{"id":972,"date":"2016-07-28T12:34:14","date_gmt":"2016-07-28T03:34:14","guid":{"rendered":"https:\/\/gikenbio.com\/2025test\/?page_id=972"},"modified":"2025-04-25T17:21:18","modified_gmt":"2025-04-25T08:21:18","slug":"techinfo","status":"publish","type":"page","link":"https:\/\/gikenbio.com\/2025test\/dnaanalysis\/ngs\/amplicon\/techinfo\/","title":{"rendered":"\u30b5\u30f3\u30d7\u30eb\u8abf\u88fd\u306b\u3064\u3044\u3066\/\u30a2\u30f3\u30d7\u30ea\u30b3\u30f3\u30b7\u30fc\u30b1\u30f3\u30b9\u89e3\u6790(illumina)\u7528"},"content":{"rendered":"<p>\u5f0a\u793e\u3067\u306f\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u8abf\u88fd\u306b2-step tailed PCR\u6cd5\u3092\u7528\u3044\u3066\u304a\u308a\u307e\u3059\u3002<br \/>\n1st PCR\u306f\u76ee\u7684\u9818\u57df\u306e\u5897\u5e45\u3068\u30a2\u30c0\u30d7\u30bf\u30fc\u914d\u5217\uff082nd PCR\u7528\u306e\u30d7\u30e9\u30a4\u30de\u30fc\u3068\u306e\u76f8\u540c\u90e8\u5206\uff09\u306e\u4ed8\u4e0e\u30012nd PCR\u306f\u30b7\u30fc\u30b1\u30f3\u30b9\u30a2\u30c0\u30d7\u30bf\u30fc\u3068\u30b5\u30f3\u30d7\u30eb\u8b58\u5225\u7528\u306eindex\u306e\u4ed8\u4e0e\u3092\u884c\u3044\u307e\u3059\u3002<br \/>\n2-step tailed PCR\u6cd5\u306b\u3064\u3044\u3066\u306f\u3001<a href=\"https:\/\/jp.illumina.com\/content\/dam\/illumina-marketing\/apac\/japan\/documents\/pdf\/2014_techsupport_session5.pdf\" target=\"_blank\" rel=\"noopener\">\u30a4\u30eb\u30df\u30ca\u793e\u63d0\u4f9b\u306e\u8cc7\u6599<\/a>\u3092\u3054\u53c2\u7167\u304f\u3060\u3055\u3044\u3002<\/p>\n<p>\u5f0a\u793e\u3067\u306e\u30a2\u30f3\u30d7\u30ea\u30b3\u30f3\u30b7\u30fc\u30b1\u30f3\u30b9\u89e3\u6790\u3092\u3054\u691c\u8a0e\u3057\u3066\u304a\u3089\u308c\u3001\u304a\u5ba2\u69d8\u3067\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u8abf\u88fd\u3092\u884c\u308f\u308c\u308b\u5834\u5408\u306f\u3001\u5f0a\u793e\u3088\u308a\u30d7\u30e9\u30a4\u30de\u30fc\u3092\u9001\u4ed8\u3044\u305f\u3057\u307e\u3059\u3002<br \/>\n1st PCR\u7528\u30d7\u30e9\u30a4\u30de\u30fc\u306f1,500\u5186\uff08\u7a0e\u5225\u30fb\u9001\u6599\u8fbc\uff09\u3001<br \/>\n1st PCR\u7528\uff062nd PCR\u7528\u30d7\u30e9\u30a4\u30de\u30fc\u306f10,000\u5186\/96\u30b5\u30f3\u30d7\u30eb\uff08\u7a0e\u5225\u30fb\u9001\u6599\u8fbc\uff09\u3067\u3059\u3002<\/p>\n<p>\u30a2\u30f3\u30d7\u30ea\u30b3\u30f3\u30b7\u30fc\u30b1\u30f3\u30b9\u89e3\u6790\u306e\u300c5.