{"id":5748,"date":"2022-10-06T08:27:46","date_gmt":"2022-10-05T23:27:46","guid":{"rendered":"https:\/\/gikenbio.com\/2025test\/?page_id=5748"},"modified":"2025-07-30T21:58:33","modified_gmt":"2025-07-30T12:58:33","slug":"isoseq","status":"publish","type":"page","link":"https:\/\/gikenbio.com\/2025test\/dnaanalysis\/ngs\/isoseq\/","title":{"rendered":"Iso-seq\u89e3\u6790\uff08\u30ed\u30f3\u30b0\u30ea\u30fc\u30c9RNA-seq\u89e3\u6790\u30fbPacBio\u793e\uff09"},"content":{"rendered":"\n

1.Iso-seq\u89e3\u6790\uff08\u30ed\u30f3\u30b0\u30ea\u30fc\u30c9RNA-seq\u89e3\u6790\u30fbPacBio\uff09\u30b5\u30fc\u30d3\u30b9\u6982\u8981<\/h3>\n
\n\n\n\n\n\n\n\n\n\n\n\n
\u5bfe\u8c61\u751f\u7269<\/th>\n\u771f\u6838\u751f\u7269\u306e\u307f\u3002
\ncDNA\u5408\u6210\u306boligo dT\u3092\u7528\u3044\u3066\u3044\u308b\u306e\u3067\u3001polyA\u3092\u6301\u305f\u306a\u3044\u751f\u7269\u306fLib\u8abf\u88fd\u3067\u304d\u305a\u89e3\u6790\u4e0d\u53ef\u3068\u306a\u308a\u307e\u3059\u3002<\/td>\n<\/tr>\n
\u4fa1\u683c\uff08\u7a0e\u5225\uff09<\/th>\n10\u4e07\u30ea\u30fc\u30c9\u3000150,000\u5186\/\u30b5\u30f3\u30d7\u30eb<\/span> \u26052025.05 \u304a\u8a66\u305710\u4e07\u30ea\u30fc\u30c9\u30d7\u30e9\u30f3\u767b\u5834\u2605<\/span><\/strong>
\n100\u4e07\u30ea\u30fc\u30c9\u3000420,000\u5186\/\u30b5\u30f3\u30d7\u30eb<\/span><\/strong>
\n200\u4e07\u30ea\u30fc\u30c9\u3000525,000\u5186\/\u30b5\u30f3\u30d7\u30eb<\/span><\/strong>
\n500\u4e07\u30ea\u30fc\u30c9\u7a0b\u5ea6\uff08\uff11\u30bb\u30eb\u5360\u6709\uff09\u203b1\u3000800,000\u5186\/\u30bb\u30eb<\/span><\/strong>
\n\u53d6\u5f97\u30c7\u30fc\u30bf\u91cf\u306f\u5168\u907a\u4f1d\u5b50\u6570\u306e20-50\u500d\u304c\u76ee\u5b89\u3067\u3059\u3002<\/td>\n<\/tr>\n
\u7d0d\u671f<\/th>\n30\u55b6\u696d\u65e5
\n\u203b3\u30bb\u30eb\u4ee5\u4e0a\u306e\u5834\u5408\u306f\u8981\u76f8\u8ac7<\/td>\n<\/tr>\n
\u4f5c\u696d\u5185\u5bb9<\/th>\n\u30fb\u9001\u4ed8\u30b5\u30f3\u30d7\u30eb\uff08\u30c8\u30fc\u30bf\u30ebRNA\uff09\u306e\u54c1\u8cea\u78ba\u8a8d
\n\u30fb\u30aa\u30ea\u30b4dT\u30d7\u30e9\u30a4\u30de\u30fc\u3067cDNA\u5408\u6210
\n\u30fb\u30b7\u30fc\u30b1\u30f3\u30b9\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u4f5c\u88fd
\n\u30fb\u30b7\u30fc\u30b1\u30f3\u30b7\u30f3\u30b0\u89e3\u6790\uff08PacBio Revio\uff09
\n\u30fb\u30c7\u30fc\u30bf\u89e3\u6790
\n\u30ea\u30fc\u30c9\u306e\u632f\u308a\u5206\u3051\u304a\u3088\u3073Index\u914d\u5217\u306e\u9664\u53bb\u3092\u884c\u3063\u305f\u5f8c\u3001\u5b8c\u5168\u9577\u30ea\u30fc\u30c9\u306e\u30ce\u30a4\u30ba\u3092\u9664\u53bb\u3057\u307e\u3059\u3002Iso-Seq3\u3092\u7528\u3044\u3066\u3001\u30d5\u30a3\u30eb\u30bf\u30ea\u30f3\u30b0\u3057\u305f\u5b8c\u5168\u9577\u30ea\u30fc\u30c9\u3092\u30af\u30e9\u30b9\u30bf\u30ea\u30f3\u30b0\u3057\u307e\u3059\u3002
