f\u3057\u307e\u305b\u3093\u3002\u203b<a href=\"https:\/\/gikenbio.com\/2025test\/2019\/05\/19\/qiime1%e3%81%a8qiime2%e3%81%ae%e9%81%95%e3%81%84%e3%81%ab%e3%81%a4%e3%81%84%e3%81%a6\/\" target=\"_blank\" rel=\"noopener noreferrer\">QIIME1\u3068QIIME2\u306e\u9055\u3044\u306b\u3064\u3044\u3066\u306f\u3053\u3061\u3089<\/a><br \/>\n&#8212;<strong><br \/>\n\u89e3\u6790\u306b\u7528\u3044\u308b\u30c7\u30fc\u30bf\u30d9\u30fc\u30b9<br \/>\n<\/strong><strong>\u25cf\u7d30\u83cc16S rRNA\uff1a<\/strong>Greengene\u30fbSILVA\u30fb<span class=\"wpcf7-form-control-wrap checkbox-database\"><span class=\"wpcf7-form-control wpcf7-checkbox\"><span class=\"wpcf7-list-item\"><span class=\"wpcf7-list-item-label\">Human Oral Microbiome Database<\/span><\/span><\/span><\/span>\u3088\u308a\u9078\u3079\u307e\u3059\u3002\u7279\u306b\u3054\u6307\u5b9a\u304c\u306a\u3044\u5834\u5408\u306f\u3001Greengene\u3092\u7528\u3044\u3066\u89e3\u6790\u3057\u307e\u3059\u3002<br \/>\nEzBioCloud 16S Database\u3092\u7528\u3044\u308b\u5834\u5408\u306f+10,000\u5186\/\u89e3\u6790\u304b\u304b\u308a\u307e\u3059\u3002<br \/>\n<strong>\u25cf\u771f\u83ccITS\uff1a<\/strong>UNITE<br \/>\n<strong>\u25cf18S rRNA\uff1a<\/strong>SILVA<br \/>\n<strong>\u25cfcMiFish\uff1a<\/strong>\u9b5a\u985e\u30df\u30c8\u30b3\u30f3\u30c9\u30ea\u30a2\u30b2\u30ce\u30e0\u30c7\u30fc\u30bf\u30d9\u30fc\u30b9MitoFish\u3068MiFish\u7528\u53c2\u7167\u914d\u5217<br \/>\n<strong>\u25cf\u305d\u306e\u4ed6\uff1a<\/strong>NCBI<\/td>\n<\/tr>\n<tr>\n<td class=\"flow\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-3699 alignright\" src=\"https:\/\/gikenbio.com\/2025test\/wp-content\/uploads\/2020\/05\/331f5f8bfcff24c5799e654c29dea477.png\" alt=\"QIIME2\u3068Ezbio\" width=\"67\" height=\"112\" srcset=\"https:\/\/gikenbio.com\/2025test\/wp-content\/uploads\/2020\/05\/331f5f8bfcff24c5799e654c29dea477.png 572w, https:\/\/gikenbio.com\/2025test\/wp-content\/uploads\/2020\/05\/331f5f8bfcff24c5799e654c29dea477-178x300.png 178w\" sizes=\"auto, (max-width: 67px) 100vw, 67px\" \/><\/td>\n<td><strong>\u30b2\u30ce\u30e0\u7de8\u96c6\u7d50\u679c\u306e\u78ba\u8a8d\u3084\u591a\u578b\u3092\u77e5\u308a\u305f\u3044\u65b9\u306b\u306f\u3001\u300cCRISPR-DAV\u3092\u7528\u3044\u305f\u89e3\u6790\u300d\u3084\u300c<a href=\"https:\/\/gikenbio.com\/2025test\/look-up\/custom-allele\/\" target=\"_blank\" rel=\"noopener\">\u30ab\u30b9\u30bf\u30e0\u591a\u578b\u89e3\u6790<\/a>\u300d<\/strong><strong>\u304c\u30aa\u30b9\u30b9\u30e1\u3067\u3059\u3002<br \/>\n<\/strong>\u3044\u305a\u308c\u3082\u8ffd\u52a0\u6599\u91d1\u306f\u304b\u304b\u308a\u307e\u305b\u3093\u3002\u304a\u898b\u7a4d\u308a\u6642\u306b\u3069\u3061\u3089\u304b\u3054\u5e0c\u671b\u3092\u304a\u77e5\u3089\u305b\u304f\u3060\u3055\u3044\u3002<\/td>\n<\/tr>\n<tr>\n<td colspan=\"2\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-3691 alignnone\" src=\"https:\/\/gikenbio.com\/2025test\/wp-content\/uploads\/2020\/05\/ba2405d2e495e41dc9d7926573be260d.jpg\" alt=\"Qiime\u7d0d\u54c1\u30c7\u30fc\u30bf\" width=\"660\" height=\"117\" srcset=\"https:\/\/gikenbio.com\/2025test\/wp-content\/uploads\/2020\/05\/ba2405d2e495e41dc9d7926573be260d.jpg 688w, https:\/\/gikenbio.com\/2025test\/wp-content\/uploads\/2020\/05\/ba2405d2e495e41dc9d7926573be260d-300x53.jpg 300w, https:\/\/gikenbio.com\/2025test\/wp-content\/uploads\/2020\/05\/ba2405d2e495e41dc9d7926573be260d-680x122.jpg 680w\" sizes=\"auto, (max-width: 660px) 100vw, 660px\" \/><br \/>\n\u2605\u30d6\u30e9\u30a6\u30b6\u4e0a\u3067\u30c7\u30e2\u30c7\u30fc\u30bf\u3092\u3054\u89a7\u3044\u305f\u3060\u3051\u307e\u3059\uff08<a href=\"https:\/\/gikenbio.com\/2025test\/sampledata\/qiime2\/taxa_summary_plots\/bar_chart.html\" target=\"_blank\" rel=\"noopener noreferrer\">\u83cc\u76f8\u7d44\u6210\u306e\u68d2\u30b0\u30e9\u30d5<\/a>\u30fb<a href=\"https:\/\/gikenbio.com\/2025test\/sampledata\/qiime2\/OTU_Heatmap\/otu_table.html\" target=\"_blank\" rel=\"noopener noreferrer\">OTU\u3000heatmap<\/a>\u30fb\u30b5\u30f3\u30d0\u30fc\u30b9\u30c8\u30c1\u30e3\u30fc\u30c8\uff09<strong><br \/>\n<\/strong><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-5430\" src=\"https:\/\/gikenbio.com\/2025test\/wp-content\/uploads\/2022\/05\/nohin.jpg\" alt=\"12SCOI\u306e\u7d0d\u54c1\u30c7\u30fc\u30bf\" width=\"672\" height=\"93\" srcset=\"https:\/\/gikenbio.com\/2025test\/wp-content\/uploads\/2022\/05\/nohin.jpg 788w, https:\/\/gikenbio.com\/2025test\/wp-content\/uploads\/2022\/05\/nohin-300x41.jpg 300w, https:\/\/gikenbio.com\/2025test\/wp-content\/uploads\/2022\/05\/nohin-768x106.jpg 768w\" sizes=\"auto, (max-width: 672px) 100vw, 672px\" \/><br \/>\n\u2605\u30c7\u30e2\u30c7\u30fc\u30bf\u3092\u3054\u89a7\u3044\u305f\u3060\u3051\u307e\u3059\uff08<a href=\"https:\/\/gikenbio.com\/2025test\/pdf\/otu_table_top10_river.xlsx\">MiFish<\/a>\u30fb<a