{"id":3200,"date":"2019-07-07T16:38:22","date_gmt":"2019-07-07T07:38:22","guid":{"rendered":"https:\/\/gikenbio.com\/2025test\/?p=3200"},"modified":"2020-06-28T11:44:37","modified_gmt":"2020-06-28T02:44:37","slug":"mgiseq%e3%81%a8%e3%83%8f%e3%82%a4%e3%83%96%e3%83%aa%e3%83%83%e3%83%89%e3%82%a2%e3%82%bb%e3%83%b3%e3%83%96%e3%83%ab","status":"publish","type":"post","link":"https:\/\/gikenbio.com\/2025test\/2019\/07\/07\/mgiseq%e3%81%a8%e3%83%8f%e3%82%a4%e3%83%96%e3%83%aa%e3%83%83%e3%83%89%e3%82%a2%e3%82%bb%e3%83%b3%e3%83%96%e3%83%ab\/","title":{"rendered":"MGISEQ\u3068\u30cf\u30a4\u30d6\u30ea\u30c3\u30c9\u30a2\u30bb\u30f3\u30d6\u30eb"},"content":{"rendered":"

\u6765\u9031\u65b0\u3057\u3044\u30b7\u30fc\u30b1\u30f3\u30b5\u30fc\u3092\u5c0e\u5165\u3057\u307e\u3059\u3002\u30a4\u30eb\u30df\u30ca\u793e\u306e\u30b7\u30fc\u30b1\u30f3\u30b5\u30fc\u3067\u306f\u306a\u304f\u4e2d\u56fd\u88fd\u306eMGISEQ2000\u3068\u3044\u3046\u6a5f\u7a2e\u3067\u3059\u3002<\/p>\n

\u306a\u3093\u306e\u3053\u3063\u3061\u3083\uff1f\u3063\u3066\u3044\u3046\u4eba\u304c\u307b\u3068\u3093\u3069\u3060\u3068\u601d\u3044\u307e\u3059\u304c\u3001\u5b9f\u306f\u6a2a\u6d5c\u306e\u7406\u5316\u5b66\u7814\u7a76\u6240\u306b\u306f3\u53f0\u5c0e\u5165\u3055\u308c\u3066\u304a\u308a\u3001\u4e00\u5e74\u4ee5\u4e0a\u524d\u304b\u3089\u7a3c\u50cd\u3057\u3066\u304a\u308a\u307e\u3059\u3002\u81ea\u5206\u3082\u898b\u5b66\u306b\u884c\u304b\u305b\u3066\u3044\u305f\u3060\u3044\u3066\u5b9f\u969b\u306b\u4f7f\u3063\u3066\u3044\u308b\u4eba\u306e\u304a\u8a71\u3057\u3092\u76f4\u63a5\u805e\u304f\u3053\u3068\u304c\u3067\u304d\u307e\u3057\u305f\u3002\u307e\u305f\u3001\u60c5\u5831\u53ce\u96c6\u3060\u3051\u3067\u306a\u304f\u3001\u9b5a\u985e\u306e\u30b2\u30ce\u30e0DNA\u3092\u30b5\u30f3\u30d7\u30eb\u3068\u3057\u3066\u30a4\u30eb\u30df\u30ca\u793e\u306eHiSeq\u3068MGISEQ2000\u306e\u6027\u80fd\u6bd4\u8f03\u3092\u884c\u3044\u307e\u3057\u305f\u3002\u5177\u4f53\u7684\u306b\u306f\u30d2\u30e9\u30e1\u3068\u30de\u30c0\u30a4\u306a\u306e\u3067\u3059\u304c\u3001\u4e0b\u8a18\u306f\u30d2\u30e9\u30e1\u306e\u30a2\u30bb\u30f3\u30d6\u30eb\u306e\u7d50\u679c\u3067\u3059\u3002\u307b\u307c\u540c\u3058\u3067\u3059\uff08\u5c11\u3057MGISEQ2000\u306e\u7528\u304c\u307e\u3068\u307e\u3063\u3066\u3044\u308b\uff09\u3002Q30\u7b49\u306e\u751f\u30c7\u30fc\u30bf\u306e\u54c1\u8cea\u306b\u95a2\u3059\u308b\u6bd4\u8f03\u3082\u884c\u3063\u3066\u304a\u308a\u307e\u3059\u304c\u3001\u305d\u308c\u3082\u307b\u307c\u540c\u3058\u3067\u3057\u305f\u3002<\/p>\n