\u5bfe\u8c61\u907a\u4f1d\u5b50\u9818\u57df\u300d\u306e\u30d7\u30e9\u30a4\u30de\u30fc\u30bb\u30c3\u30c8\u306f\u30b9\u30c8\u30c3\u30af\u304c\u3042\u308a\u307e\u3059\u306e\u3067\u3001\u3059\u3050\u306b\u3054\u63d0\u4f9b\u53ef\u80fd\u3067\u3059\u3002<br \/>\n\u307e\u305f\u3001\u30ea\u30b9\u30c8\u306b\u306a\u3044\u30d7\u30e9\u30a4\u30de\u30fc\u30bb\u30c3\u30c8\u3067\u306e\u89e3\u6790\u3082\u53ef\u80fd\u3067\u3059\u3002\u907a\u4f1d\u5b50\u7279\u7570\u7684\u90e8\u5206\u306e\u30d7\u30e9\u30a4\u30de\u30fc\u914d\u5217\u3092\u6559\u3048\u3066\u3044\u305f\u3060\u3051\u308c\u3070\u30011st PCR\u7528\u30d7\u30e9\u30a4\u30de\u30fc\u3092\u5408\u6210\u3057\u3066\u304a\u9001\u308a\u3057\u307e\u3059\u3002\u5f0a\u793e\u306b\u5728\u5eab\u304c\u306a\u3044\uff08\u904e\u53bb\u5408\u6210\u5c65\u6b74\u306e\u306a\u3044\uff09\u30d7\u30e9\u30a4\u30de\u30fc\u306b\u3064\u3044\u3066\u306f5,000\u5186\/\u30bb\u30c3\u30c8\uff08F\/R\u54041\u672c\u30fb\u7a0e\u5225\uff09\u304c\u304b\u304b\u308a\u307e\u3059\u3002\u307e\u305f\u3001\u9001\u4ed8\u4ee3\u3068\u3057\u3066\u4e0a\u8a18\u306e\u901a\u308a1,500\u5186\uff08\u7a0e\u5225\uff09\u304c\u304b\u304b\u308a\u307e\u3059\u3002<\/p>\n<h3 class=\"style3a\">\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u8abf\u88fd\u65b9\u6cd5\u306f\u4ee5\u4e0b\u3092\u53c2\u8003\u306b\u3057\u3066\u304f\u3060\u3055\u3044\u3002<\/h3>\n<h4 class=\"style4b\">1st PCR<\/h4>\n<p>1st PCR\u7528\u306e\u30d7\u30e9\u30a4\u30de\u30fc\u914d\u5217\u3068\u53cd\u5fdc\u6761\u4ef6\u4f8b<\/p>\n<ul>\n<li><a href=\"https:\/\/gikenbio.com\/2025test\/dnaanalysis\/ngs\/amplicon\/techinfo\/v4\/\" target=\"_blank\" rel=\"noopener\">16S rRNA V4\uff1a515F-806R<\/a><\/li>\n<li><a href=\"https:\/\/gikenbio.com\/2025test\/dnaanalysis\/ngs\/amplicon\/techinfo\/16s-rrna-v4-ver2\/\" target=\"_blank\" rel=\"noopener\">16S rRNA V4\uff08ver2\uff09\uff1a515F-806RB<\/a><\/li>\n<li><a href=\"https:\/\/gikenbio.com\/2025test\/dnaanalysis\/ngs\/amplicon\/techinfo\/v3v4\/\" target=\"_blank\" rel=\"noopener\">16S rRNA V3\/V4\uff1a341f-805r<\/a><\/li>\n<li><a href=\"https:\/\/gikenbio.com\/2025test\/dnaanalysis\/ngs\/amplicon\/techinfo\/v1v2\/\" target=\"_blank\" rel=\"noopener\">16S rRNA V1\/V2\uff1a27Fmod-338R<\/a><\/li>\n<li><a href=\"https:\/\/gikenbio.com\/2025test\/dnaanalysis\/ngs\/amplicon\/techinfo\/its1\/\" target=\"_blank\" rel=\"noopener\">ITS1\u9818\u57df\uff1aITS1F_KYO1 \u2013 ITS2_KYO2<\/a><\/li>\n<li><a href=\"https:\/\/gikenbio.com\/2025test\/dnaanalysis\/ngs\/amplicon\/techinfo\/its2-g\/\" target=\"_blank\" rel=\"noopener\">ITS2\u9818\u57df\uff1agITS7 \u2013 