href=\"https:\/\/gikenbio.com\/2025test\/pdf\/Mammal.xlsx\">MiMammal<\/a>\u30fb<a href=\"https:\/\/gikenbio.com\/2025test\/pdf\/Insect.xlsx\">gInsect<\/a>\u30fb<a href=\"https:\/\/gikenbio.com\/2025test\/pdf\/Animal.xlsx\">COI<\/a>\uff09<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center;\">\u2193<\/td>\n<td><\/td>\n<\/tr>\n<tr>\n<td class=\"flow\" rowspan=\"2\"><strong>\uff08\u30aa\u30d7\u30b7\u30e7\u30f3\uff09<br \/>\n\u7fa4\u9593\u6bd4\u8f03\u89e3\u6790<br \/>\n\u203b\u7d30\u83cc16S\/\u771f\u83ccITS\/18S\u306e\u307f\u5bfe\u5fdc<\/strong><\/td>\n<td width=\"371\">LEfSe\u3092\u4f7f\u3063\u3066\u6bd4\u8f03\u3059\u308b\u7fa4\u9593\u306e\u9055\u3044\u3068\u76f8\u95a2\u3092\u793a\u3059\u5fae\u751f\u7269\u7fa4\u306e\u62bd\u51fa\u3084\u3042\u308b\u73fe\u8c61\u306e\u539f\u56e0\u5fae\u751f\u7269\u306e\u63a2\u7d22\u3092\u884c\u3044\u307e\u3059\u3002<br \/>\n\u6709\u610f\u5dee\u691c\u5b9a\u3092\u884c\u3046\u305f\u3081\u3001\u89e3\u6790\u306b\u306f\u6700\u4f4e6\u30b5\u30f3\u30d7\u30eb \u304b\u3064 1\u30b0\u30eb\u30fc\u30d73\u30b5\u30f3\u30d7\u30eb\u4ee5\u4e0a\u5fc5\u8981\u3067\u3059\u3002<\/td>\n<\/tr>\n<tr>\n<td><a href=\"https:\/\/gikenbio.com\/2025test\/wp-content\/uploads\/2020\/07\/lefse.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-4356\" src=\"https:\/\/gikenbio.com\/2025test\/wp-content\/uploads\/2020\/07\/lefse.jpg\" alt=\"\" width=\"600\" height=\"275\" srcset=\"https:\/\/gikenbio.com\/2025test\/wp-content\/uploads\/2020\/07\/lefse.jpg 600w, https:\/\/gikenbio.com\/2025test\/wp-content\/uploads\/2020\/07\/lefse-300x138.jpg 300w\" sizes=\"auto, (max-width: 600px) 100vw, 600px\" \/><\/a><br \/>\n\u2605\u7fa4\u9593\u6bd4\u8f03\u89e3\u6790\u306e\u7d0d\u54c1\u30c7\u30fc\u30bf\u306e\u30b5\u30f3\u30d7\u30eb\u3092\u3054\u7528\u610f\u3057\u3066\u3044\u307e\u3059\u3002<a href=\"https:\/\/gikenbio.com\/2025test\/data\/LEfSE.zip\">\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u306f\u3053\u3061\u3089\u3002<\/a><\/td>\n<\/tr>\n<tr>\n<td class=\"flow\" rowspan=\"2\" width=\"25%\"><strong>\uff08\u30aa\u30d7\u30b7\u30e7\u30f3\uff09<br \/>\n\u591a\u69d8\u6027\u89e3\u6790<\/strong><\/td>\n<td>\u30ec\u30a2\u30d5\u30a1\u30af\u30b7\u30e7\u30f3\u89e3\u6790(chao1\u3084shannon\u306a\u3069)\u3068\u4e3b\u5ea7\u6a19\u5206\u6790 (PCoA)\u306e\u7d50\u679c\u3092\u63d0\u4f9b\u3044\u305f\u3057\u307e\u3059\u3002\u30b5\u30f3\u30d7\u30eb\u306e\u30b0\u30eb\u30fc\u30d7\u5206\u3051\u3092\u3054\u6307\u793a\u3044\u305f\u3060\u304f\u3053\u3068\u3067\u3001\u30b0\u30eb\u30fc\u30d7\u5358\u4f4d\u3067\u306e\u6bd4\u8f03\u304c\u53ef\u80fd\u3067\u3059\u3002<\/td>\n<\/tr>\n<tr>\n<td><img loading=\"lazy\" decoding=\"async\" class=\"size-full wp-image-3695 alignnone\" src=\"https:\/\/gikenbio.com\/2025test\/wp-content\/uploads\/2020\/05\/b043071f84a69072776800ab4c37930e.jpg\" alt=\"\u591a\u69d8\u6027\u89e3\u6790\" width=\"341\" height=\"121\" srcset=\"https:\/\/gikenbio.com\/2025test\/wp-content\/uploads\/2020\/05\/b043071f84a69072776800ab4c37930e.jpg 341w, https:\/\/gikenbio.com\/2025test\/wp-content\/uploads\/2020\/05\/b043071f84a69072776800ab4c37930e-300x106.jpg 300w\" sizes=\"auto, (max-width: 341px) 100vw, 341px\" \/><br \/>\n\u2605\u30d6\u30e9\u30a6\u30b6\u4e0a\u3067\u30c7\u30e2\u30c7\u30fc\u30bf\u3092\u3054\u89a7\u3044\u305f\u3060\u3051\u307e\u3059\uff08<a href=\"https:\/\/gikenbio.com\/2025test\/sampledata\/qiime2\/Beta_diversity\/index.html\" target=\"_blank\" rel=\"noopener noreferrer\">\u4e3b\u5ea7\u6a19\u5206\u6790<\/a>\u30fb<a href=\"https:\/\/gikenbio.com\/2025test\/sampledata\/rarefaction\/rarefaction_plots.html\" target=\"_blank\" rel=\"noopener noreferrer\">\u30ec\u30a2\u30d5\u30a1\u30af\u30b7\u30e7\u30f3\u30ab\u30fc\u30d6<\/a>\uff09<\/td>\n<\/tr>\n<tr>\n<td class=\"flow\"><strong>\uff08\u30aa\u30d7\u30b7\u30e7\u30f3\uff09<br \/>\n\u2517 \u591a\u69d8\u6027\u89e3\u6790\u306e\u7d71\u8a08\u89e3\u6790<br \/>\n\u203b\u591a\u69d8\u6027\u89e3\u6790\u3092\u884c\u3046\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059<br \/>\n<\/strong><\/td>\n<td>\u30d9\u30fc\u30bf\u591a\u69d8\u6027\u306fPERMANOVA\u3067\u691c\u5b9a\u3057\u3001\u30a2\u30eb\u30d5\u30a1\u591a\u69d8\u6027\u306fKruskal-Wallis\u3067\u691c\u5b9a\u3057\u307e\u3059\u3002<br \/>\n\u4e3b\u6210\u5206\u5206\u6790(PCA)\u306e\u7d50\u679c\u3068\u305d\u306e\u7d50\u679c\u306b\u4fe1\u983c\u6955\u5186(95%)\u3092\u52a0\u3048\u305f\u4ee5\u4e0b\u306e\u56f3\u3082\u7d0d\u54c1\u3057\u307e\u3059\u3002<br \/>\n\u6709\u610f\u5dee\u691c\u5b9a\u3092\u884c\u3046\u305f\u3081\u3001\u89e3\u6790\u306b\u306f\u6700\u4f4e6\u30b5\u30f3\u30d7\u30eb \u304b\u3064 1\u30b0\u30eb\u30fc\u30d73\u30b5\u30f3\u30d7\u30eb\u4ee5\u4e0a\u5fc5\u8981\u3067\u3059\u3002<br \/>\n<img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-5931\" src=\"https:\/\/gikenbio.com\/2025test\/wp-content\/uploads\/2023\/04\/confidence_ellipse_PC1PC3.png\" alt=\"\" width=\"215\" height=\"151\" \/><\/td>\n<\/tr>\n<tr>\n<td class=\"flow\" rowspan=\"2\"><strong>\uff08\u30aa\u30d7\u30b7\u30e7\u30f3\uff09<br \/>\n\u6a5f\u80fd\u4e88\u6e2c\u89e3\u6790<br \/>\n\u203b\u7d30\u83cc16S\u306e\u307f\u5bfe\u5fdc<\/strong><\/td>\n<td>PICRUSt2\u3092\u4f7f\u3063\u3066\u4e88\u6e2c\u30e1\u30bf\u30b2\u30ce\u30e0\u89e3\u6790\u3092\u884c\u3044\u307e\u3059\u3002<\/td>\n<\/tr>\n<tr>\n<td><a href=\"https:\/\/gikenbio.com\/2025test\/wp-content\/uploads\/2020\/07\/kinoyosokuHP.