\n\n\n\n\n\n\n\n\n\n
\u30b7\u30fc\u30b1\u30f3\u30b5\u30fc<\/td>\nMGISEQ2000<\/td>\nIllumina<\/td>\n<\/tr>\n
\u30ea\u30fc\u30c9\u9577<\/td>\n150bp<\/td>\n150bp<\/td>\n<\/tr>\n
\u30ea\u30fc\u30c9\u6570<\/td>\n211,723,905<\/td>\n292,651,766<\/td>\n<\/tr>\n
\u30a2\u30bb\u30f3\u30d6\u30e9\u30fc<\/td>\nMaSuRCA<\/td>\nMaSuRCA<\/td>\n<\/tr>\n
scaffolds\u6570<\/td>\n62,791<\/td>\n66,099<\/td>\n<\/tr>\n
\u6700\u9577scaffolds(bp)<\/td>\n553,059<\/td>\n230,252<\/td>\n<\/tr>\n
N50(bp)<\/td>\n28,809<\/td>\n24,879<\/td>\n<\/tr>\n
\u7dcf\u5869\u57fa\u6570(Mbp)<\/td>\n565,690,090<\/td>\n566,950,624<\/td>\n<\/tr>\n<\/tbody>\n<\/table><\/div>\n

\u3053\u30531\u304b\u6708\u307b\u3069\u5317\u6d77\u9053\u304b\u3089\u4e5d\u5dde\u307e\u3067\u5168\u56fd\u306e\u304a\u5ba2\u69d8\u3092\u8a2a\u554f\u3055\u305b\u3066\u3044\u305f\u3060\u3044\u305f\u306e\u3067\u3059\u304c\u3001\u3059\u3067\u306bMGISEQ\u306e\u3053\u3068\u3092\u77e5\u3063\u3066\u3044\u308b\u65b9\u3082\u7d50\u69cb\u3044\u307e\u3057\u305f\u3002\u4e2d\u306b\u306f\u3059\u3067\u306b\u53d7\u8a17\u89e3\u6790\u30b5\u30fc\u30d3\u30b9\u3067\u4f7f\u3063\u305f\u3053\u3068\u304c\u3042\u308b\u3068\u3044\u3046\u65b9\u3082\u3044\u307e\u3057\u305f\u3002<\/p>\n

\u305b\u3063\u304b\u304f\u30c7\u30fc\u30bf\u3092\u53d6\u5f97\u3057\u305f\u306e\u3067\u3001\u3055\u3089\u306b\u4fee\u884c\u76ee\u7684\u3067nanopore\u306e\u30c7\u30fc\u30bf\u3082\u53d6\u5f97\u3057\u3066\u30cf\u30a4\u30d6\u30ea\u30c3\u30c9\u30a2\u30bb\u30f3\u30d6\u30eb\u3092\u884c\u3044\u307e\u3057\u305f\u3002<\/p>\n

\n\n\n\n\n\n\n\n
\u30b5\u30f3\u30d7\u30eb<\/td>\n\u30d2\u30e9\u30e1<\/td>\n\u30d2\u30e9\u30e1<\/td>\n\u30de\u30c0\u30a4<\/td>\n<\/tr>\n
\u30b7\u30fc\u30b1\u30f3\u30b5\u30fc<\/td>\nMGI<\/td>\nMGI+Nanopore<\/td>\nMGI+Nanopore<\/td>\n<\/tr>\n
scafolds\u6570<\/td>\n62,791<\/td>\n1,839<\/td>\n1,938<\/td>\n<\/tr>\n
\u6700\u9577scaffolds(bp)<\/td>\n553,059<\/td>\n22,224,473<\/td>\n14,884,731<\/td>\n<\/tr>\n
N50(bp)<\/td>\n28,809<\/td>\n3,706,346<\/td>\n2,386,415<\/td>\n<\/tr>\n
\u7dcf\u5869\u57fa\u6570(bp)<\/td>\n565,690,090<\/td>\n590,887,377<\/td>\n789,901,210<\/td>\n<\/tr>\n<\/tbody>\n<\/table><\/div>\n