ITS4<\/a><\/li>\n<li><a href=\"https:\/\/gikenbio.com\/2025test\/dnaanalysis\/ngs\/amplicon\/techinfo\/its2-f\/\" target=\"_blank\" rel=\"noopener\">ITS2\u9818\u57df\uff1afITS7 \u2013 ITS4<\/a><\/li>\n<li><a href=\"https:\/\/gikenbio.com\/2025test\/dnaanalysis\/ngs\/amplicon\/techinfo\/18s\/\" target=\"_blank\" rel=\"noopener\">18S rRNA\uff1a1422f-1642r<\/a><\/li>\n<li><a href=\"https:\/\/gikenbio.com\/2025test\/dnaanalysis\/ngs\/amplicon\/techinfo\/18s-rrna-ver2\/\" target=\"_blank\" rel=\"noopener\">18S rRNA ver2\uff1aV8f-1510r <\/a><\/li>\n<li><a href=\"https:\/\/gikenbio.com\/2025test\/dnaanalysis\/ngs\/amplicon\/techinfo\/coi\/\" target=\"_blank\" rel=\"noopener\">COI\uff1aIntF-HCOmR<\/a><\/li>\n<li><a href=\"https:\/\/gikenbio.com\/2025test\/dnaanalysis\/ngs\/amplicon\/techinfo\/coi-block\/\" target=\"_blank\" rel=\"noopener\">COI\uff1aIntF-HCOmR\uff0b\u30d6\u30ed\u30c3\u30ad\u30f3\u30b0<\/a><\/li>\n<li><a href=\"https:\/\/gikenbio.com\/2025test\/dnaanalysis\/ngs\/amplicon\/techinfo\/mifish\/\" target=\"_blank\" rel=\"noopener\">12S rRNA (cMiFish)<\/a><\/li>\n<li><a href=\"https:\/\/gikenbio.com\/2025test\/dnaanalysis\/ngs\/amplicon\/techinfo\/mimammal\/\" target=\"_blank\" rel=\"noopener\">12S rRNA (MiMammal)<\/a><\/li>\n<\/ul>\n<h5 class=\"style5a\">1st PCR\u306b\u3064\u3044\u3066\u3088\u304f\u3042\u308b\u8cea\u554f<\/h5>\n<p><strong>Q1. \u30c6\u30f3\u30d7\u30ec\u30fc\u30c8DNA\u306e\u91cf\u306f\uff1f<\/strong><br \/>\nA1. \u7d4c\u9a13\u4e0a1\uff5e5ng\u7a0b\u5ea6\u306eDNA\u91cf\u3092\u63a8\u5968\u3057\u3066\u304a\u308a\u307e\u3059\u3002<br \/>\n\u307e\u305f\u3001DNA\u62bd\u51fa\u306b\u3064\u3044\u3066\u306f\u5e02\u8ca9\u306eDNA\u62bd\u51fa\u30ad\u30c3\u30c8\u3092\u3054\u5229\u7528\u3057\u3066\u3044\u305f\u3060\u3051\u308c\u3070\u554f\u984c\u3054\u3056\u3044\u307e\u305b\u3093\u3002<br \/>\n<a href=\"https:\/\/gikenbio.com\/2025test\/look-up\/extractionkit\/\" target=\"_blank\" rel=\"noopener noreferrer\">\u62bd\u51fa\u30ad\u30c3\u30c8\u3068\u306e\u6bd4\u8f03\u691c\u8a3c\u30c7\u30fc\u30bf\u306f\u3053\u3061\u3089<\/a><\/p>\n<p><strong>Q2. 1stPCR\u306e\u30b5\u30a4\u30af\u30eb\u6570\u306e\u76ee\u5b89\u306f\uff1f<\/strong><br \/>\nA2. 