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-4354\" src=\"https:\/\/gikenbio.com\/2025test\/wp-content\/uploads\/2020\/07\/kinoyosokuHP.jpg\" alt=\"\u6a5f\u80fd\u4e88\u6e2c\u89e3\u6790\u30c7\u30fc\u30bf\" width=\"599\" height=\"163\" srcset=\"https:\/\/gikenbio.com\/2025test\/wp-content\/uploads\/2020\/07\/kinoyosokuHP.jpg 599w, https:\/\/gikenbio.com\/2025test\/wp-content\/uploads\/2020\/07\/kinoyosokuHP-300x82.jpg 300w\" sizes=\"auto, (max-width: 599px) 100vw, 599px\" \/><\/a><br \/>\n\u2605\u6a5f\u80fd\u4e88\u6e2c\u89e3\u6790\uff0b\u7d71\u8a08\u89e3\u6790\u306e\u7d0d\u54c1\u30c7\u30fc\u30bf\u306e\u30b5\u30f3\u30d7\u30eb\u3092\u3054\u7528\u610f\u3057\u3066\u3044\u307e\u3059\u3002<a href=\"https:\/\/gikenbio.com\/2025test\/data\/kinoyosoku_2.zip\">\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u306f\u3053\u3061\u3089\u3002<\/a><\/td>\n<\/tr>\n<tr>\n<td class=\"flow\" rowspan=\"2\"><strong>\uff08\u30aa\u30d7\u30b7\u30e7\u30f3\uff09<br \/>\n\u2517 \u6a5f\u80fd\u4e88\u6e2c\u89e3\u6790\u306e\u7d71\u8a08\u89e3\u6790<br \/>\n\u203b\u7d30\u83cc16S\u306e\u307f\u5bfe\u5fdc<br \/>\n\u203b\u6a5f\u80fd\u4e88\u6e2c\u89e3\u6790\u3092\u884c\u3046\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059<br \/>\n<\/strong><\/td>\n<td>\u6a5f\u80fd\u4e88\u6e2c\u89e3\u6790\u306e\u7d50\u679c\u304b\u3089\u6bd4\u8f03\u3059\u308b\u30b0\u30eb\u30fc\u30d7\u9593\u3067\u7d71\u8a08\u7684\u306b\u512a\u4f4d\u306a\u5dee\u304c\u307f\u3089\u308c\u308b\u304b\u3092\u691c\u5b9a\u3057\u307e\u3059\u3002<br \/>\n\u6709\u610f\u5dee\u691c\u5b9a\u3092\u884c\u3046\u305f\u3081\u3001\u89e3\u6790\u306b\u306f\u6700\u4f4e6\u30b5\u30f3\u30d7\u30eb \u304b\u3064 1\u30b0\u30eb\u30fc\u30d73\u30b5\u30f3\u30d7\u30eb\u4ee5\u4e0a\u5fc5\u8981\u3067\u3059\u3002<\/td>\n<\/tr>\n<tr>\n<td><a href=\"https:\/\/gikenbio.com\/2025test\/wp-content\/uploads\/2020\/07\/stampHP.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-4355\" src=\"https:\/\/gikenbio.com\/2025test\/wp-content\/uploads\/2020\/07\/stampHP.jpg\" alt=\"\" width=\"406\" height=\"242\" srcset=\"https:\/\/gikenbio.com\/2025test\/wp-content\/uploads\/2020\/07\/stampHP.jpg 513w, https:\/\/gikenbio.com\/2025test\/wp-content\/uploads\/2020\/07\/stampHP-300x179.jpg 300w\" sizes=\"auto, (max-width: 406px) 100vw, 406px\" \/><\/a><br \/>\n\u2605\u6a5f\u80fd\u4e88\u6e2c\u89e3\u6790\uff0b\u7d71\u8a08\u89e3\u6790\u306e\u7d0d\u54c1\u30c7\u30fc\u30bf\u306e\u30b5\u30f3\u30d7\u30eb\u3092\u3054\u7528\u610f\u3057\u3066\u3044\u307e\u3059\u3002<a href=\"https:\/\/gikenbio.com\/2025test\/data\/kinoyosoku_2.zip\">\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u306f\u3053\u3061\u3089\u3002<\/a><\/td>\n<\/tr>\n<tr>\n<td class=\"flow\"><strong>\uff08\u30aa\u30d7\u30b7\u30e7\u30f3\uff09<br \/>\n\u30b3\u30d4\u30fc\u6570\u306e\u53d6\u5f97<br \/>\n<\/strong><\/td>\n<td>\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u89e3\u6790\u3092\u884c\u3044\u3001\u30b5\u30f3\u30d7\u30eb\u4e2d\u306e\u5bfe\u8c61\u907a\u4f1d\u5b50\u306e\u30b3\u30d4\u30fc\u6570\u3092\u6e2c\u5b9a\u3057\u307e\u3059\u3002\u30a2\u30f3\u30d7\u30ea\u30b3\u30f3\u30b7\u30fc\u30b1\u30f3\u30b9\u89e3\u6790\u306e\u7d50\u679c\u3092\u76f8\u5bfe\u5024\u304b\u3089\u7d76\u5bfe\u5024\u306b\u5909\u63db\u3057\u305f\u30c7\u30fc\u30bf\u3092\u304a\u8fd4\u3057\u3057\u307e\u3059\u3002<\/td>\n<\/tr>\n<tr>\n<td class=\"flow\"><strong>\uff08\u30aa\u30d7\u30b7\u30e7\u30f3\uff09<br \/>\n\u30cd\u30c3\u30c8\u30ef\u30fc\u30af\u89e3\u6790<br \/>\n<span style=\"color: red;\">\u26052023.05 New\u2605<\/span><br \/>\n<\/strong><\/td>\n<td>SpiecEasi (MB\u6cd5) \u3092\u4f7f\u7528\u3057\u3066\u3001<a href=\"https:\/\/gikenbio.com\/2025test\/look-up\/network\/\" target=\"_blank\" rel=\"noopener\">\u30cd\u30c3\u30c8\u30ef\u30fc\u30af\u89e3\u6790<\/a>\u3092\u884c\u3044\u307e\u3059\u3002\u7d4c\u9a13\u4e0a\u3001\u6700\u4f4e8\u30b5\u30f3\u30d7\u30eb\u5fc5\u8981\u3067\u3059\u3002<br \/>\n<img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-5926\" src=\"https:\/\/gikenbio.com\/2025test\/wp-content\/uploads\/2023\/04\/MB.png\" alt=\"\" width=\"165\" height=\"165\" srcset=\"https:\/\/gikenbio.com\/2025test\/wp-content\/uploads\/2023\/04\/MB.png 480w, https:\/\/gikenbio.com\/2025test\/wp-content\/uploads\/2023\/04\/MB-300x300.png 