\u5f0a\u793e\u3067\u306f\u30d0\u30af\u30c6\u30ea\u30a2\u306e\u30b2\u30ce\u30e0\u89e3\u6790\u306b\u3064\u3044\u3066\u306f\u30cf\u30a4\u30d6\u30ea\u30c3\u30c9\u30a2\u30bb\u30f3\u30d6\u30eb\u3067\u30b3\u30f3\u30d7\u30ea\u30fc\u30c8\u3059\u308b\u3068\u3044\u3046\u306e\u304c\u65e5\u5e38\u7684\u306b\u306a\u308a\u3064\u3064\u3042\u308b\u306e\u3067\u3059\u304c\u3001\u3042\u308b\u7a0b\u5ea6\u30b2\u30ce\u30e0\u30b5\u30a4\u30ba\u304c\u5927\u304d\u306a\u751f\u7269\u3067\u30cf\u30a4\u30d6\u30ea\u30c3\u30c9\u30a2\u30bb\u30f3\u30d6\u30eb\u3092\u884c\u3063\u305f\u306e\u306f\u521d\u3081\u3066\u3067\u3059\u3002\u30cf\u30a4\u30d6\u30ea\u30c3\u30c9\u30a2\u30bb\u30f3\u30d6\u30eb\u306e\u5a01\u529b\u3092\u4f53\u611f\u3067\u304d\u307e\u3057\u305f\u3002\u4eca\u56de\u306e\u30c7\u30fc\u30bf\u306f\u65e5\u672c\u5927\u5b66\u751f\u7269\u8cc7\u6e90\u5b66\u90e8\u306e\u6fa4\u5c71\u82f1\u592a\u90ce\u69d8\u306e\u65b9\u3067\u9023\u9396\u5730\u56f3\u60c5\u5831\u3082\u5408\u308f\u305b\u3066\u30b9\u30ad\u30e3\u30db\u30fc\u30eb\u30c9\u306e\u3064\u306a\u304e\u5408\u308f\u305b\u3092\u884c\u3044\u3001\u3055\u3089RNA-seq\u306e\u30c7\u30fc\u30bf\u3082\u4f7f\u3063\u3066\u30a2\u30ce\u30c6\u30fc\u30b7\u30e7\u30f3\u3092\u884c\u3063\u3066\u3044\u304f\u4e88\u5b9a\u3067\u3059\u3002<\/p>\n

\u30cf\u30a4\u30d6\u30ea\u30c3\u30c9\u30a2\u30bb\u30f3\u30d6\u30eb\u306e\u5834\u5408nanopore\u306e\u30c7\u30fc\u30bf\u306e\u5fc5\u8981\u91cf\u306f\u30b2\u30ce\u30e0\u30b5\u30a4\u30ba\u306e25\u500d\u7a0b\u5ea6\u3067\u3059\u306e\u3067\u3001\u30ed\u30f3\u30b0\u30ea\u30fc\u30c9\u306e\u307f\u306750\u500d\u7a0b\u5ea6\u306e\u30c7\u30fc\u30bf\u91cf\u3092\u53d6\u5f97\u3059\u308b\u3088\u308a\u30cf\u30a4\u30d6\u30ea\u30c3\u30c9\u30a2\u30bb\u30f3\u30d6\u30eb\u306e\u65b9\u304c\u30c7\u30fc\u30bf\u53d6\u5f97\u30b3\u30b9\u30c8\u304c\u5b89\u304f\u306a\u308a\u307e\u3059\u3002\u307e\u305f\u3001\u3053\u3053\u6570\u304b\u6708\u3067nanopore\u306f\u6027\u80fd\u304c\u5411\u4e0a\u3057\u3066\u3044\u307e\u3059\u30021\u30d5\u30ed\u30fc\u30bb\u30eb\u3042\u305f\u308a10Gb\u304f\u3089\u3044\u306e\u30c7\u30fc\u30bf\u91cf\u304c\u5f97\u3089\u308c\u3066\u304a\u308a\uff08\u5f0a\u793e\u30c1\u30e3\u30f3\u30d4\u30aa\u30f3\u30c7\u30fc\u30bf\u306f20Gb\u3067\u3059\uff09\u3001\u9ad8\u54c1\u8cea\u306a\u30b2\u30ce\u30e0DNA\u3092\u30b5\u30f3\u30d7\u30eb\u3068\u3057\u305f\u5834\u5408\u3001\u6700\u9577\u30ea\u30fc\u30c9\u306e\u30ea\u30fc\u30c9\u9577\u304c100-200kb\u3068\u306a\u3063\u3066\u3044\u307e\u3059\u3002<\/p>\n