\n\u305d\u306e\u5f8c\u3001\u53c2\u7167\u914d\u5217\u304c\u3042\u308b\u5834\u5408\u306f\u3001pbmm2\u3092\u7528\u3044\u3066\u30af\u30e9\u30b9\u30bf\u30ea\u30f3\u30b0\u3057\u305fhq.fasta\u3092\u53c2\u7167\u914d\u5217\u306b\u30de\u30c3\u30d4\u30f3\u30b0\u3092\u884c\u3044\u307e\u3059\u3002Iso-Seq3\u3092\u7528\u3044\u3066\u3001\u56fa\u6709\u306e\u30a2\u30a4\u30bd\u30d5\u30a9\u30fc\u30e0\u306b\u5206\u985e\u3057\u307e\u3059\u3002\u53c2\u7167gtf\u3082\u3042\u308b\u5834\u5408\u306f\u3001SQANTI3\u3092\u7528\u3044\u3066\u3001\u7d71\u5408\u3057\u305f\u30a2\u30a4\u30bd\u30d5\u30a9\u30fc\u30e0\u3092\u30a2\u30ce\u30c6\u30fc\u30b7\u30e7\u30f3\u3057\u307e\u3059\u3002<\/td>\n<\/tr>\n
\u7d0d\u54c1\u7269<\/th>\n\u4ee5\u4e0b\u306e\u30c7\u30fc\u30bf\u3092DVD\u3084USB\u7b49\u306e\u8a18\u9332\u5a92\u4f53\u306b\u4fdd\u5b58\u3057\u3066\u7d0d\u54c1\u3057\u307e\u3059\u3002<\/td>\n<\/tr>\n
\u2460\u30b7\u30fc\u30b1\u30f3\u30b9\u751f\u30c7\u30fc\u30bf\uff08fastq\uff06bam\u5f62\u5f0f\uff09
\n\u2461\u30af\u30e9\u30b9\u30bf\u30ea\u30f3\u30b0\u306e\u7d50\u679c (\u30a8\u30af\u30bb\u30eb\u30d5\u30a1\u30a4\u30eb\u3068fasta\u5f62\u5f0f)
\n\u2462DDBJ\u306e\u30c7\u30fc\u30bf\u30d9\u30fc\u30b9\u767b\u9332\u306b\u5fc5\u8981\u306a\u30c7\u30fc\u30bf<\/td>\n		\u2192\u53c2\u7167\u914d\u5217\u304c\u306a\u3051\u308c\u3070\u7d0d\u54c1\u7269\u306f\u2460\uff5e\u2462\u306e3\u70b9\u306e\u307f\u3067\u3059
\n\u7d0d\u54c1\u30c7\u30fc\u30bf\u306e\u30b5\u30f3\u30d7\u30eb<\/a><\/td>\n<\/tr>\n
\u2463\u56fa\u6709\u306e\u30a2\u30a4\u30bd\u30d5\u30a9\u30fc\u30e0\u306e\u5206\u985e\u30c7\u30fc\u30bf(gff\u5f62\u5f0f\u306a\u3069)
\n\u2464Mapping\u306e\u7d50\u679c(bam\u5f62\u5f0f)
\n\u2465\u53c2\u7167\u914d\u5217\uff08fasta&fasta.fai\u5f62\u5f0f\uff09<\/td>\n		\u2192\u53c2\u7167\u914d\u5217\u304c\u3042\u308b\u304c\u3001\u53c2\u7167gtf\u304c\u306a\u3051\u308c\u3070\u2460\uff5e\u2465\u3092\u7d0d\u54c1\u3057\u307e\u3059\u3002\u7d0d\u54c1\u30c7\u30fc\u30bf\u306e\u30b5\u30f3\u30d7\u30eb\uff08\u2460\uff5e\u2465\uff09<\/a><\/td>\n<\/tr>\n
\u2466Annotation\u7d50\u679c\uff08\u30a8\u30af\u30bb\u30eb\u30d5\u30a1\u30a4\u30eb\uff09
\n\u2467\u53c2\u7167\u907a\u4f1d\u5b50\u30a2\u30ce\u30c6\u30fc\u30b7\u30e7\u30f3\uff08gtf\u5f62\u5f0f\uff09<\/td>\n		\u2192\u53c2\u7167\u914d\u5217\u3068gtf\u3068\u3082\u3042\u308c\u3070\u3001\u2460\uff5e\u2467\u3092\u7d0d\u54c1\u3057\u307e\u3059\u3002\u7d0d\u54c1\u30c7\u30fc\u30bf\u306e\u30b5\u30f3\u30d7\u30eb\uff08\u2460\uff5e\u2467\uff09<\/a><\/td>\n<\/tr>\n
\u3054\u63d0\u4f9b
\n\u30b5\u30f3\u30d7\u30eb<\/th>\n		\u30c8\u30fc\u30bf\u30ebRNA 1 \u03bcg\u4ee5\u4e0a\/1\u30bb\u30eb\uff08\uff1e50ng\/ul\u3001\uff1e20ul\uff09
\n\u203bRNase-free water\u306b\u6eb6\u89e3\u3057\u305f\u72b6\u614b\u3067\u51b7\u51cd\u4fbf\u3092\u4f7f\u7528\u3057\u3066\u9001\u4ed8\u304f\u3060\u3055\u3044\u3002<\/td>\n<\/tr>\n
\u3054\u4f9d\u983c\u306e\u6d41\u308c<\/th>\n\u307e\u305a\u306f\u304a\u554f\u5408\u305b\u30d5\u30a9\u30fc\u30e0<\/a>\u304b\u3089\u304a\u898b\u7a4d\u308a\u3092\u3054\u4f9d\u983c\u304f\u3060\u3055\u3044\u3002<\/td>\n<\/tr>\n<\/tbody>\n<\/table><\/div>\n