300w, https:\/\/gikenbio.com\/2025test\/wp-content\/uploads\/2023\/04\/MB-150x150.png 150w, https:\/\/gikenbio.com\/2025test\/wp-content\/uploads\/2023\/04\/MB-250x250.png 250w\" sizes=\"auto, (max-width: 165px) 100vw, 165px\" \/><br \/>\n\u2605\u30cd\u30c3\u30c8\u30ef\u30fc\u30af\u89e3\u6790\u306e\u7d0d\u54c1\u30c7\u30fc\u30bf\u306e\u30b5\u30f3\u30d7\u30eb\u3092\u3054\u7528\u610f\u3057\u3066\u3044\u307e\u3059\u3002<a href=\"https:\/\/gikenbio.com\/2025test\/data\/network.zip\">\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u306f\u3053\u3061\u3089\u3002<\/a><\/td>\n<\/tr>\n<tr>\n<td class=\"flow\"><strong>\uff08\u30aa\u30d7\u30b7\u30e7\u30f3\uff09<br \/>\n\u751f\u606f\u74b0\u5883\u306e\u63a8\u6e2c<br \/>\n\u203b\u7d30\u83cc16S\u306e\u307f\u5bfe\u5fdc<\/strong><\/td>\n<td>16S rRNA\u907a\u4f1d\u5b50\u914d\u5217\u3068\u5fae\u751f\u7269\u306e\u751f\u606f\u74b0\u5883\u3092\u7db2\u7f85\u7684\u306b\u7d50\u3073\u3064\u3051\u308b\u30c7\u30fc\u30bf\u30d9\u30fc\u30b9\u300cProkAtlas\u300d\u3092\u7528\u3044\u3066\u3001\u751f\u606f\u74b0\u5883\u3092\u63a8\u6e2c\u3057\u307e\u3059\u3002<br \/>\n\u2605\u751f\u606f\u74b0\u5883\u306e\u63a8\u6e2c\u306e\u7d0d\u54c1\u30c7\u30fc\u30bf\u306e\u30b5\u30f3\u30d7\u30eb\u3092\u3054\u7528\u610f\u3057\u3066\u3044\u307e\u3059\u3002<a href=\"https:\/\/gikenbio.com\/2025test\/data\/habitat-result.xlsx\">\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u306f\u3053\u3061\u3089\u3002<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table><\/div>\n<div>\n<h3 class=\"style3b\">3.\u6599\u91d1\u65e9\u898b\u8868\uff08\u7a0e\u5225\uff09<\/h3>\n<div class=\"s_table\"><table>\n<tbody>\n<tr>\n<th width=\"16%\">\u30b5\u30f3\u30d7\u30eb\u6570<\/th>\n<th width=\"28%\"><strong>\u8a66\u6599\u3092\u9001\u308b<br \/>\n<\/strong><\/th>\n<th width=\"28%\"><strong>\u62bd\u51faDNA\u3092\u9001\u308b<\/strong><strong><br \/>\n<\/strong><\/th>\n<th width=\"28%\"><strong> 1stPCR\u7523\u7269\uff08\u7cbe\u88fd\u524d\uff09\u3092\u9001\u308b<\/strong><strong><br \/>\n<\/strong><\/th>\n<\/tr>\n<tr>\n<td>1<\/td>\n<td>20,000\u5186(@20,000\u5186\uff09<\/td>\n<td>15,000\u5186(@15,000\u5186\uff09<\/td>\n<td>15,000\u5186*1(@15,000\u5186\uff09<\/td>\n<\/tr>\n<tr>\n<td>8<\/td>\n<td>120,000\u5186(@15,000\u5186\uff09<\/td>\n<td>96,000\u5186(@12,000\u5186\uff09<\/td>\n<td>72,000\u5186*1(@9,000\u5186\uff09<\/td>\n<\/tr>\n<tr>\n<td>16<\/td>\n<td>240,000\u5186(@15,000\u5186\uff09<\/td>\n<td>192,000\u5186(@12,000\u5186\uff09<\/td>\n<td>144,000\u5186*1(@9,000\u5186\uff09<\/td>\n<\/tr>\n<tr>\n<td>24<\/td>\n<td>360,000\u5186(@15,000\u5186\uff09<\/td>\n<td>288,000\u5186(@12,000\u5186\uff09<\/td>\n<td>216,000\u5186*1(@9,000\u5186\uff09<\/td>\n<\/tr>\n<tr>\n<td>32<\/td>\n<td>480,000\u5186(@15,000\u5186\uff09<\/td>\n<td>384,000\u5186(@12,000\u5186\uff09<\/td>\n<td>288,000\u5186*1(@9,000\u5186\uff09<\/td>\n<\/tr>\n<tr>\n<td>40<\/td>\n<td>540,000\u5186(@13,500\u5186\uff09<\/td>\n<td>420,000\u5186(@10,500\u5186\uff09<\/td>\n<td>360,000\u5186*1(@9,000\u5186\uff09<\/td>\n<\/tr>\n<tr>\n<td>48<\/td>\n<td>576,000\u5186(@12,000\u5186\uff09<\/td>\n<td>432,000\u5186(@9,000\u5186\uff09<\/td>\n<td>384,000\u5186*1(@8,000\u5186\uff09<\/td>\n<\/tr>\n<tr>\n<td>56<\/td>\n<td>616,000\u5186(@11,000\u5186\uff09<\/td>\n<td>448,000\u5186(@8,000\u5186\uff09<\/td>\n<td>392,000\u5186*1(@7,000\u5186\uff09<\/td>\n<\/tr>\n<tr>\n<td>64<\/td>\n<td>672,000\u5186(@10,500\u5186\uff09<\/td>\n<td>480,000\u5186(@7,500\u5186\uff09<\/td>\n<td>416,000\u5186*1(@6,500\u5186\uff09<\/td>\n<\/tr>\n<tr>\n<td>72<\/td>\n<td>720,000\u5186(@10,000\u5186\uff09<\/td>\n<td>504,000\u5186(@7,000\u5186\uff09<\/td>\n<td>432,000\u5186*1(@6,000\u5186\uff09<\/td>\n<\/tr>\n<tr>\n<td>73\uff5e<\/td>\n<td>\uff2010,000\u5186<\/td>\n<td>@7,000\u5186<\/td>\n<td>\uff206,000\u5186*1<\/td>\n<\/tr>\n<\/tbody>\n<\/table><\/div>\n<p>*1\u30001stPCR\u7528\u306e\u30d7\u30e9\u30a4\u30de\u30fc\u9001\u4ed8\u6599\u91d1\uff081,500\u5186\/\u4f9d\u983c\uff09\u304c\u5225\u9014\u304b\u304b\u308a\u307e\u3059\u3002<\/p>\n<p class=\"well\"><strong><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-5433 alignleft\" src=\"https:\/\/gikenbio.com\/2025test\/wp-content\/uploads\/2022\/05\/d5c2fb51bee4ff12b7dc5ba6cf3b755e-162x300.png\" alt=\"\u3084\u304e\u6751\u3055\u3093\" width=\"41\" height=\"76\" srcset=\"https:\/\/gikenbio.com\/2025test\/wp-content\/uploads\/2022\/05\/d5c2fb51bee4ff12b7dc5ba6cf3b755e-162x300.png 162w, https:\/\/gikenbio.com\/2025test\/wp-content\/uploads\/2022\/05\/d5c2fb51bee4ff12b7dc5ba6cf3b755e.png 512w\" sizes=\"auto, (max-width: 41px) 100vw, 41px\" \/>\u30b5\u30f3\u30d7\u30eb\u6570\u304c8\u306e\u500d\u6570\u3067\u306a\u3044\u3068\u304d\u3082\u3001\u4e0a\u8a181\u30b5\u30f3\u30d7\u30eb\u3042\u305f\u308a\u306e\u5358\u4fa1\u3067\u5bfe\u5fdc\u3057\u307e\u3059\u3002<\/strong><br \/>\n\u4f8b\uff09\u8a66\u6599\u309218\u30b5\u30f3\u30d7\u30eb\u9001\u308b\u5834\u5408<br \/>\n16\u30b5\u30f3\u30d7\u30eb\u306e\u5834\u5408\u306e\u5358\u4fa1\u306f@15,000\u5186\u3067\u3059\u306e\u3067\u300115,000\u5186\u00d718\u30b5\u30f3\u30d7\u30eb=270,000\u5186\uff08\u7a0e\u5225\uff09\u3068\u306a\u308a\u307e\u3059\u3002<\/p>\n<p>&nbsp;<\/p>\n<div class=\"s_table\"><table style=\"width: 430px;\">\n<tbody>\n<tr>\n<th width=\"16%\">\u30b5\u30f3\u30d7\u30eb\u6570<\/th>\n<th width=\"84%\"><strong>2ndPCR\u7523\u7269\uff08\u7cbe\u88fd\u5f8c\u30fb\u6df7\u5408\u4e0d\u8981\uff09\u3092\u9001\u308b*2<br \/>\n<\/strong><\/th>\n<\/tr>\n<tr>\n<td>1\uff5e32<\/td>\n<td>\u53d7\u3051\u5165\u308c\u53ef\u80fd\u3067\u3059\u304c\u3001\u4fa1\u683c\u306f\u300c1stPCR\u7523\u7269\u3092\u9001\u308b\u300d\u3068\u540c\u3058\u3067\u3059\u3002<\/td>\n<\/tr>\n<tr>\n<td>33\uff5e96<\/td>\n<td>\u30d1\u30c3\u30b1\u30fc\u30b8\u3067\u306f\u306a\u304f\u3001\u4e0b\u8a18\u9805\u76ee\u3092\u8db3\u3057\u5408\u308f\u305b\u305f\u4fa1\u683c\u306b\u306a\u308a\u307e\u3059\u3002<br \/>\n\u30fb\u30b7\u30fc\u30b1\u30f3\u30b7\u30f3\u30b0\u89e3\u6790\u3000300,000\u5186\/\u5f0f<br \/>\n\u30fb\u30c7\u30fc\u30bf\u89e3\u6790\u3000500\u5186\/\u30b5\u30f3\u30d7\u30eb<br \/>\n\u30fb\u30d7\u30e9\u30a4\u30de\u30fc\u9001\u4ed8\u300010,000\u5186\/\u56de<br \/>\n\u4f8b\uff0940\u30b5\u30f3\u30d7\u30eb\u30fb\u30c7\u30fc\u30bf\u89e3\u6790\u3042\u308a\u306e\u5834\u5408\uff1a300,000+500\u00d740+10,000=330,000\u5186<\/p>\n<p class=\"well\">\u25c6\u30b7\u30fc\u30b1\u30f3\u30b7\u30f3\u30b0\u89e3\u6790\u306e\u6761\u4ef6<br \/>\nMiSeq\u307e\u305f\u306fNextSeq 1000\u3092\u7528\u3044\u3066\u30012\u00d7300 bp\u306e\u6761\u4ef6\u3067\u89e3\u6790\u3057\u307e\u3059\u3002<br \/>\n\u25c6\u30c7\u30fc\u30bf\u91cf<br \/>\n\u30c8\u30fc\u30bf\u30eb300-400\u4e07\u30ea\u30fc\u30c9\u30da\u30a2\u7a0b\u5ea6\u3067\u3059\u3002<br \/>\n\u25c6\u7d0d\u671f<br \/>\n12\u55b6\u696d\u65e5\uff08\u4f46\u3057\u3001\u4ed6\u306e\u304a\u5ba2\u69d8\u3068\u306e\u30a4\u30f3\u30c7\u30c3\u30af\u30b9\u306e\u91cd\u8907\u304c\u3042\u308b\u5834\u5408\u30015\u55b6\u696d\u65e5\u7a0b\u5ea6\u8ffd\u52a0\u306b\u306a\u308a\u307e\u3059\uff09<\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table><\/div>\n<p>*2\u3000\u9001\u4ed8\u3059\u308b\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u306e\u6fc3\u5ea6\u3068\u6db2\u91cf\u306f\u300110ng\/\u03bcl\u4ee5\u4e0a\u300120\u03bcl \u4ee5\u4e0a\u3067\u304a\u9858\u3044\u3057\u307e\u3059\u3002\u7cbe\u88fd\u6e08\u306e\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u3092\u30d7\u30ec\u30fc\u30c8\uff08\u307e\u305f\u306f8\u9023\u30c1\u30e5\u30fc\u30d6\uff09\u306b\u5165\u308c\u3066\u51b7\u8535\u4fbf or \u51b7\u51cd\u4fbf\u3067\u9001\u4ed8\u3057\u3066\u304f\u3060\u3055\u3044\u3002<\/p>\n<h3 class=\"style3b\">4.\u30aa\u30d7\u30b7\u30e7\u30f3\u6599\u91d1\u8868<\/h3>\n<div class=\"s_table\"><table style=\"height: 756px;\">\n<tbody>\n<tr style=\"height: 30px;\">\n<td style=\"height: 30px;\" bgcolor=\"#eee\">\u9805\u76ee<\/td>\n<td style=\"height: 30px;\" bgcolor=\"#eee\">\u4fa1\u683c\uff08\u7a0e\u5225\uff09<\/td>\n<\/tr>\n<tr style=\"height: 30px;\">\n<td style=\"height: 30px;\">\u6db2\u4f53\u30b5\u30f3\u30d7\u30eb\u306e\u30d5\u30a3\u30eb\u30bf\u30fc\u308d\u904e<\/td>\n<td style=\"height: 30px;\">4,000\u5186\/\u30b5\u30f3\u30d7\u30eb<\/td>\n<\/tr>\n<tr style=\"height: 30px;\">\n<td style=\"height: 30px;\">PCR\u963b\u5bb3\u7269\u8cea\u306e\u9664\u53bb\uff08\u30ab\u30e9\u30e0\u7cbe\u88fd\uff09<\/td>\n<td style=\"height: 30px;\">2,000\u5186\/\u30b5\u30f3\u30d7\u30eb<\/td>\n<\/tr>\n<tr style=\"height: 30px;\">\n<td style=\"height: 30px;\">1stPCR 4\u53cd\u5fa9<\/td>\n<td style=\"height: 30px;\">16,000\u5186\/8\u30b5\u30f3\u30d7\u30eb<\/td>\n<\/tr>\n<tr style=\"height: 30px;\">\n<td style=\"height: 30px;\">\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u306e\u30d3\u30fc\u30ba\u7cbe\u88fd\uff08\u6ce84\uff09<\/td>\n<td style=\"height: 30px;\">5,000\u5186\/8\u30b5\u30f3\u30d7\u30eb<\/td>\n<\/tr>\n<tr style=\"height: 30px;\">\n<td style=\"height: 30px;\">\u30d6\u30ed\u30c3\u30ad\u30f3\u30b0<\/td>\n<td style=\"height: 30px;\"><a href=\"https:\/\/gikenbio.com\/2025test\/dnaanalysis\/ngs\/amplicon\/blocking\/\">\u3053\u3061\u3089\u3092\u3054\u89a7\u304f\u3060\u3055\u3044<\/a><\/td>\n<\/tr>\n<tr style=\"height: 30px;\">\n<td style=\"height: 30px;\">\u6b8b\u30b5\u30f3\u30d7\u30eb\u306e\u8fd4\u5374\u3000\u203b\u9001\u6599\u8fbc<\/td>\n<td style=\"height: 30px;\">2,500\u5186\/\u56de\u3000<span style=\"color: red;\">\u26052024.05 \u5024\u4e0a\u3052\u2605<\/span><\/td>\n<\/tr>\n<tr style=\"height: 30px;\">\n<td style=\"height: 30px;\">QIIME1\u306b\u5909\u66f4<\/td>\n<td style=\"height: 30px;\">0\u5186<\/td>\n<\/tr>\n<tr style=\"height: 30px;\">\n<td style=\"height: 30px;\">EzBioCloud 16S