MGISEQ2000\u3092\u4f7f\u3063\u305f\u89e3\u6790\u30b5\u30fc\u30d3\u30b9\u306f\u304a\u76c6\u660e\u3051\u306b\u30b9\u30bf\u30fc\u30c8\u3059\u308b\u4e88\u5b9a\u3067\u3059\u3002\u4eca\u6708\u4e2d\u306b\u30b5\u30fc\u30d3\u30b9\u5185\u5bb9\u3092\u6c7a\u5b9a\u3057\u30018\u67087\u65e5\u304b\u3089\u5317\u6d77\u9053\u5927\u5b66\u3067\u958b\u50ac\u3055\u308c\u308b\u9032\u5316\u5b66\u4f1a\u3067\u4e8b\u524d\u306ePR\u3092\u3055\u305b\u3066\u3044\u305f\u3060\u304f\u4e88\u5b9a\u3067\u3059\u3002\u9032\u5316\u5b66\u4f1a\u306b\u53c2\u52a0\u3055\u308c\u308b\u65b9\u306f\u662f\u975e\u3068\u3082\u5f0a\u793e\u306e\u30d6\u30fc\u30b9\u306b\u304a\u7acb\u3061\u5bc4\u308a\u304f\u3060\u3055\u3044\u3002<\/p>\n

\u793e\u5c4b\u79fb\u8ee2\u3068MGISEQ2000\u306e\u5c0e\u5165\u3067\u304a\u91d1\u3092\u4f7f\u3044\u679c\u305f\u3057\u3066\u3057\u307e\u3063\u305f\u306e\u3067\u3001\u3053\u308c\u304b\u3089\u306f\u81ea\u8ee2\u8eca\u64cd\u696d\u306e\u65e5\u3005\u3067\u3059\u2192\u8da3\u5473\u306e\u81ea\u8ee2\u8eca\u3082\u5fa9\u6d3b\u3057\u307e\u3057\u305f\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"\u6765\u9031\u65b0\u3057\u3044\u30b7\u30fc\u30b1\u30f3\u30b5\u30fc\u3092\u5c0e\u5165\u3057\u307e\u3059\u3002\u30a4\u30eb\u30df\u30ca\u793e\u306e\u30b7\u30fc\u30b1\u30f3\u30b5\u30fc\u3067\u306f\u306a\u304f\u4e2d\u56fd\u88fd\u306eMGISEQ2000\u3068\u3044\u3046\u6a5f\u7a2e\u3067\u3059\u3002 \u306a\u3093\u306e\u3053\u3063\u3061\u3083\uff1f\u3063\u3066\u3044\u3046\u4eba\u304c\u307b\u3068\u3093\u3069\u3060\u3068\u601d\u3044\u307e\u3059\u304c\u3001\u5b9f\u306f\u6a2a\u6d5c\u306e\u7406\u5316\u5b66\u7814\u7a76\u6240\u306b\u306f3\u53f0\u5c0e\u5165\u3055\u308c\u3066\u304a\u308a\u3001\u4e00\u5e74\u4ee5\u4e0a […]","protected":false},"author":2,"featured_media":4203,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[10],"tags":[],"class_list":["post-3200","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-10"],"_links":{"self":[{"href":"https:\/\/gikenbio.com\/2025test\/wp-json\/wp\/v2\/posts\/3200","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/gikenbio.com\/2025test\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/gikenbio.com\/2025test\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/gikenbio.com\/2025test\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/gikenbio.com\/2025test\/wp-json\/wp\/v2\/comments?post=3200"}],"version-history":[{"count":19,"href":"https:\/\/gikenbio.com\/2025test\/wp-json\/wp\/v2\/posts\/3200\/revisions"}],"predecessor-version":[{"id":3220,"href":"https:\/\/gikenbio.com\/2025test\/wp-json\/wp\/v2\/posts\/3200\/revisions\/3220"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/gikenbio.com\/2025test\/wp-json\/wp\/v2\/media\/4203"}],"wp:attachment":[{"href":"https:\/\/gikenbio.com\/2025test\/wp-json\/wp\/v2\/media?parent=3200"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/gikenbio.com\/2025test\/wp-json\/wp\/v2\/categories?post=3200"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/gikenbio.com\/2025test\/wp-json\/wp\/v2\/tags?post=3200"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}