\u5b9f\u9a13\u5ba4\u306e\u30b3\u30f3\u30bf\u30df\u30cd\u30fc\u30b7\u30e7\u30f3\u691c\u8a3c\u306e\u7d50\u679c\u304c\u51fa\u307e\u3057\u305f\u3002\u4eca\u56de\u306f\u304d\u3061\u3093\u3068\u304a\u4f1d\u3048\u3059\u308b\u305f\u3081\u6280\u8853\u7684\u306a\u5185\u5bb9\u306b\u306a\u3063\u3066\u3057\u307e\u3044\u307e\u3059\u3002<\/p>\n
\u5b9f\u9a13\u5185\u5bb9\u3067\u3059\u304c\u3001\u5b9f\u9a13\u5ba4\u306e\u5927\u6c17\u309224\u6642\u9593\u307b\u3069\u30d5\u30a3\u30eb\u30bf\u30fc\u306b\u30b5\u30f3\u30d7\u30ea\u30f3\u30b0\u3057\u307e\u3057\u305f\uff08\u76f4\u5f8413mm\u306e\u30d5\u30a3\u30eb\u30bf\u30fc2\u679a\uff09\u3002\u305d\u308c\u3092DNA\u3092\u6eb6\u89e3\u3055\u305b\u308bbuffer\uff08EB buffer\uff09\u306b\u6f2c\u3051\u8fbc\u307f\u3001\u51b7\u8535\u3067\u6570\u65e5\u4fdd\u7ba1\u3002\u305d\u306e\u6eb6\u6db2\u3092\u4f7f\u3063\u30662ndPCR\u3092\u884c\u3044\u307e\u3057\u305f\u3002<\/p>\n
2nd PCR\u306f1st PCR\u3067\u4ed8\u52a0\u3057\u305f\u30b7\u30fc\u30b1\u30f3\u30b7\u30f3\u30b0\u306b\u5fc5\u8981\u306a\u4eba\u5de5\u7684\u306a\u914d\u5217\u3092\u30bf\u30fc\u30b2\u30c8\u3063\u3068\u3057\u3066\u3044\u308b\u306e\u3067\u30011st PCR\u7523\u7269\u3042\u308b\u3044\u306f2nd PCR\u7523\u7269\u3067\u306a\u3051\u308c\u3070\u5897\u5e45\u306e\u92f3\u578b\u3068\u306a\u308a\u307e\u305b\u3093\u30022nd PCR\u306e\u7d50\u679c\u308f\u305a\u304b\u306b\u5897\u5e45\u304c\u78ba\u8a8d\u3055\u308c\u307e\u3057\u305f\u3002\u96fb\u6c17\u6cf3\u52d5\u306e\u7d50\u679c\u306f\u660e\u77ad\u306a\u30d4\u30fc\u30af\u304c\u78ba\u8a8d\u3055\u308c\u306a\u304b\u3063\u305f\u3082\u306e\u306e\uff08\u898b\u305f\u76ee\u306b\u306f\u5897\u5e45\u3057\u3066\u3044\u306a\u3044\uff09\u3001\u7121\u7406\u3084\u308a\u30b7\u30fc\u30b1\u30f3\u30b9\u3057\u3066\u30c7\u30fc\u30bf\u3092\u51fa\u3057\u307e\u3057\u305f\u3002<\/p>\n
\u7d50\u679c\u3068\u3057\u3066\u306f\u30ea\u30fc\u30c91\u304a\u3088\u3073\u30ea\u30fc\u30c92\u3067\u305d\u308c\u305e\u308c\u308f\u305a\u304b20\u30ea\u30fc\u30c9\u3061\u3087\u3063\u3068\u304c\u3001\u30d0\u30af\u30c6\u30ea\u30a2\u3001\u771f\u83cc\u3001\u9b5a\u985e\u3001\u690d\u7269\u306bBlast\u89e3\u6790\u306e\u7d50\u679c\u30d2\u30c3\u30c8\u3057\u307e\u3057\u305f\u3002\u30a4\u30eb\u30df\u30ca\u793e\u306e\u30b7\u30fc\u30b1\u30f3\u30b5\u30fc\u306e\u6280\u8853\u7684\u554f\u984c\u70b9\u3067\u3042\u308bindex\u306e\u632f\u308a\u5206\u3051\u30df\u30b9\u3088\u308a\u3082\u4f4e\u3044\u30ec\u30d9\u30eb\u3067\u3059\u3002\u306a\u306e\u3067\u3001\u3053\u306e\u7d50\u679c\u304c\u5b9f\u9a13\u5ba4\u306e\u7a7a\u6c17\u4e2d\u7531\u6765\u306e\u30c7\u30fc\u30bf\u3067\u3042\u308b\u306e\u304b\u3001\u3042\u308b\u3044\u306findex\u632f\u308a\u5206\u3051\u30df\u30b9\u3067\u3042\u308b\u306e\u304b\u3092\u65ad\u5b9a\u3059\u308b\u3053\u3068\u3082\u3067\u304d\u306a\u3044\u3067\u3059\u304c\u3001\u3053\u306e\u7d50\u679c\u304b\u3089\u306f\u5b9f\u9a13\u5ba4\u5185\u3067\u30d0\u30a4\u30aa\u30a8\u30a2\u30ed\u30be\u30eb\u5316\u3057\u305fPCR\u7523\u7269\u304c\u30b3\u30f3\u30bf\u30df\u30cd\u30fc\u30b7\u30e7\u30f3\u306e\u539f\u56e0\u3067\u3042\u308b\u53ef\u80fd\u6027\u306f\u975e\u5e38\u306b\u4f4e\u3044\u3068\u3044\u3046\u3053\u3068\u304c\u8a00\u3048\u307e\u3059\u3002\u307e\u30601\u56de\u3057\u304b\u3084\u3063\u3066\u3044\u306a\u3044\u306e\u3067\u3001\u3053\u308c\u304b\u3089\u3082\u5b9a\u671f\u7684\u306b\u30b5\u30f3\u30d7\u30ea\u30f3\u30b0\u3057\u3066\u30c7\u30fc\u30bf\u53d6\u5f97\u3057\u3066\u3044\u3053\u3046\u3068\u601d\u3063\u3066\u304a\u308a\u307e\u3059\u3002<\/p>\n
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