database\u4f7f\u7528\uff08<a href=\"https:\/\/gikenbio.com\/2025test\/2019\/05\/19\/%e7%b4%b0%e8%8f%8c%e5%8f%a2%e3%81%ae%e7%b3%bb%e7%b5%b1%e6%8e%a8%e5%ae%9a%e7%b5%90%e6%9e%9c%e3%82%92%e5%b1%9e%e3%83%ac%e3%83%99%e3%83%ab%e3%81%8b%e3%82%89%e7%a8%ae%e3%83%ac%e3%83%99%e3%83%ab%e3%81%ab\/\" target=\"_blank\" rel=\"noopener noreferrer\">\u8a73\u3057\u304f\u306f\u3053\u3061\u3089<\/a>\uff09<\/td>\n<td style=\"height: 30px;\">10,000\u5186\/\u89e3\u6790<\/td>\n<\/tr>\n<tr style=\"height: 61px;\">\n<td style=\"height: 61px;\">\u7fa4\u9593\u6bd4\u8f03\u89e3\u6790\uff08\u7d30\u83cc16S\/\u771f\u83ccITS\/18S\u306e\u307f\u53ef\u80fd\uff09<\/td>\n<td style=\"height: 61px;\">1\u6bd4\u8f03\u76ee 30,000\u5186<br \/>\n\u203b\u8ffd\u52a01\u6bd4\u8f03\u3054\u306810,000\u5186<\/td>\n<\/tr>\n<tr style=\"height: 30px;\">\n<td style=\"height: 30px;\">\u591a\u69d8\u6027\u89e3\u6790<\/td>\n<td style=\"height: 30px;\">10,000\u5186\/\u89e3\u6790<\/td>\n<\/tr>\n<tr style=\"height: 30px;\">\n<td style=\"height: 30px;\">\u2517 \u591a\u69d8\u6027\u89e3\u6790\u306e\u7d71\u8a08\u89e3\u6790\uff08\u591a\u69d8\u6027\u89e3\u6790\u306e\u30aa\u30d7\u30b7\u30e7\u30f3\uff09<\/td>\n<td style=\"height: 30px;\">20,000\u5186\/\u89e3\u6790<br \/>\n\u203b\u8907\u6570\u9818\u57df\u306e\u3054\u6ce8\u6587\u6642\u306a\u30692\u89e3\u6790\u76ee\u306f10,000\u5186\/\u89e3\u6790<\/td>\n<\/tr>\n<tr style=\"height: 30px;\">\n<td style=\"height: 30px;\">\u6a5f\u80fd\u4e88\u6e2c\u89e3\u6790\uff08\u7d30\u83cc16S\u306e\u307f\u53ef\u80fd\uff09<\/td>\n<td style=\"height: 30px;\">30,000\u5186\/\u89e3\u6790<\/td>\n<\/tr>\n<tr style=\"height: 61px;\">\n<td style=\"height: 61px;\">\u2517 \u6a5f\u80fd\u4e88\u6e2c\u89e3\u6790\u306e\u7d71\u8a08\u89e3\u6790\uff08\u7d30\u83cc16S\u306e\u307f\u53ef\u80fd\/\u6a5f\u80fd\u4e88\u6e2c\u89e3\u6790\u306e\u30aa\u30d7\u30b7\u30e7\u30f3\uff09<\/td>\n<td style=\"height: 61px;\">1\u6bd4\u8f03\u76ee 30,000\u5186<br \/>\n\u203b\u8ffd\u52a01\u6bd4\u8f03\u3054\u306810,000\u5186<\/td>\n<\/tr>\n<tr style=\"height: 30px;\">\n<td style=\"height: 30px;\">\u6a5f\u80fd\u4e88\u6e2c\u89e3\u6790\u3068\u7fa4\u9593\u6bd4\u8f03\u89e3\u6790\u306e\u30bb\u30c3\u30c8\u5272\u5f15<\/td>\n<td style=\"height: 30px;\">3\u5272\u5f15<\/td>\n<\/tr>\n<tr style=\"height: 92px;\">\n<td style=\"height: 184px;\" rowspan=\"2\">\u30b3\u30d4\u30fc\u6570\u306e\u53d6\u5f97(\u6ce85)<\/td>\n<td style=\"height: 92px;\">\uff1c\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u89e3\u6790\u30921\u53cd\u5fa9\u5b9f\u65bd\u3059\u308b\u5834\u5408\uff1e<br \/>\n\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u89e3\u6790\u30005,000\u5186\/\u30b5\u30f3\u30d7\u30eb<br \/>\n\u691c\u91cf\u7dda\u4f5c\u6210\u300020,000\u5186\uff0880\u30b5\u30f3\u30d7\u30eb\u3054\u3068\uff09<\/td>\n<\/tr>\n<tr style=\"height: 92px;\">\n<td style=\"height: 92px;\">\uff1c\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u89e3\u6790\u30924\u53cd\u5fa9\u5b9f\u65bd\u3059\u308b\u5834\u5408\uff1e<br \/>\n\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u89e3\u6790\u30008,000\u5186\/\u30b5\u30f3\u30d7\u30eb<br \/>\n\u691c\u91cf\u7dda\u4f5c\u6210\u300020,000\u5186\uff0820\u30b5\u30f3\u30d7\u30eb\u3054\u3068\uff09<\/td>\n<\/tr>\n<tr style=\"height: 30px;\">\n<td style=\"height: 30px;\">\u30cd\u30c3\u30c8\u30ef\u30fc\u30af\u89e3\u6790<\/td>\n<td style=\"height: 30px;\">50,000\u5186\/\u89e3\u6790<\/td>\n<\/tr>\n<tr style=\"height: 30px;\">\n<td style=\"height: 30px;\">\u751f\u606f\u74b0\u5883\u306e\u63a8\u6e2c\uff08\u7d30\u83cc16S\u306e\u307f\u53ef\u80fd\uff09<\/td>\n<td style=\"height: 30px;\">20,000\u5186\/\u89e3\u6790<\/td>\n<\/tr>\n<\/tbody>\n<\/table><\/div>\n<p>(\u6ce84) \u9001\u4ed8\u3044\u305f\u3060\u3044\u305f\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u304c\u7cbe\u88fd\u4e0d\u8db3\u306e\u5834\u5408\u3001\u5f0a\u793e\u3067\u518d\u7cbe\u88fd\u3092\u884c\u3044\u307e\u3059\u3002<br \/>\n(\u6ce85) \u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u89e3\u6790\u3068\u691c\u91cf\u7dda\u4f5c\u6210\u306e\u4e21\u65b9\u304c\u5fc5\u8981\u3067\u3059\u3002\u307e\u305f\u3001\u30a2\u30f3\u30d7\u30ea\u30b3\u30f3\u30b7\u30fc\u30b1\u30f3\u30b9\u89e3\u6790\u3068\u540c\u6642\u306b\u3054\u6ce8\u6587\u3044\u305f\u3060\u3044\u305f\u969b\u306e\u4fa1\u683c\u3067\u3059\u3002<\/p>\n<div>\n<h3 class=\"style3b\">5.