\u203b1\u3000\u30b5\u30f3\u30d7\u30eb\u306b\u4f9d\u5b58\u3059\u308b\u305f\u3081\u3001\u30c7\u30fc\u30bf\u91cf\u306e\u4fdd\u8a3c\u306f\u3067\u304d\u307e\u305b\u3093\u3002\u307e\u305f\u30011\u30b5\u30f3\u30d7\u30eb\u5206\u306e\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u8abf\u88fd\u8cbb\u7528\u306f\u4e0a\u8a18\u306b\u542b\u307e\u308c\u3066\u3044\u307e\u3059\u3002\u8907\u6570\u30b5\u30f3\u30d7\u30eb\u3054\u4f9d\u983c\u306e\u5834\u5408\u306f\u30012\u30b5\u30f3\u30d7\u30eb\u76ee\u4ee5\u964d\u304b\u3089\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u8abf\u88fd\u8cbb\u7528100,000\u5186\/\u30b5\u30f3\u30d7\u30eb\u304c\u5225\u9014\u5fc5\u8981\u3067\u3059\u3002<\/p>\n

2.\u30aa\u30d7\u30b7\u30e7\u30f3<\/h3>\n
\n\n\n\n
\u9805\u76ee<\/th>\n\u5099\u8003<\/th>\n\u4fa1\u683c\uff08\u7a0e\u5225\uff09<\/th>\n\u8ffd\u52a0
\n\u7d0d\u671f<\/th>\n<\/tr>\n
RNA\u62bd\u51fa<\/td>\nQIAGEN\u793e\u306eRNeasy\u7b49\u3092\u4f7f\u7528\u3057\u3066\u3001\u30c8\u30fc\u30bf\u30ebRNA\u3092\u62bd\u51fa\u3057\u307e\u3059\u3002
\n\uff1c\u3054\u63d0\u4f9b\u30b5\u30f3\u30d7\u30eb\u91cf\u306e\u76ee\u5b89\uff1e
\n\u9175\u6bcd\uff1a\u7d30\u80de\u6570 5\u00d710^7\u7a0b\u5ea6\u3001\u6e7f\u91cd\u91cf 25 mg\u7a0b\u5ea6
\n\u690d\u7269\uff08\u8449\uff09\uff1a\u6e7f\u91cd\u91cf 50 mg\u7a0b\u5ea6\u3001\u76f4\u5f841.5cm
\n\u52d5\u7269\u7d30\u80de\uff1a\u7d30\u80de\u6570 1\u00d710^7\u7a0b\u5ea6\u3001\u6e7f\u91cd\u91cf 20 mg\u7a0b\u5ea6
\n\u52d5\u7269\u7d44\u7e54\uff1a\u6e7f\u91cd\u91cf 20 mg\u7a0b\u5ea6<\/td>\n		20,000\u5186\/\u30b5\u30f3\u30d7\u30eb<\/td>\n5\u55b6\u696d\u65e5<\/td>\n<\/tr>\n<\/tbody>\n<\/table><\/div>\n