25-35\u30b5\u30a4\u30af\u30eb\u304c\u76ee\u5b89\u3067\u3059\u300235\u30b5\u30a4\u30af\u30eb\u3092\u8d85\u3048\u308b\u3068\u975e\u7279\u7570\u7684\u306a\u5897\u5e45\uff08\u30cd\u30ac\u30c6\u30a3\u30d6\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\u3067\u3082\u5897\u5e45\u3057\u3066\u3057\u307e\u3046\uff09\u304c\u8d77\u3053\u308b\u53ef\u80fd\u6027\u304c\u9ad8\u304f\u306a\u308a\u307e\u3059\u3002<\/p>\n<p><strong>Q3. \u7cbe\u88fd\u306f\u5fc5\u8981\uff1f<\/strong><br \/>\nA3. \u5f0a\u793e\u306b1st PCR\u7523\u7269\u3092\u9001\u308b\u5834\u5408\u306f\u30011st PCR\u53cd\u5fdc\u6eb6\u6db2\u306e\u307e\u307e\u304a\u9001\u308a\u3044\u305f\u3060\u3044\u3066\u69cb\u3044\u307e\u305b\u3093\u3002\u5f0a\u793e\u3067\u7cbe\u88fd\u3044\u305f\u3057\u307e\u3059\u3002<br \/>\n\u304a\u5ba2\u69d8\u81ea\u8eab\u30672nd PCR\u307e\u3067\u884c\u3046\u5834\u5408\u30011stPCR\u5f8c\u306e\u7cbe\u88fd\u306f\u5b9f\u65bd\u3057\u3066\u304f\u3060\u3055\u3044\u3002\u7cbe\u88fd\u306e\u76ee\u7684\u306f\u4f7f\u7528\u3057\u305f\u30d7\u30e9\u30a4\u30de\u30fc\u306e\u9664\u53bb\uff08\u304a\u304a\u3088\u305d 100bp \u4ee5\u4e0b\uff09\u3067\u3059\u306e\u3067\u3001\u30d3\u30fc\u30ba\u7cbe\u88fd\u30fb\u30ab\u30e9\u30e0\u7cbe\u88fd\u30fb\u30b2\u30eb\u5207\u308a\u51fa\u3057\u3044\u305a\u308c\u306e\u65b9\u6cd5\u3067\u3082\u554f\u984c\u3042\u308a\u307e\u305b\u3093\u3002<\/p>\n<p><strong>Q4. \u96fb\u6c17\u6cf3\u52d5\u306b\u3088\u308b\u5897\u5e45\u7523\u7269\u306e\u78ba\u8a8d\u306f\u5fc5\u8981\uff1f<\/strong><br \/>\nA4. \u5fc5\u9808\u3067\u306f\u3042\u308a\u307e\u305b\u3093\u304c\u3001\u96fb\u6c17\u6cf3\u52d5\u304c\u3067\u304d\u308b\u74b0\u5883\u3067\u3042\u308c\u3070\u3054\u78ba\u8a8d\u3044\u305f\u3060\u304f\u3053\u3068\u3092\u304a\u52e7\u3081\u3057\u307e\u3059\u3002<br \/>\n\u5f0a\u793e\u3067\u306f\u3001\u9001\u4ed8\u3044\u305f\u3060\u3044\u305f1st PCR\u7523\u7269\u3092\u7cbe\u88fd\u5f8c\u3001\u6fc3\u5ea6\u6e2c\u5b9a\u306e\u307f\u884c\u3044\u307e\u3059\u3002\u6fc3\u5ea6\u6e2c\u5b9a\u306e\u7d50\u679c\u304c\u5b9a\u91cf\u4e0b\u9650\u5024\u672a\u6e80\u3067\u30822nd PCR\u3078\u9032\u307f\u307e\u3059\u30022nd PCR\u3067\u76ee\u7684\u306e\u5897\u5e45\u7523\u7269\u304c\u78ba\u8a8d\u3055\u308c\u306a\u304b\u3063\u305f\u30b5\u30f3\u30d7\u30eb\u306f\u3001\u5bfe\u8c61DNA\u306e\u5897\u5e45\u306a\u3057\u306e\u7d50\u679c\u3092\u3082\u3063\u3066\u7d42\u4e86\u3068\u306a\u308a\u307e\u3059\uff08\u3064\u307e\u308a\u3001\u30b7\u30fc\u30b1\u30f3\u30b7\u30f3\u30b0\u89e3\u6790\u306f\u884c\u308f\u306a\u3044\uff09\u304c\u3001\u89e3\u6790\u8cbb\u7528\u306f\u6e80\u984d\u306e\u3054\u8acb\u6c42\u3068\u306a\u308a\u307e\u3059\u3002<\/p>\n<p><strong>Q5. \u96fb\u6c17\u6cf3\u52d5\u306e\u7d50\u679c\u3001\u975e\u7279\u7570\u5897\u5e45\u7523\u7269\u304c\u78ba\u8a8d\u3055\u308c\u305f\u5834\u5408\u306f\u3069\u3046\u3057\u305f\u3089\u3088\u3044\uff1f<\/strong><br \/>\nA5. \u76ee\u7684\u306e\u5897\u5e45\u7523\u7269\u306e\u307f\u3068\u306a\u308b\u3088\u3046\u3001\u7cbe\u88fd\u3059\u308b\u3053\u3068\u3092\u304a\u52e7\u3081\u3057\u307e\u3059\u3002<br \/>\n\u7cbe\u88fd\u3057\u306a\u3044\u5834\u5408\u3001\u30b7\u30fc\u30b1\u30f3\u30b7\u30f3\u30b0\u89e3\u6790\u3067\u975e\u7279\u7570\u5897\u5e45\u7523\u7269\u7531\u6765\u306e\u30c7\u30fc\u30bf\u3082\u53d6\u5f97\u3055\u308c\u308b\u305f\u3081\u3001\u76ee\u7684\u306e\u5897\u5e45\u7523\u7269\u306e\u30c7\u30fc\u30bf\u304c\u6e1b\u3063\u3066\u3057\u307e\u3046\u3053\u3068\u306f\u3042\u3089\u304b\u3058\u3081\u3054\u4e86\u627f\u304f\u3060\u3055\u3044\u3002<br \/>\n\u203b\u6ce8\u610f\uff1aITS\u9818\u57df\u306f\u7a2e\u306b\u3088\u308a\u9577\u3055\u304c\u7570\u306a\u308a\u307e\u3059\u3002\u30b2\u30eb\u5207\u308a\u51fa\u3057\u306b\u3088\u308b\u7cbe\u88fd\u306f\u30c7\u30fc\u30bf\u306e\u30d0\u30a4\u30a2\u30b9\u306e\u539f\u56e0\u3068\u306a\u308b\u5834\u5408\u304c\u3042\u308a\u307e\u3059\u306e\u3067\u3001ITS\u9818\u57df\u306e\u5897\u5e45\u7523\u7269\u306e\u7cbe\u88fd\u306f\u304a\u52e7\u3081\u3067\u304d\u307e\u305b\u3093\u3002<\/p>\n<p><strong>Q6. 1stPCR\u7528\u30d7\u30e9\u30a4\u30de\u30fc\u306e\u9001\u4ed8\u91cf\u306f\u3069\u308c\u304f\u3089\u3044\uff1f<\/strong><br \/>\nA6. 1\u691c\u4f53\u3042\u305f\u308a1 ul\u4f7f\u7528\u3059\u308b\u3068\u4eee\u5b9a\u3057\u3066\u30013\u56de\u4ee5\u4e0aPCR\u5b9f\u65bd\u53ef\u80fd\u306a\u91cf\u3092\u9001\u4ed8\u3057\u3066\u3044\u307e\u3059\u3002<br \/>\n\u4f8b\uff0924\u691c\u4f53\u3054\u4f9d\u983c\u306e\u5834\u5408\uff1a1 ul\/\u691c\u4f53\u00d724\u691c\u4f53\u00d73\uff1d72 ul\u3000\u2192FR\u5404100ul\u00d71\u672c\u9001\u4ed8\u3057\u307e\u3059\u3002<\/p>\n<h4 class=\"style4b\">2nd PCR<\/h4>\n<p>\u4ee5\u4e0b\u306f\u3001 PCR \u9175\u7d20\u306b KOD FX Neo [TOYOBO]\u3092\u4f7f\u7528\u3057\u305f\u4f8b\u3067\u3059\u3002<br \/>\n\u4ed6\u306e PCR \u9175\u7d20\u3092\u4f7f\u7528\u3059\u308b\u5834\u5408\u306f\u3001\u305d\u306ePCR\u9175\u7d20\u63a8\u5968\u306e\u53cd\u5fdc\u7d44\u6210\u30fb\u53cd\u5fdc\u6761\u4ef6\u3067\u8abf\u88fd\u3057\u3066\u304f\u3060\u3055\u3044\u3002<\/p>\n<div id=\"sosei\">\n<p>\u25cf\u53cd\u5fdc\u7d44\u6210<\/p>\n<dl>\n<dt>2\u00d7 PCR Buffer for KOD FX Neo<\/dt>\n<dd>10.0 ul<\/dd>\n<dt>dNTPs (each 2 mM)<\/dt>\n<dd>4.0 ul<\/dd>\n<dt>2nd primer mix (each 2.5uM)<\/dt>\n<dd>2.0 ul<\/dd>\n<dt>1st PCR