\u5bfe\u8c61\u907a\u4f1d\u5b50\u9818\u57df<\/h3>\n<div class=\"s_table\"><table>\n<tbody>\n<tr>\n<td bgcolor=\"#eee\">\u30bf\u30fc\u30b2\u30c3\u30c8\u751f\u7269\u7a2e<\/td>\n<td bgcolor=\"#eee\">\u5bfe\u8c61\u9818\u57df<\/td>\n<td bgcolor=\"#eee\">\u7d0d\u54c1\u30c7\u30fc\u30bf<\/td>\n<\/tr>\n<tr>\n<td rowspan=\"2\">\u7d30\u83cc\u3068\u53e4\u7d30\u83cc<\/td>\n<td>16S rRNA V4\uff1a515F-806R<\/td>\n<td rowspan=\"8\">\u53ef\u8996\u5316\u30c7\u30fc\u30bf\u3092\u7d0d\u54c1<\/td>\n<\/tr>\n<tr>\n<td>16S rRNA V4\uff08ver2\uff09\uff1a515F-806RB<br \/>\n<span style=\"color: red;\">\u26052023.05 New\u2605 \u4e0a\u8a18515F-806R\u306e\u6539\u826f\u7248\u3067\u3059\u3002\u6d77\u6d0b\u8868\u5c64\u4e2d\u306e\u81ea\u7531\u751f\u6d3b\u6027\u7d30\u83cc\u3067\u3042\u308bSAR11\u30af\u30ec\u30fc\u30c9\u306eDNA\u3092\u691c\u51fa\u3055\u308c\u3084\u3059\u304f\u6539\u5909\u3055\u308c\u307e\u3057\u305f\u3002<\/span><\/td>\n<\/tr>\n<tr>\n<td>\u7d30\u83cc<\/td>\n<td>16S rRNA V3\/V4\uff1a341f-805r<br \/>\n16S rRNA V1\/V2\uff1a27Fmod-338R<\/td>\n<\/tr>\n<tr>\n<td rowspan=\"3\">\u771f\u83cc\u985e<\/td>\n<td>ITS1\uff1aITS1F_KYO1 &#8211; ITS2_KYO2<br \/>\n\uff08\u690d\u7269\u7531\u6765DNA\u306f\u5897\u5e45\u3057\u306b\u304f\u3044\uff09<\/td>\n<\/tr>\n<tr>\n<td>ITS2\uff1agITS7 &#8211; ITS4<br \/>\n(\u771f\u83cc\u985e\u3092\u5e83\u304f\u30ab\u30d0\u30fc\u3057\u3066\u3044\u308b\u304c\u3001\u690d\u7269\u7531\u6765DNA\u304c\u5897\u5e45\u3059\u308b)<\/td>\n<\/tr>\n<tr>\n<td>ITS2\uff1afITS7 &#8211; ITS4<br \/>\n(gITS\u307b\u3069\u771f\u83cc\u985e\u3092\u5e83\u304f\u30ab\u30d0\u30fc\u3057\u3066\u3044\u306a\u3044\u304c\u3001\u690d\u7269\u7531\u6765DNA\u306f\u5897\u5e45\u3057\u306b\u304f\u3044)<\/td>\n<\/tr>\n<tr>\n<td rowspan=\"2\">\u771f\u6838\u751f\u7269<\/td>\n<td>18S rRNA\uff1a1422f-1642r<\/td>\n<\/tr>\n<tr>\n<td>18S rRNA ver2\uff1aV8f-1510r<br \/>\n<span style=\"color: red;\">\u26052025.05 New\u2605 \u6700\u8fd1\u306e\u8ad6\u6587\u3067\u591a\u304f\u4f7f\u308f\u308c\u3066\u3044\u308b\u30d7\u30e9\u30a4\u30de\u30fc\u3067\u3059\u3002<\/span><\/td>\n<\/tr>\n<tr>\n<td>\u52d5\u7269<\/td>\n<td>COI\uff1aIntF-HCOmR<\/td>\n<td rowspan=\"15\">\u30a8\u30af\u30bb\u30eb\u30c7\u30fc\u30bf\u3092\u7d0d\u54c1<\/td>\n<\/tr>\n<tr>\n<td>\u9b5a\u985e\uff08\u203b1\uff09<\/td>\n<td>12S rRNA (cMiFish)\u3000\u30d0\u30fc\u30b8\u30e7\u30f3\u30a2\u30c3\u30d7<\/td>\n<\/tr>\n<tr>\n<td>\u54fa\u4e73\u985e\uff08\u203b2\uff09<\/td>\n<td>12S rRNA (MiMammal)<\/td>\n<\/tr>\n<tr>\n<td>\u7bc0\u8db3\u52d5\u7269\uff08\u203b3\uff09<\/td>\n<td>16S rRNA (gInsect)<\/td>\n<\/tr>\n<tr>\n<td>\u9ce5\u985e\uff08\u203b3\uff09\uff08\u203b4\uff09<\/td>\n<td>16S rRNA (gBird)<\/td>\n<\/tr>\n<tr>\n<td>\u4e8c\u679a\u8c9d\uff08\u203b3\uff09<\/td>\n<td>18S rRNA (gClam)<\/td>\n<\/tr>\n<tr>\n<td>\u5dfb\u8c9d\uff08\u203b3\uff09<\/td>\n<td>28S rRNA (gSnail)<\/td>\n<\/tr>\n<tr>\n<td>\u6709\u5c3e\u76ee\uff08\u30a4\u30e2\u30ea\u985e\u3068\u30b5\u30f3\u30b7\u30e7\u30a6\u30a6\u30aa\u985e\uff09\uff08\u203b3\uff09<\/td>\n<td>12S rRNA (gSalamander)<\/td>\n<\/tr>\n<tr>\n<td>\u7121\u5c3e\u76ee\uff08\u203b3\uff09<\/td>\n<td>16S rRNA (gFrog)<\/td>\n<\/tr>\n<tr>\n<td>\u74b0\u5f62\u52d5\u7269\uff08\u203b3\uff09<\/td>\n<td>16S rRNA (gWorm)<\/td>\n<\/tr>\n<tr>\n<td>\u7dda\u866b\uff08\u203b3\uff09\uff08\u203b5\uff09<\/td>\n<td>18S rRNA (Ecdysozoa)<\/td>\n<\/tr>\n<tr>\n<td>\u9678\u4e0a\u690d\u7269\uff08\u203b3\uff09<\/td>\n<td>rbcL\uff08gPlant\uff09<\/td>\n<\/tr>\n<tr>\n<td>\u5149\u5408\u6210\u751f\u7269\uff08\u4e3b\u306b\u5358\u7d30\u80de\u751f\u7269\uff09\uff08\u203b3\uff09<\/td>\n<td>psbA\uff1apsbA_597f-psbA_927r<\/td>\n<\/tr>\n<tr>\n<td>\u8910\u85fb\uff08\u203b3\uff09<\/td>\n<td>rbcL\uff08gKasso\uff09<\/td>\n<\/tr>\n<tr>\n<td>\u7d05\u85fb\uff08\u203b3\uff09<\/td>\n<td>rbcL\uff08gKoso\uff09<\/td>\n<\/tr>\n<tr valign=\"top\">\n<td>\u30ab\u30b9\u30bf\u30e0\uff08\u203b6\uff09<\/td>\n<td>\u4efb\u610f\u306e\u9818\u57df (120\uff5e500 bp)<br \/>\n\u203b\u500b\u5225\u5bfe\u5fdc\u3055\u305b\u3066\u3044\u305f\u3060\u304d\u307e\u3059\u3002<\/td>\n<td>\u203b\u9818\u57df\u306b\u3088\u308b<\/td>\n<\/tr>\n<\/tbody>\n<\/table><\/div>\n<p>\u203b1\u30002020\u5e745\u6708\u3088\u308a\u30d7\u30e9\u30a4\u30de\u30fc\u30bb\u30c3\u30c8\u306e\u540d\u79f0\u30fb\u6df7\u5408\u5185\u5bb9\u304c\u5909\u308f\u308a\u307e\u3059\u3002<br \/>\n\u65e7\uff09\u6de1\u6c34\u7528MiFish\u2192MiFish-U-F\/R\u3068MiFish-E-F\/R (Miya et al., 2015)\u30921:1\u3067\u6df7\u5408<br \/>\n\u6d77\u6c34\u7528MiFish\u2192MiFish-U-F\/R\u3068MiFish-E-F\/R-v2\u3001MiFish-U2-F\/R (unpublished primer)\u30922:1:1\u3067\u6df7\u5408<br \/>\n\u65b0\uff09cMiFish\u2192MiFish-U-F\/R\u3068MiFish-E-F\/R-v2\u3001MiFish-U2-F\/R \u3001MiFish-Ayu&amp;Wakasagi(unpublished primer)\u30924:2:2:1\u3067\u6df7\u5408<br \/>\n\u203b2\u3000\u5f0a\u793e\u3067\u306e\u30c6\u30b9\u30c8\u89e3\u6790\u306e\u7d50\u679c\u3001\u810a\u690e\u52d5\u7269\u5168\u822c\u304c\u691c\u51fa\u3055\u308c\u3066\u3044\u307e\u3059\u3002<br \/>\n\u203b3\u3000\u81ea\u793e\u3067\u958b\u767a\u3057\u305f\u30d7\u30e9\u30a4\u30de\u30fc\u3067\u306e\u89e3\u6790\u3067\u3059\u3002<br \/>\n\u203b4\u3000\u9ce5\u985e\u306f\u74b0\u5883DNA\u3078\u306e\u79fb\u884c\u304c\u5c11\u306a\u304f\u3001\u74b0\u5883\u30b5\u30f3\u30d7\u30eb\u3067\u306f\u691c\u51fa\u304c\u56f0\u96e3\u306a\u50be\u5411\u306b\u3042\u308a\u307e\u3059\u3002<br \/>\n\u203b5\u3000\u690d\u7269\u7531\u6765DNA\u3082\u5897\u5e45\u3055\u308c\u307e\u3059\u3002<br \/>\n\u203b6\u3000\u30d7\u30e9\u30a4\u30de\u30fc\u306e\u65b0\u898f\u5408\u6210\u306f5,000\u5186\/2\u672c\u304b\u304b\u308a\u307e\u3059\u3002<\/p>\n<h3 class=\"style3b\">6.