2025\u5e746\u6708\u3000\u30c7\u30fc\u30bf\u89e3\u6790\u306e\u30aa\u30d7\u30b7\u30e7\u30f3\u306f\u7d42\u4e86\u3057\u307e\u3057\u305f\u3002<\/p>\n

3.\u3088\u304f\u3042\u308b\u8cea\u554f<\/h3>\n

FAQs\u30da\u30fc\u30b8<\/a>\u3092\u3054\u89a7\u304f\u3060\u3055\u3044\u3002<\/p>\n

4.\u3061\u3089\u3057PDF<\/h3>\n

\"pac\u30b2\u30ce\u30e0\u89e3\u6790\"Iso-seq\u89e3\u6790\uff08\u30ed\u30f3\u30b0\u30ea\u30fc\u30c9RNA-seq\u89e3\u6790\u30fbPacBio\u793e\uff09<\/a><\/p>\n

5.\u304a\u554f\u5408\u305b\u30fb\u30b5\u30f3\u30d7\u30eb\u9001\u4ed8\u5148<\/h3>\n

\u304a\u898b\u7a4d\u308a\u30fb\u304a\u554f\u5408\u305b<\/h4>\n

\u304a\u554f\u5408\u305b\u30d5\u30a9\u30fc\u30e0<\/a>\u3001\u30e1\u30fc\u30eb\uff08dna@gikenbio.com\uff09\u3001\u307e\u305f\u306f\u3000\u304a\u96fb\u8a71\uff08042-780-8333\uff09\u3067\u304a\u6c17\u8efd\u306b\u3054\u76f8\u8ac7\u304f\u3060\u3055\u3044\u3002<\/p>\n

\u30b5\u30f3\u30d7\u30eb\u9001\u4ed8\u5148<\/h4>\n

\u682a\u5f0f\u4f1a\u793e\u751f\u7269\u6280\u7814
\n\u3012252-0154\u3000\u795e\u5948\u5ddd\u770c\u76f8\u6a21\u539f\u5e02\u7dd1\u533a\u9577\u7af9657\u3000\/\u3000TEL\u3000042-780-8333
\n\u203b\u5e73\u65e5\u7740\u3067\u304a\u9001\u308a\u304f\u3060\u3055\u3044\u3002\u767a\u9001\u306f\u5143\u6255\u3044\u3067\u304a\u9858\u3044\u3057\u3066\u3044\u307e\u3059\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"1.Iso-seq\u89e3\u6790\uff08\u30ed\u30f3\u30b0\u30ea\u30fc\u30c9RNA-seq\u89e3\u6790\u30fbPacBio\uff09\u30b5\u30fc\u30d3\u30b9\u6982\u8981 \u5bfe\u8c61\u751f\u7269 \u771f\u6838\u751f\u7269\u306e\u307f\u3002 cDNA\u5408\u6210\u306boligo dT\u3092\u7528\u3044\u3066\u3044\u308b\u306e\u3067\u3001polyA\u3092\u6301\u305f\u306a\u3044\u751f\u7269\u306fLib\u8abf\u88fd\u3067\u304d\u305a\u89e3\u6790\u4e0d\u53ef\u3068\u306a\u308a\u307e\u3059\u3002 […]","protected":false},"author":1,"featured_media":0,"parent":10,"menu_order":8,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-5748","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/gikenbio.com\/2025test\/wp-json\/wp\/v2\/pages\/5748","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/gikenbio.com\/2025test\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/gikenbio.com\/2025test\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/gikenbio.com\/2025test\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/gikenbio.com\/2025test\/wp-json\/wp\/v2\/comments?post=5748"}],"version-history":[{"count":32,"href":"https:\/\/gikenbio.com\/2025test\/wp-json\/wp\/v2\/pages\/5748\/revisions"}],"predecessor-version":[{"id":6905,"href":"https:\/\/gikenbio.com\/2025test\/wp-json\/wp\/v2\/pages\/5748\/revisions\/6905"}],"up":[{"embeddable":true,"href":"https:\/\/gikenbio.com\/2025test\/wp-json\/wp\/v2\/pages\/10"}],"wp:attachment":[{"href":"https:\/\/gikenbio.com\/2025test\/wp-json\/wp\/v2\/media?parent=5748"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}