Product<\/dt>\n<dd>2.0ul<\/dd>\n<dt>KOD FX Neo (1.0 U\/uL)[TOYOBO]<\/dt>\n<dd>0.4 ul<\/dd>\n<dt class=\"underline\">Nuclease-Free Water<\/dt>\n<dd class=\"underline\">1.6 ul<\/dd>\n<dt>Total<\/dt>\n<dd>20 ul<\/dd>\n<\/dl>\n<\/div>\n<div id=\"ondo\">\n<p>&nbsp;<\/p>\n<p>\u25cf\u53cd\u5fdc\u6e29\u5ea6<\/p>\n<dl>\n<dt>94\u00b0C<\/dt>\n<dd>2min<\/dd>\n<dt>98\u00b0C<\/dt>\n<dd>10sec\uff5c<\/dd>\n<dt>60\u00b0C<\/dt>\n<dd>30sec\uff5c8-12 cycles<\/dd>\n<dt>68\u00b0C<\/dt>\n<dd>30sec\uff5c<\/dd>\n<dt>68\u00b0C<\/dt>\n<dd>2min<\/dd>\n<\/dl>\n<\/div>\n<p>&nbsp;<\/p>\n<p>\u25cf2nd PCR\u7528\u30d7\u30e9\u30a4\u30de\u30fc\u914d\u5217\uff085\u2019\u21923\u2019\uff09\/\u30a2\u30f3\u30d7\u30ea\u30b3\u30f3\u30b7\u30fc\u30b1\u30f3\u30b9\u89e3\u6790\uff08illumina MiSeq\uff09\u7528<br \/>\n2nd Forward primer\uff1a<br \/>\nAATGATACGGCGACCACCGAGATCTACAC-index*-ACACTCTTTCCCTACACGACGC<br \/>\n2nd Reverse primer\uff1a<br \/>\nCAAGCAGAAGACGGCATACGAGAT-index*-GTGACTGGAGTTCAGACGTGTG<br \/>\n*\u30b5\u30f3\u30d7\u30eb\u8b58\u5225\u7528\u306e\u914d\u5217\u3067\u3059<\/p>\n<h5 class=\"style5a\">2nd PCR\u306b\u3064\u3044\u3066\u3088\u304f\u3042\u308b\u8cea\u554f<\/h5>\n<p><strong>Q1. \u7cbe\u88fd\u306f\u5fc5\u8981\uff1f<\/strong><br \/>\nA1. \u7cbe\u88fd\u306f\u5fc5\u305a\u5b9f\u65bd\u3057\u3066\u304f\u3060\u3055\u3044\u3002\u7cbe\u88fd\u306e\u76ee\u7684\u306f\u4f7f\u7528\u3057\u305f\u30d7\u30e9\u30a4\u30de\u30fc\u306e\u9664\u53bb\u3067\u3059\u3002<br \/>\n2ndPCR\u306b\u7528\u3044\u308b\u30d7\u30e9\u30a4\u30de\u30fc\u306fMiSeq\u3067\u306e\u89e3\u6790\uff08SBS\u6cd5\uff09\u306b\u5fc5\u8981\u306a\u914d\u5217\u3092\u542b\u3093\u3067\u304a\u308a\u307e\u3059\u3002MiSeq\u3067\u306e\u89e3\u6790\u6642\u306b2ndPCR\u306b\u7528\u3044\u308b\u30d7\u30e9\u30a4\u30de\u30fc\u304c\u542b\u307e\u308c\u3066\u3044\u308b\u3068\u30012ndPCR\u7528\u30d7\u30e9\u30a4\u30de\u30fc\u304c\u512a\u5148\u7684\u306b\u89e3\u6790\u3055\u308c\u3066\u3057\u307e\u3044\u3001\u30c7\u30fc\u30bf\u306e\u53d6\u5f97\u304c\u56f0\u96e3\u3068\u306a\u308b\u53ef\u80fd\u6027\u304c\u3054\u3056\u3044\u307e\u3059\u3002<\/p>\n<p>\u5f0a\u793e\u3067\u306f\u30d3\u30fc\u30ba\u7cbe\u88fd\u3092\u884c\u3063\u3066\u3044\u307e\u3059\u3002<br \/>\n\u7cbe\u88fd\u65b9\u6cd5\u4f8b\uff0920ul \u306e PCR \u7523\u7269\u306b\u5bfe\u3057\u3066\u3001Agencourt AMPure XP \u3092 20ul \u6dfb\u52a0\u3059\u308b\u300280\uff05\u30a8\u30bf\u30ce\u30fc\u30eb\u3067 wash \u3092 2 \u56de\u884c\u3044\u300110mM Tris-HCl (pH 8.0) 20ul \u3067\u6eb6\u51fa\u3059\u308b\u3002<\/p>\n<p>\u30ab\u30e9\u30e0\u7cbe\u88fd\u3067\u3082\u554f\u984c\u3042\u308a\u307e\u305b\u3093\u3002\u30b2\u30eb\u5207\u308a\u51fa\u3057\u306f\u6fc3\u5ea6\u4e0d\u8db3\u306b\u306a\u308b\u53ef\u80fd\u6027\u304c\u3042\u308b\u306e\u3067\u63a8\u5968\u3057\u307e\u305b\u3093\u3002<\/p>\n<p><strong>Q2. \u5fc5\u8981\u6fc3\u5ea6\u3068\u6db2\u91cf\u306f\uff1f<\/strong><br \/>\nA2. \u6fc3\u5ea610 ng\/ul\u4ee5\u4e0a\u3001\u6db2\u91cf20 ul\u4ee5\u4e0a\u3067\u3059\u3002\u6fc3\u5ea6\u304c\u6e80\u305f\u306a\u3044\u5834\u5408\u306f\u3054\u76f8\u8ac7\u304f\u3060\u3055\u3044\u3002<\/p>\n<p><strong>Q3. \u30b5\u30f3\u30d7\u30eb\u9001\u4ed8\u6642\u306e\u5bb9\u5668\u306f\uff1f<\/strong><br \/>\nA3. 8\u9023\u30c1\u30e5\u30fc\u30d6\u307e\u305f\u306f96\u7a74\u30d7\u30ec\u30fc\u30c8\u3067\u9001\u4ed8\u3057\u3066\u304f\u3060\u3055\u3044\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"\u5f0a\u793e\u3067\u306f\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u8abf\u88fd\u306b2-step tailed PCR\u6cd5\u3092\u7528\u3044\u3066\u304a\u308a\u307e\u3059\u3002 1st PCR\u306f\u76ee\u7684\u9818\u57df\u306e\u5897\u5e45\u3068\u30a2\u30c0\u30d7\u30bf\u30fc\u914d\u5217\uff082nd PCR\u7528\u306e\u30d7\u30e9\u30a4\u30de\u30fc\u3068\u306e\u76f8\u540c\u90e8\u5206\uff09\u306e\u4ed8\u4e0e\u30012nd PCR\u306f\u30b7\u30fc\u30b1\u30f3\u30b9\u30a2\u30c0\u30d7\u30bf\u30fc\u3068\u30b5 [&hellip;]","protected":false},"author":1,"featured_media":0,"parent":41,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-972","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/gikenbio.com\/2025test\/wp-json\/wp\/v2\/pages\/972","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/gikenbio.com\/2025test\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/gikenbio.com\/2025test\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/gikenbio.com\/2025test\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/gikenbio.com\/2025test\/wp-json\/wp\/v2\/comments?post=972"}],"version-history":[{"count":92,"href":"https:\/\/gikenbio.com\/2025test\/wp-json\/wp\/v2\/pages\/972\/revisions"}],"predecessor-version":[{"id":6719,"href":"https:\/\/gikenbio.com\/2025test\/wp-json\/wp\/v2\/pages\/972\/revisions\/6719"}],"up":[{"embeddable":true,"href":"https:\/\/gikenbio.com\/2025test\/wp-json\/wp\/v2\/pages\/41"}],"wp:attachment":[{"href":"https:\/\/gikenbio.com\/2025test\/wp-json\/wp\/v2\/media?parent=972"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}