\u3088\u304f\u3042\u308b\u8cea\u554f<\/h3>\n<p><a href=\"https:\/\/gikenbio.com\/2025test\/faqs\/top\/amplicon_s\/\" target=\"_blank\" rel=\"noopener\">FAQs\u306e\u30da\u30fc\u30b8<\/a>\u3092\u3054\u89a7\u304f\u3060\u3055\u3044\u3002<\/p>\n<\/div>\n<div>\n<h3 class=\"style3b\">7.\u95a2\u9023\u30da\u30fc\u30b8<\/h3>\n<ul>\n<li><a href=\"https:\/\/gikenbio.com\/2025test\/dnaanalysis\/ngs\/amplicon\/quotation\/\">\u898b\u7a4d\u308a\u4f9d\u983c\u30d5\u30a9\u30fc\u30e0<\/a><\/li>\n<li><a href=\"https:\/\/gikenbio.com\/2025test\/dnaanalysis\/ngs\/amplicon\/techinfo\/\">\u30b5\u30f3\u30d7\u30eb\u8abf\u6574\u306b\u3064\u3044\u3066<\/a><\/li>\n<li><a href=\"https:\/\/gikenbio.com\/2025test\/dnaanalysis\/ngs\/amplicon\/blocking\/\">\uff1c\u5fdc\u7528\u7de8\uff1e\u30d6\u30ed\u30c3\u30ad\u30f3\u30b0<\/a><\/li>\n<\/ul>\n<h3 class=\"style3b\">8.\u3061\u3089\u3057<\/h3>\n<\/div>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-5520\" src=\"https:\/\/gikenbio.com\/2025test\/wp-content\/uploads\/2022\/05\/amplicon.jpg\" alt=\"\u7d30\u83cc\u53e2\u89e3\u6790\u3061\u3089\u3057\" width=\"126\" height=\"178\" srcset=\"https:\/\/gikenbio.com\/2025test\/wp-content\/uploads\/2022\/05\/amplicon.jpg 450w, https:\/\/gikenbio.com\/2025test\/wp-content\/uploads\/2022\/05\/amplicon-212x300.jpg 212w\" sizes=\"auto, (max-width: 126px) 100vw, 126px\" \/>\u3000<a class=\"q_button bt_green\" href=\"https:\/\/gikenbio.com\/2025test\/pdf\/amplicon.pdf\" target=\"_blank\" rel=\"noopener noreferrer\">\u30a2\u30f3\u30d7\u30ea\u30b3\u30f3\u30b7\u30fc\u30b1\u30f3\u30b9\u89e3\u6790\u306e\u30d1\u30f3\u30d5\u30ec\u30c3\u30c8PDF<\/a><\/p>\n<\/div>\n<h3 class=\"style3b\">9.\u304a\u554f\u5408\u305b\u30fb\u304a\u898b\u7a4d\u308a\u4f9d\u983c<\/h3>\n<div>\n<h4 class=\"style4b\">\u304a\u554f\u5408\u305b\u30fb\u304a\u898b\u7a4d\u308a\u4f9d\u983c<\/h4>\n<p><a href=\"https:\/\/gikenbio.com\/2025test\/dnaanalysis\/ngs\/amplicon\/quotation\/\" target=\"_blank\" rel=\"noopener noreferrer\">\u304a\u898b\u7a4d\u308a\u4f9d\u983c\u30d5\u30a9\u30fc\u30e0<\/a>\u3092\u3054\u7528\u610f\u3057\u3066\u3044\u307e\u3059\u3002\u307e\u305f\u3001<a href=\"https:\/\/gikenbio.com\/2025test\/contacts\/\">\u304a\u554f\u5408\u305b\u30d5\u30a9\u30fc\u30e0<\/a>\u3001\u30e1\u30fc\u30eb\uff08dna@gikenbio.com\uff09\u3001\u307e\u305f\u306f \u304a\u96fb\u8a71\uff08042-780-8333\uff09\u3067\u304a\u6c17\u8efd\u306b\u3054\u76f8\u8ac7\u304f\u3060\u3055\u3044\u3002<\/p>\n<h4 class=\"style4b\">\u30b5\u30f3\u30d7\u30eb\u9001\u4ed8\u5148<\/h4>\n<p>\u682a\u5f0f\u4f1a\u793e\u751f\u7269\u6280\u7814<br \/>\n\u3012252-0154\u3000\u795e\u5948\u5ddd\u770c\u76f8\u6a21\u539f\u5e02\u7dd1\u533a\u9577\u7af9657\u3000\/\u3000TEL 042-780-8333<br \/>\n\u203b\u5e73\u65e5\u7740\u3067\u304a\u9001\u308a\u304f\u3060\u3055\u3044\u3002\u767a\u9001\u306f\u5143\u6255\u3044\u3067\u304a\u9858\u3044\u3057\u3066\u3044\u307e\u3059\u3002<\/p>\n<\/div>\n","protected":false},"excerpt":{"rendered":"\u3053\u308c\u307e\u3067\u30b7\u30fc\u30b1\u30f3\u30b5\u30fc\u306fMiSeq\u306e\u307f\u3067\u3057\u305f\u304c\u3001 2024\u5e748\u6708\u9803\u3088\u308aNextSeq1000\u304c\u7a3c\u50cd\u3057\u307e\u3057\u305f\u3002 \u3044\u305a\u308c\u3082\u30012\u00d7300 bp\u306e\u6761\u4ef6\u3067\u30b7\u30fc\u30b1\u30f3\u30b7\u30f3\u30b0\u3092\u884c\u3044\u307e\u3059\u3002 \u672c\u89e3\u6790\u30b5\u30fc\u30d3\u30b9\u306e\u7279\u5fb4 \u30fb1\u30b5\u30f3\u30d7\u30eb\u3067\u3082\u3054\u6ce8\u6587\u53ef\u80fd\u3067 [&hellip;]","protected":false},"author":1,"featured_media":0,"parent":10,"menu_order":1,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-41","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/gikenbio.com\/2025test\/wp-json\/wp\/v2\/pages\/41","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/gikenbio.com\/2025test\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/gikenbio.com\/2025test\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/gikenbio.com\/2025test\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/gikenbio.com\/2025test\/wp-json\/wp\/v2\/comments?post=41"}],"version-history":[{"count":261,"href":"https:\/\/gikenbio.com\/2025test\/wp-json\/wp\/v2\/pages\/41\/revisions"}],"predecessor-version":[{"id":6893,"href":"https:\/\/gikenbio.com\/2025test\/wp-json\/wp\/v2\/pages\/41\/revisions\/6893"}],"up":[{"embeddable":true,"href":"https:\/\/gikenbio.com\/2025test\/wp-json\/wp\/v2\/pages\/10"}],"wp:attachment":[{"href":"https:\/\/gikenbio.com\/2025test\/wp-json\/wp\/v2\/media?parent=41"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}